AtNOT1 Is a Novel Regulator of Gene Expression during Pollen Development

Motomura, Kazuki; Arae, Toshihiro; Araki-Uramoto, Haruka; Suzuki, Yoshishige; Takeuchi, Hidenori; Suzuki, Takamasa; Ichihashi, Yasunori; Shibata, Arisa; Shirasu, Ken; Takeda, Atsushi; Higashiyama, Tetsuya; Chiba, Yasuhiro

doi:10.1093/pcp/pcz235

Cited by 13 publications

(10 citation statements)

References 48 publications

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“…Interestingly NOT1, the scaffold protein of the CCR4-NOT complex, has been recently established as an important player during male and female gametophyte development in Arabidopsis, with its disruption showing abnormal seed set [170,171]. Notably, the variant 949 G>A was also found in a homozygous state in Chasselas apyrène (Additional file 1: Table S13), which may further support its role in the seedless phenotype.…”

Section: Genes With Validated Snps Between Sangiovese and Corinto Neromentioning

confidence: 85%

Mechanisms and Candidate Genes for Seed and Fruit Set in Grapevine

Costantini¹,

Nwafor²,

Lorenzi³

et al. 2020

Preprint

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Background: Grapevine reproductive development has direct implications on yield. It also impacts on berry and wine quality by affecting traits like cluster compactness, bunch and berry size, berry skin to pulp ratio or seedlessness. Seasonal fluctuations in yield, fruit composition and wine attributes, which are largely driven by climatic factors, are major challenges for worldwide table grape and wine industry. Accordingly, a better understanding of reproductive processes such as gamete development, fertilization, seed and fruit set is of paramount relevance for managing yield and quality. With the aim of providing new insights into this field, we searched for clones with contrasting seed content in two germplasm collections. Results: We identified eight variant pairs that seemingly differ only in seed-related characteristics while showing identical genotype when tested with the GrapeReSeq_Illumina_20K_SNP_chip and several microsatellites. We performed multi-year observations on fruit and seed set deriving from different pollination treatments, with special emphasis on the pair composed by Sangiovese and its seedless variant locally named Corinto Nero. The pollen of Corinto Nero failed to germinate in vitro and gave poor berry set when used to pollinate other varieties. Most berries from both open- and cross-pollinated Corinto Nero inflorescences did not contain seeds. The genetic analysis of seedlings derived from occasional Corinto Nero normal seeds revealed that the few Corinto Nero functional gametes are mostly unreduced. A number of genes potentially involved in sporogenesis and gametogenesis showed contrasting expression between Corinto Nero and Sangiovese and five missense single nucleotide polymorphisms were identified from transcriptomic data. The above findings suggest that the seedless phenotype of Corinto Nero is driven by pollen and/or embryo sac defects, and both events likely arise from meiotic anomalies. Finally, three genotypes, including Sangiovese and Corinto Nero, were unexpectedly found to develop fruits without pollen contribution and occasionally showed normal-like seeds. Conclusions: Our collective results suggest that parthenocarpy and stenospermocarpy are not restricted to Black Corinth (alias Korinthiaki) and Sultanina-derived cultivars. The single nucleotide polymorphisms identified between Sangiovese and its parthenocarpic variant Corinto Nero are suitable for testing as traceability markers for propagated material and as functional candidates for the seedless phenotype.

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Section: Genes With Validated Snps Between Sangiovese and Corinto Neromentioning

confidence: 85%

Mechanisms and Candidate Genes for Seed and Fruit Set in Grapevine

Costantini¹,

Nwafor²,

Lorenzi³

et al. 2020

Preprint

View full text Add to dashboard Cite

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“…RNA exosomes or decapping proteins are key enzymes of RNA metabolism that are ubiquitously expressed during the transition of cell identity and function through RNA degradation. Mutants of these factors show severe developmental defects during gametogenesis and embryogenesis ( Motomura et al., 2012 ; Kumakura et al., 2013 ; Motomura et al., 2020 ). Pollen tube growth also accompanies drastic changes in the gene expression profile and morphology; however, no phenotypes have been reported in these mutant pollen tubes.…”

Section: Discussionmentioning

confidence: 99%

Possible molecular mechanisms of persistent pollen tube growth without de novo transcription

et al. 2022

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The vegetative cell nucleus proceeds ahead of a pair of sperm cells located beneath the pollen tube tip during germination. The tip-localized vegetative nucleus had been considered to play a pivotal role in the control of directional pollen tube growth and double fertilization. However, we recently reported the female-targeting behavior of pollen tubes from mutant plants, of which the vegetative nucleus and sperm nuclei were artificially immotile. We showed that the apical region of the mutant pollen tubes became physiologically enucleated after the first callose plug formation, indicating the autonomously growing nature of pollen tubes without the vegetative nucleus and sperm cells. Thus, in this study, we further analyzed another Arabidopsis thaliana mutant producing physiologically enucleated pollen tubes and discussed the mechanism by which a pollen tube can grow without de novo transcription from the vegetative nucleus. We propose several possible molecular mechanisms for persistent pollen tube growth, such as the contribution of transcripts before and immediately after germination and the use of persistent transcripts, which may be important for a competitive race among pollen tubes.

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“…AtNOT1 is necessary for e.g. proper male germ cell development, pollen germination and embryogenesis (Motomura et al ., 2020; Pereira et al ., 2020). A moss homolog of MBD9, a SWR1-C interacting protein required for H2A.Z deposition at a subset of actively transcribing genes in Arabidopsis (Potok et al ., 2019; Luo et al ., 2020), also show elevated expression in the three selected tissue types.…”

Section: Discussionmentioning

confidence: 99%

MS1/MMD1homologs in the mossP. patensare required for male and female gametogenesis and likely for sporogenesis

Landberg

López-Obando

Sánchez-Vera

et al. 2022

Preprint

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The Arabidopsis Plant HomeoDomain (PHD) proteins AtMS1 and AtMMD1 provide chromatin-mediated transcriptional regulation essential for tapetum-dependent pollen formation. Such pollen-based male gametogenesis is a derived trait of seed plants. Male gametogenesis in the common ancestors of land plants is instead likely to have been reminiscent of that in extant bryophytes where flagellated sperms are produced by an elaborate gametophyte generation. Still, also bryophytes possess MS1/MMD1-related PHD proteins. We addressed the function of two MS1/MMD1-homologs in the bryophyte model moss Physcomitrium patens by the generation and analysis of reporter and loss-of-function lines. The two genes are together essential for both male and female fertility by providing cell autonomous functions in the gamete-producing inner cells of antheridia and archegonia. They are furthermore expressed in the diploid sporophyte generation suggesting a function during sporogenesis, a process proposed related by descent to pollen formation in angiosperms. We propose that the moss MS1/MMD1-related regulatory network required for completion of male and female gametogenesis and possibly for sporogenesis, represent a heritage from ancestral land plants.

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AtNOT1 Is a Novel Regulator of Gene Expression during Pollen Development

Cited by 13 publications

References 48 publications

Mechanisms and Candidate Genes for Seed and Fruit Set in Grapevine

Mechanisms and Candidate Genes for Seed and Fruit Set in Grapevine

Possible molecular mechanisms of persistent pollen tube growth without de novo transcription

MS1/MMD1homologs in the mossP. patensare required for male and female gametogenesis and likely for sporogenesis

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