Atmospheric pressure plasma in dermatology: Ulcus treatment and much more

Emmert, Steffen; Brehmer, Franziska; Hänßle, Holger; Helmke, Andreas; Mertens, Nina; Ahmed, Raees; Simon, Dirk; Wandke, Dirk; Maus‐Friedrichs, W.; Daschlein, Georg; Schön, Michael P.; Viöl, Wolfgang

doi:10.1016/j.cpme.2012.11.002

Cited by 174 publications

(135 citation statements)

References 43 publications

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“…Sterilization of medical materials or devices (e.g., medical implants, catheters, or materials in blood purification systems) is the main use of indirect plasmas for medical purposes; 20 whereas physical plasmas are under intense study for applications in wound healing, 19,20 blood coagulation and skin regeneration, 21 dentistry, 22 and apoptosis of cancer cells. 23 To date, research on ACP sterilization has demonstrated effective reduction of clinically important microorganisms in their planktonic state or in biofilms. 24,25 However, the antimicrobial effects of plasma were reduced against biofilms by comparison with their planktonic counterparts.…”

Section: Introductionmentioning

confidence: 99%

Dielectric Barrier Discharge Atmospheric Cold Plasma for Inactivation of Pseudomonas aeruginosa Biofilms

Ziuzina¹,

Patil²,

Cullen

et al. 2014

Plasma Med

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ABSTRACT:In recent years, atmospheric cold plasma (ACP) has been widely investigated for potential application as an alternative decontamination technology in biomedical and healthcare sectors. In this study, the antimicrobial efficacy of ACP against Pseudomonas aeruginosa biofilms was investigated. The 48-h biofilms were treated inside sealed polypropylene containers with a high-voltage dielectric barrier discharge (DBD) ACP (80 kV RMS ) and subsequently stored for 24 h at room temperature. Treatment for 60 s by either the direct or indirect mode of ACP exposure (inside or outside plasma discharge, respectively) reduced bacterial populations by an average of 5.4 log cycles from an initial 6.6 log 10 CFU/mL. Increasing the treatment time from 60 s to 120 s and 300 s reduced biofilms to undetectable levels. According to XTT assay (a metabolic activity assay), an extended treatment time of 300 s was necessary to reduce metabolic activity of cells in biofilms by an average of 70%. Further investigation of biofilm viability by confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM) demonstrated that extended ACP treatment had a detrimental effect on the viability of P. aeruginosa through disintegration of both bacterial cells and the biofilm matrix. The results of this study demonstrate the potential of a novel, in-package, high-voltage ACP decontamination approach for the inactivation of bacterial biofilms.

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Section: Introductionmentioning

confidence: 99%

Dielectric Barrier Discharge Atmospheric Cold Plasma for Inactivation of Pseudomonas aeruginosa Biofilms

Ziuzina¹,

Patil²,

Cullen

et al. 2014

Plasma Med

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“…Nabrojeni učinci su posljedica djelovanja ROS-a i RNS/RONS-a, kao i plazmom induciranih promjena u vlažnom okolišu stanica i tkiva 5 . Uz tretiranje kroničnih rana, u novije vrijeme objavljuju se rezultati primjene CAP-a i u drugim područjima dermatologije, kao što su ublažava-nje bolova i ubrzavanje liječenja herpes zostera te tretiranje ekcema i psorijaze [38][39][40] .…”

Section: Dermatologijaunclassified

Plasma applications in medicine and dentistry

Mance

2017

Med. flum.

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Sažetak. U fizici, plazma je ioniziran, kvazineutralan plin za koji se često kaže da je četvrto stanje materije. Dugo vremena se plazma u medicini koristila zbog svojih termalnih svojstava, primjerice za sterilizaciju kirurškog i medicinskog pribora te za rezanje i kauterizaciju tkiva. Generiranjem hladne plazme u atmosferskim uvjetima započelo je novo razdoblje u primjeni plazme u biomedicini. Plazma medicina novo je interdisciplinarno znanstveno područje koje kombinira spoznaje fizike plazme s biomedicinskim i kliničkim znanostima. Hladna atmosferska plazma emitira elektromagnetno zračenje te se sastoji od mnoštva nabijenih i nenabijenih čestica, među kojima su najznačajnije reaktivne kisikove i dušikove vrste. Smatra se da su upravo reaktivne kisikove vrste najvažnije za biološke učinke plazme kao što su inaktivacija mikroorganizama i biofilmova. U dermatologiji se pokazalo da hladna atmosferska plazma pozitivno utječe na zacjeljivanje kroničnih rana. Prvi instrumenti za primjenu hladne plazme na ljudima već su i komercijalno dostupni. Primjena hladne plazme ima velik potencijal za primjenu u onkologiji, ali i u stomatologiji. Mnogobrojne studije dale su vrlo obećavajuće rezultate za primjenu hladne plazme u medicini, no treba imati na umu da mehanizmi međudjelovanja plazme s biološkim tkivom još uvijek nisu u potpunosti razjašnjeni, da nema dogovorom prihvaćene definicije doze koju plazma predaje materiji, da još uvijek nema standardiziranih instrumenata za generiranje i karakterizaciju plazme te da za pravu procjenu potencijala primjene atmosferske plazme u medicini i stomatologiji nedostaje više detaljnih in vivo studija.Ključne riječi: dermatologija; onkologija; plazma; stomatologija Abstract. Physical plasma is an ionized, quasi-neutral gas. It is often said to be the fourth state of matter. For a long time, plasma was used in medicine exclusively because of its thermal properties for sterilization purposes of surgical equipment and medical supplies, and also to cut and cauterize tissue. The possibility to generate cold plasma in atmospheric conditions marked the beginning of a completely new era in the application of plasma in biomedicine. Plasma medicine is a new interdisciplinary field of science that combines the knowledge of plasma physics to biomedical and clinical sciences. Cold atmospheric plasma emits electromagnetic radiation and it consists out of charged and uncharged particles, among those reactive oxygen and nitrogen species are the most important ones. It is considered that reactive oxygen species cause the most important biological plasma effects, such as inactivation of microorganisms and biofilms. It has been shown in dermatology that cold atmospheric plasma has a healing effect on chronic wounds. First instruments for the application of cold plasma in humans are already commercially available. Cold plasma has a great potential for application in oncology and in dentistry. Numerous studies have yielded very promising results for the application of cold plasma in medicine, but it should ...

