Atmospheric-pressure ionization and fragmentation of peptides by solution-cathode glow discharge

Schwartz, Andrew J.; Shelley, Jacob T.; Walton, Courtney L.; Williams, Kelsey L.; Hieftje, Gary M.

doi:10.1039/c6sc02032a

Cited by 26 publications

(25 citation statements)

References 47 publications

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“…FAPA, or SCGD) can be tuned in 'hardness', enabling them to produce mainly molecular ions, fragment ions or atomic mass spectra, alternatively [150]. They are being evaluated at present to provide information about the amino-acid sequence of proteins and about the post-translational modification of proteins [151].…”

Section: Advances In Science and Technology To Meet Challengesmentioning

confidence: 99%

The 2017 Plasma Roadmap: Low temperature plasma science and technology

Adamovich

Baalrud

Bogaerts

et al. 2017

J. Phys. D: Appl. Phys.

815

549

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Section: Advances In Science and Technology To Meet Challengesmentioning

confidence: 99%

The 2017 Plasma Roadmap: Low temperature plasma science and technology

Adamovich

Baalrud

Bogaerts

et al. 2017

J. Phys. D: Appl. Phys.

815

549

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“…Unlike the LS-APGD, no changes to the support solution composition were required to generate the biomolecular ions. Furthermore, the SCGD-MS results were significantly different from those obtained for biomolecular analysis with the LS-APGD [18,20]. Analysis of myoglobin by LS-APGD yielded an ESI-like charge-state distribution and no fragment ions, apart from loss of the heme group.…”

Section: Introductionmentioning

confidence: 58%

“…Earlier work with the SCGD as an ionization source for biomolecular mass spectrometry [20] revealed that fragmentation is tunable by adjustment of the discharge current. Similar results were observed here for small molecular species introduced within the liquid stream.…”

Section: Molecular Mass Spectra From Solutionsmentioning

confidence: 99%

“…Though extensive work has been performed to characterize the LS-APGD as an ion source, MS applications of the SCGD are only now being pursued. A recent study by Schwartz et al [20] revealed that the SCGD efficiently produces gas-phase ions of labile biomolecules (peptides) directly from aqueous solution. Ionization and fragmentation of the peptides was controllably tunable through changes in the discharge current.…”

Section: Introductionmentioning

confidence: 99%

“…Shown in Figure 1 is a photograph of the SCGD cell and capillary mass-spectrometer interface used for ion sampling. The design of the SCGD cell and interface is identical to that described earlier [20], so only a brief description to indicate differences in parameters will be provided here. In the SCGD of Figure 1, electrolyte solution (either 0.1 M HNO 3 or HCl) is pumped into the cell through a vertically oriented glass capillary at a rate of 1.7 mL min -1 .…”

Section: Scgd Cell Ion Sampling and Sample Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Atmospheric-pressure solution-cathode glow discharge: A versatile ion source for atomic and molecular mass spectrometry

Schwartz

Williams

Hieftje

et al. 2017

Analytica Chimica Acta

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An atmospheric-pressure solution-cathode glow discharge (SCGD) has been evaluated as an ion source for atomic, molecular, and ambient desorption/ionization mass spectrometry. The SCGD consists of a direct-current plasma, supported in the ambient air in the absence of gas flows, and sustained upon the surface of a flowing liquid cathode. Analytes introduced in the flowing liquid, as an ambient gas, or as a solid held near the plasma are vaporized and ionized by interactions within or near the discharge. Introduction of acidic solutions containing metal salts produced bare elemental ions as well as HO, OH and NO adducts. Detection limits for these elemental species ranged from 0.1 to 4 ppb, working curves spanned more than 4 orders of linear dynamic range, and precision varied between 5 and 16% relative standard deviation. Small organic molecules were also efficiently ionized from solution, and both the intact molecular ion and fragments were observed in the resulting SCGD mass spectra. Fragmentation of molecular species was found to be tunable; high discharge currents led to harder ionization, while low discharge currents produced stronger molecular-ion signals. Ambient gases and solids, desorbed by the plasma from a glass probe, were also readily ionized by the SCGD. Indeed, strong analyte signals were obtained from solid samples placed at least 2 cm from the plasma. These findings indicate that the SCGD might be useful also for ambient desorption/ionization mass spectrometry. Combined with earlier results that showed the SCGD is useful for ionization of labile biomolecules, the results here indicate that the SCGD is a highly versatile ion source capable of providing both elemental and molecular mass-spectral information.

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Modern Plasma‐based Desorption/Ionization: From Atoms and Molecules to Chemical Synthesis

Molnar

Shelley

2020

Mass Spectrometry Reviews

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Since the first mass spectrometry (MS) experiments were conducted by Thomson and Aston, plasmas have been used as ionization sources. Historically, plasma ion sources were used for these experiments because they were one of the few known sources of gasphase ions at the time and they were relatively simple to setup and operate. Since then, developments in plasma ionization have continued to inform and motivate advances in other areas of MS. For example, plasma-desorption MS demonstrated ionization of large peptides and polymers more than 10 years before the first descriptions of electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI). As a result, significant effort was placed on development of ionization approaches, mass analysis, and detection approaches for very large molecules: even before the advent of ESI and MALDI. Since then, new analytical challenges and opportunities in plasma ionization have arisen. In this review, the emerging trends in plasma-based ionization for several areas of MS will be discussed, including molecular ionization, elemental ionization, hybrid elemental and molecular ion sources, and unique chemical transformations.

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Atmospheric-pressure ionization and fragmentation of peptides by solution-cathode glow discharge

Abstract: Ionization and tunable fragmentation of peptides at atmospheric pressure was achieved by means of a solution-cathode glow discharge ionization source.

Cited by 26 publications

References 47 publications

The 2017 Plasma Roadmap: Low temperature plasma science and technology

The 2017 Plasma Roadmap: Low temperature plasma science and technology

Atmospheric-pressure solution-cathode glow discharge: A versatile ion source for atomic and molecular mass spectrometry

Modern Plasma‐based Desorption/Ionization: From Atoms and Molecules to Chemical Synthesis

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