Complete extraction of analytes from samples and the homogeneity of analyzed solutions were achieved using ultrasonic treatment (20 minutes). The sensitivity, precision, and accuracy of atomic absorption determination of analytes were improved by using Triton X-100 (4%) and calibration solutions based on iron and manganese acetylacetonates. It was demonstrated that sensitivity increased by 1.7 times for manganese and 1.5 times for iron. The iron and manganese content in medicinal salt mixtures was determined using both atomic absorption and inductively coupled plasma atomic emission methods. The results obtained by these two independent methods were compared, showing that the dispersions are homogeneous and the variation in results is minor, attributed to random error. The accuracy of the atomic absorption results was verified using the standard addition method and by varying the sample weight, confirming that systematic error is negligible. The detection limits established by the atomic absorption method were Cmin=0.009 g/mL for iron (Cmin, lit=0.015 g/mL) and Cmin=0.003 g/mL for manganese (Cmin, lit=0.004 g/mL).