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“…A principal diferença entre um gás e o plasma é o fato do plasma ser condutor de eletricidade devido à presença de cargas elétricas livres (elétrons e íons) entre seus constituintes, que fazem o plasma ser eletricamente condutor e responder fortemente a campos eletromagnéticos (FRIDMAN, 2008). No plasma térmico, as espécies quimicamente ativas e altamente energéticas estão em equilíbrio termodinâmico com o gás circundante (STAACK, 2008), (EMMERT et al, 2013). Existem inúmeros exemplos de aplicações da tecnologia de plasma, entre eles: a degradação de gases, reações de síntese, modificações e tratamento de superfícies, produção de nanoestruturas, entre outros (CUBAS et al, 2005) (SAMAL; PARK, 2012), (BENETOLI et al, 2012), (XU et al, 2013).…”

Section: Introductionunclassified

Produção de Hidrogênio e Negro de Carbono a partir da Degradação de Metano por Plasma Térmico

et al. 2014

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ResumoO gás metano (CH 4 ) é um dos principais gases indutores do efeito estufa. Pesquisas científicas recentes visam minimizar o acúmulo deste gás na atmosfera e desenvolver processos capazes de produzir materiais estáveis com valor agregado. O plasma térmico é uma alternativa promissora para alcançar tais objetivos, pois possibilita a obtenção de H 2 e carbono sólido a partir do CH 4 , sem a formação paralela de subprodutos como CO 2 e NO x . Neste trabalho, o CH 4 foi degradado por plasma térmico visando a produção de hidrogênio (H 2 ) e negro de carbono. A eficiência de degradação do CH 4 , o rendimento do processo e a caracterização do negro de carbono produzido foram estudados. Os melhores resultados obtidos foram no fluxo de 5 L min -1 de CH 4 a porcentagem de degradação e a seletividade para a produção de H 2 chegam a 98,8 % e 48,4 %, respectivamente. Em fluxos menores que 5 L min -1 , o rendimento na produção de H 2 aumenta e chega a 91,9 %. O negro de carbono obtido apresenta estrutura amorfa, características hidrofóbicas e pode ser comercializado como material para compósitos, podendo ainda ser ativado química e/ou fisicamente e utilizado como material adsorvente. Palavras-chave: Plasma térmico. Metano. Hidrogênio. Negro de carbono. AbstractMethane gas (CH 4 ) is the main inducer of the so called greenhouse gases effect. Recent scientific research aims to minimize the accumulation of this gas in the atmosphere and to develop processes capable of producing stable materials with added value. Thermal plasma technology is a promising alternative to these applications, since it allows obtaining H 2 and solid carbon from CH 4 , without the parallel formation of byproducts such as CO 2 and NO x . In this work, CH 4 was degraded by thermal plasma in order to produce hydrogen (H 2 ) and carbon black. The degradation efficiency of CH 4 , selectivity for H 2 production as well as the characterization of carbon black were studied. The best results were obtained in the CH 4 flow rate of 5 L min -1 the degradation percentage and the selectivity for H 2 production reached 98.8 % and 48.4 %, respectively. At flow rates of less than 5 L min -1 the selectivity for H 2 production increases and reaches 91.9 %. The carbon black has obtained amorphous with hydrophobic characteristics and can be marketed to be used in composite material, and can also be activated chemically and/or physically and used as adsorbent material.

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Atmospheric pressure plasma in dermatology: Ulcus treatment and much more

Cited by 174 publications

References 43 publications

Dielectric Barrier Discharge Atmospheric Cold Plasma for Inactivation of Pseudomonas aeruginosa Biofilms

Dielectric Barrier Discharge Atmospheric Cold Plasma for Inactivation of Pseudomonas aeruginosa Biofilms

Plasma applications in medicine and dentistry

Produção de Hidrogênio e Negro de Carbono a partir da Degradação de Metano por Plasma Térmico

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