Atmospheric Pressure Pulsed Plasma Induces Cell Death in Photosynthetic Organs via Intracellularly Generated ROS

Seol, You Bin; Kim, Jaewook; Park, Se-Hong; Chang, Hong Young

doi:10.1038/s41598-017-00480-6

Cited by 28 publications

(11 citation statements)

References 41 publications

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“…Transport also depends on the life stage of the plant since ozone can be taken up through the stomata in the leaves. Seol et al [149] observed an accumulation of ROS in chloroplasts, and suggested that through the micropores, ROS can travel down further into the plant tissue, from the epidermis into the mesophyll but if too potent, chloroplast degradation occurs.…”

Section: Ros Entry and Involvement In Seed Developmentmentioning

confidence: 99%

Mechanisms of Plasma-Seed Treatments as a Potential Seed Processing Technology

2021

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Plasma treatments are currently being assessed as a seed processing technology for agricultural purposes where seeds are typically subjected to pre-sowing treatments to improve the likelihood of timely and uniform germination. The aim of this review is to summarize the hypotheses and present the evidence to date of how plasma treatments affect seeds, considering that there is difficulty in standardizing the methodology in this interdisciplinary field given the plethora of variables in the experimental setup of the plasma device and handling of biological samples. The ever increasing interest for plasma agriculture drives the need for a review dedicated to seeds, which is understandable to an interdisciplinary audience of biologists and plasma physicists. Seeds are the first step of the agricultural cycle and at this stage, the plant can be given the highest probability of establishment, despite environmental conditions, to exploit the genetic potential of the seed. Furthermore, seedlings seem to be too sensitive to the oxidation of plasma and therefore, seeds seem to be the ideal target. This review intentionally does not include seed disinfection and sterilization due to already existing reviews. Instead, a summary of the mechanisms of how plasma may be affecting the seed and its germination and developmental properties will be provided and discussed.

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Section: Ros Entry and Involvement In Seed Developmentmentioning

confidence: 99%

Mechanisms of Plasma-Seed Treatments as a Potential Seed Processing Technology

2021

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“…This suggests that high-intensity plasma treatments may cause the inhibitory effects on fungal cellular processes, which may be related to the concentration of ROS and NOx produced by plasma. With the increase in the number and intensity of the plasma treatments, the ROS and NOx concentrations in the media increase continuously, which will induce cell death [ 44 ]. Possibly, there is a plasma dose threshold for the activation of the enzyme production.…”

Section: Discussionmentioning

confidence: 99%

Plasma Promotes Fungal Cellulase Production by Regulating the Levels of Intracellular NO and Ca2+

Ketya

Choi

et al. 2022

IJMS

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For the industrial-scale production of useful enzymes by microorganisms, technological development is required for overcoming a technical bottleneck represented by poor efficiency in the induction of enzyme gene expression and secretion. In this study, we evaluated the potential of a non-thermal atmospheric pressure plasma jet to improve the production efficiency of cellulolytic enzymes in Neurospora crassa, a filamentous fungus. The total activity of cellulolytic enzymes and protein concentration were significantly increased (1.1~1.2 times) in media containing Avicel 24–72 h after 2 and 5 min of plasma treatment. The mRNA levels of four cellulolytic enzymes in fungal hyphae grown in media with Avicel were significantly increased (1.3~17 times) 2–4 h after a 5 min of plasma treatment. The levels of intracellular NO and Ca2+ were increased in plasma-treated fungal hyphae grown in Avicel media after 48 h, and the removal of intracellular NO decreased the activity of cellulolytic enzymes in media and the level of vesicles in fungal hyphae. Our data suggest that plasma treatment can promote the transcription and secretion of cellulolytic enzymes into the culture media in the presence of Avicel (induction condition) by enhancing the intracellular level of NO and Ca2+.

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“…In a previous study, germination was inhibited significantly by a treatment of 50 V input for 9 s [ 6 ]. The reason may be that this treatment exceeded the high dose for living tissue [ 8 ], and excessive ROS produced by plasma may cause damage to seeds [ 49 ].…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic analysis of seed germination improvement of Andrographis paniculata responding to air plasma treatment

Tong

Tang

et al. 2020

PLoS ONE

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The plasma seed treatment is effective for promoting seed germination in many crops. However, the biological mechanism remains unclear. Therefore, mRNA sequencing was used to screen differentially expressed genes in the germination process of Andrographis paniculata seeds treated with air plasma (power density = 8.99 J/cm 3 ). Following plasma treatment, the germination percentages were significantly higher than those of the control, they were 3.5±0.6% vs . 0 at 28 hours after sowing (HAS) and 50.3±2.6% vs . 37.3±1.7% at 48 HAS. After unigenes were assembled and annotated, 125 differentially expressed genes (DEGs) were detected at 28 HAS, compared with nine DEGs at 48 HAS, but no DEGs were detected at 0 HAS, indicating that air plasma treatment mainly changed the gene expression of A . paniculata seeds at 28 HAS. The NCED5 expression level of the treated group was less than one-fifth of the control, and the expressions of three ethylene response factors were significantly higher than the control at 28 HAS, indicating that lower abscisic acid levels play an important role and ethylene signal transduction also participates in radicle protrusion. ACO , NRT1 and PRP3 expressions were significantly higher than in the control at 48 HAS, suggesting that higher ethylene levels cause the endosperm cap to weaken and start to grow root hairs and lateral roots earlier. These findings reveal that plasma promotes seed germination mainly by regulating the expression of hormone-related genes. And the possible signal transduction of related hormones was discussed.

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Atmospheric Pressure Pulsed Plasma Induces Cell Death in Photosynthetic Organs via Intracellularly Generated ROS

Cited by 28 publications

References 41 publications

Mechanisms of Plasma-Seed Treatments as a Potential Seed Processing Technology

Mechanisms of Plasma-Seed Treatments as a Potential Seed Processing Technology

Plasma Promotes Fungal Cellulase Production by Regulating the Levels of Intracellular NO and Ca2+

Transcriptomic analysis of seed germination improvement of Andrographis paniculata responding to air plasma treatment

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