Atmospheric pressure plasma jet—Living tissue interface: Electrical, optical, and spectral characterization

Nastuta, Andrei Vasile; Pohoaţǎ, Valentin; Topală, Ionuț

doi:10.1063/1.4804319

Cited by 35 publications

(23 citation statements)

References 46 publications

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“…More specifically, the produced GIWs reached maximum lengths of up to 4 cm for low duty cycle values (1-2%) and high amplitudes of the applied voltage (8 kV) and frequency (15)(16)(17)(18)(19)(20). Besides, T sat varied between 25 and 80 • C, while it increased linearly with the voltage amplitude.…”

Section: Discussionmentioning

confidence: 94%

Electrical, Thermal and Optical Parametric Study of Guided Ionization Waves Produced with a Compact μs-Pulsed DBD-Based Reactor

Doanh

Held

Clément

2017

Plasma

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Atmospheric pressure guided ionization waves (GIWs) that are driven by ns/µs-pulsed high voltages, are promising tools in the biomedical field allowing for the effective production of reactive species and metastables without thermal damages of the specimens that are exposed. In most cases, plasma is produced in noble gases using dielectric barrier discharge (DBD) devices of more-or-less sophisticated geometries. In this study, a compact low-cost DBD reactor of very simple geometry is presented. It is fed with pure helium and driven by positive µs-pulsed high voltage (amplitude: 4.5-8 kV, pulse width: 1-10 µs) of audio frequencies (5-20 kHz), while it operates consistently for long time periods in a wide range of conditions. The produced plasma exhibits propagation lengths up to 4 cm and rich chemical reactivity is established outside the reactor, depending on the device's experimental parameters. Besides, the dielectric tube's temperature during plasma operation is an important factor, which is linked to the plasma characteristics. This temperature and its variations are thoroughly investigated herein, along with GIWs electrical features versus the electrical parameters of the pulsed power supply. Accordingly, it is demonstrated that not all of the operational windows are adequate for thermal-free operation and suitable operating conditions of this system are proposed for diverse applications, such as biomedical (low gas temperature is a prerequisite) and surface treatments of solid materials (low temperatures are not required).

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Section: Discussionmentioning

confidence: 94%

Electrical, Thermal and Optical Parametric Study of Guided Ionization Waves Produced with a Compact μs-Pulsed DBD-Based Reactor

Doanh

Held

Clément

2017

Plasma

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“…One possible way to generate plasma without direct contact from the HV electrode to the target may be achieved by combination of perpendicularly set capillary tubes, where the plasma generated in one tube is capable to generate secondary ionization wave in another spatially separated and electrodeless tube by so called plasma transfer phenomenon. 58,59 For these advantages, plasma jets are being extensively investigated and tested for many biomedical applications in direct contact with living cells 9,60,61 and tissues, e.g., for skin and wound sterilization, [62][63][64][65] or cancer treatment. 33,[66][67][68] The objective of this paper is to demonstrate some effects of cold plasmas on bacteria, eukaryotic cells, and selected biomolecules that are related to bio-decontamination and potential cancer therapies.…”

Section: Introductionmentioning

confidence: 99%

Effects of air transient spark discharge and helium plasma jet on water, bacteria, cells, and biomolecules

et al. 2015

Self Cite

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Atmospheric pressure DC-driven self-pulsing transient spark (TS) discharge operated in air and pulse-driven dielectric barrier discharge plasma jet (PJ) operated in helium in contact with water solutions were used for inducing chemical effects in water solutions, and the treatment of bacteria (Escherichia coli), mammalian cells (Vero line normal cells, HeLa line cancerous cells), deoxyribonucleic acid (dsDNA), and protein (bovine serum albumin). Two different methods of water solution supply were used in the TS: water electrode system and water spray system. The effects of both TS systems and the PJ were compared, as well as a direct exposure of the solution to the discharge with an indirect exposure to the discharge activated gas flow. The chemical analysis of water solutions was performed by using colorimetric methods of UV-VIS absorption spectrophotometry. The bactericidal effects of the discharges on bacteria were evaluated by standard microbiological plate count method. Viability, apoptosis and cell cycle were assessed in normal and cancerous cells. Viability of cells was evaluated by trypan blue exclusion test, apoptosis by Annexin V-FITC/propidium iodide assay, and cell cycle progression by propidium iodide/RNase test. The effect of the discharges on deoxyribonucleic acid and protein were evaluated by fluorescence and UV absorption spectroscopy. The results of bacterial and mammalian cell viability, apoptosis, and cell cycle clearly show that cold plasma can inactivate bacteria and selectively target cancerous cells, which is very important for possible future development of new plasma therapeutic strategies in biomedicine. The authors found that all investigated bio-effects were stronger with the air TS discharge than with the He PJ, even in indirect exposure.

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“…Since we're using a single electrode in this situation, the subject might be considered a floating electrode and, for the 15 mm gap, what we might be seeing is the incomplete discharge for the pre-bullet, resulting in the decreased OES intensities. Nastuta et al tested atmospheric pressure at the plasma jet-living tissue interface at 5 mm and 15 mm and found similar drops in intensity for subjects [31].…”

Section: Resultsmentioning

confidence: 86%

Tiny Cold Atmospheric Plasma Jet for Biomedical Applications

2021

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Conventional plasma jets for biomedical applications tend to have several drawbacks, such as high voltages, high gas delivery, large plasma probe volume, and the formation of discharge within the organ. Therefore, it is challenging to employ these jets inside a living organism’s body. Thus, we developed a single-electrode tiny plasma jet and evaluated its use for clinical biomedical applications. We investigated the effect of voltage input and flow rate on the jet length and studied the physical parameters of the plasma jet, including discharge voltage, average gas and subject temperature, and optical emissions via spectroscopy (OES). The interactions between the tiny plasma jet and five subjects (de-ionized (DI) water, metal, cardboard, pork belly, and pork muscle) were studied at distances of 10 mm and 15 mm from the jet nozzle. The results showed that the tiny plasma jet caused no damage or burning of tissues, and the ROS/RNS (reactive oxygen/nitrogen species) intensity increased when the distance was lowered from 15 mm to 10 mm. These initial observations establish the tiny plasma jet device as a potentially useful tool in clinical biomedical applications.

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Atmospheric pressure plasma jet—Living tissue interface: Electrical, optical, and spectral characterization

Cited by 35 publications

References 46 publications

Electrical, Thermal and Optical Parametric Study of Guided Ionization Waves Produced with a Compact μs-Pulsed DBD-Based Reactor

Electrical, Thermal and Optical Parametric Study of Guided Ionization Waves Produced with a Compact μs-Pulsed DBD-Based Reactor

Effects of air transient spark discharge and helium plasma jet on water, bacteria, cells, and biomolecules

Tiny Cold Atmospheric Plasma Jet for Biomedical Applications

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