Atomic force microscope-based single cell force spectroscopy of breast cancer cell lines: An approach for evaluating cellular invasion

Omidvar, Ramin; Tafazzoli-Shadpour, Mohammad; Shokrgozar, Mohammad Ali; Rostami, Mostafa

doi:10.1016/j.jbiomech.2014.08.002

Cited by 80 publications

(79 citation statements)

References 31 publications

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“…Thus, due to the differences in microenvironments, cells derived from normal tissues and tumors are intrinsically different in their stiffness, which allows Young's modulus to be a reliable biomarker in the evaluation of metastasis and the motility of cells. In addition, the morphological observations support this opinion experimentally, showing that the cancerous cells usually have decreased stress fibers [25,29,33,67,68], increased cortical actin [25,33], and more lamellipodia [26,65], which benefit the invasion of cells by decreasing adhesion with the substrate and increasing the contraction forces for deformation and protrusion [27,64]. Taking into account that the nuclei are usually softer in cells with higher motility [58] and that the cortex actin network is much softer than the stress fibers [41], it is reasonable to detect a lower Young's modulus in metastatic carcinoma cells.…”

Section: Data Volumementioning

confidence: 57%

“…However, differences in the settings, the application of theoretical models, and the selection of cantilevers greatly affect Young's modulus detected by AFM, which leads to the diversity of Young's moduli in the same cell line characterized by different groups [9,18,20,23,25,26]. For example, there is a 20-fold between Young's moduli of mouse embryonic fibroblast cells NIH-3T3 (1.03 kPa vs. 20 kPa) detected by two different groups [9,20].…”

Section: Methodological Issuesmentioning

confidence: 99%

“…A large difference exists between the studies that assessed the stiffness of cells with modified motility through pharmacological treatment or genetic modification, and cells with higher motility were demonstrated to be softer [8][9][10][16][17][18][19], stiffer [20][21][22][23][24] or unaltered [15] in different studies. Additionally, the values of Young's modulus of the same cell line vary greatly between different investigations [16,25,26], suggesting a great influence of methodological issues on the data obtained by AFM, which will be discussed in the next section.…”

Section: / 28mentioning

confidence: 99%

See 2 more Smart Citations

Cell stiffness determined by atomic force microscopy and its correlation with cell motility

Luo

Kuang

Zhang

et al. 2016

Biochimica et Biophysica Acta (BBA) - General Subjects

180

143

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Section: Data Volumementioning

confidence: 57%

Section: Methodological Issuesmentioning

confidence: 99%

Section: / 28mentioning

confidence: 99%

See 1 more Smart Citation

Cell stiffness determined by atomic force microscopy and its correlation with cell motility

Luo

Kuang

Zhang

et al. 2016

Biochimica et Biophysica Acta (BBA) - General Subjects

180

143

View full text Add to dashboard Cite

“…1,2 In particular, discovering the relation between the cancer cell mechanics and their invasiveness has recently emerged as a research topic of interest. 8 These findings suggest that cellular mechanics strongly correlates with the cancer invasiveness, and Young's modulus might be an indicator of cancer cell invasiveness. 3,4 The smaller Young's modulus and increased cell deformability are major characteristics of cancer cells.…”

Section: Introductionmentioning

confidence: 84%

Modulating cancer cell mechanics and actin cytoskeleton structure by chemical and mechanical stimulations

Azadi

Tafazzoli-Shadpour

Soleimani

et al. 2019

J Biomedical Materials Res

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To date, a myriad of strategies has been suggested for targeting the chemical signaling of cancer cells. Also, biomechanical features are gaining much more attention. These features can be used as biomarkers which influence cancer progression. Current approaches on cancer treatment are mainly focused on changing the biochemical signaling of cancer cells, whereas less attention was devoted to their biomechanical properties. Herein, we propose targeting of cancer cell mechanics through the microenvironmental mechanical and chemical cues. As such, we examined the role of substrate stiffness as well as the effect of epidermal growth factor receptor (EGFR) blockade in the cell mechanics. As a mechanical stimulus, stiff and soft polydimethylsiloxane substrates were utilized, while as a chemical stimulus, EGFR blockade was considered. Thus, breast cancer cell lines, MCF7 and MDA‐MB‐231, were cultured among chemical and mechanical groups. The local elasticity of cancer cells was assessed by atomic force microscopy nanoindentation method. Furthermore, we evaluated the effect of mentioned mechanical and chemical treatments on the morphology, actin cytoskeleton structures, and cancer cell migration abilities. The stiffness and migration ability of cancer cells increased by substrate stiffening while Cetuximab treatment demonstrated an elevation in the elastic modulus of cells followed by a reduction in the migration ability. These findings indicate that cancer cell mechanics is modulated not only by the mechanical cues but also by the chemical ones through EGFR signaling pathway. Overall, our results illustrate that manipulation of cell mechanics allows for the possible modulation of tumor cell migration. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 1569–1581, 2019.

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“…It has been reported, that in case of breast cancer cells the elasticity is inversely proportional to the inter-homocellular adhesion which decreases with metastatic potential (13). Expression of cell adhesion molecules in endothelial cells influences their adhesive properties to bladder cancer cells (14).…”

Section: Introductionmentioning

confidence: 99%

De-adhesion dynamics of melanoma cells from brain endothelial layer

Varga

Domokos

Fazakas

et al. 2018

Biochimica et Biophysica Acta (BBA) - General Subjects

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Metastasis formation is a complex and not entirely understood process. The poorest prognosis and the most feared complications are associated to brain metastases. Melanoma derived brain metastases show the highest prevalence. Due to the lack of classical lymphatic drainage, in the process of brain metastases formation the haematogenous route is of primordial importance. The first and crucial step in this multistep process is the establishment of firm adhesion between the blood travelling melanoma cells and the tightly connected layer of the endothelium, which is the fundamental structure of the blood-brain barrier. This study compares the de-adhesion properties and dynamics of three melanoma cells types (WM35, A2058 and A375) to a confluent layer of brain micro-capillary endothelial cells. Cell type dependent adhesion characteristics are presented, pointing towards the existence of metastatic potential related nanomechanical aspects. Apparent mechanical properties such as elasticity, maximal adhesion force, number, size and distance of individual rupture events showed altered values pointing towards cell type dependent aspects. Our results underline the importance of mechanical details in case of intercellular interactions. Nevertheless, it suggests that in adequate circumstances elastic and adhesive characterizations might be used as biomarkers.

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Atomic force microscope-based single cell force spectroscopy of breast cancer cell lines: An approach for evaluating cellular invasion

Cited by 80 publications

References 31 publications

Cell stiffness determined by atomic force microscopy and its correlation with cell motility

Cell stiffness determined by atomic force microscopy and its correlation with cell motility

Modulating cancer cell mechanics and actin cytoskeleton structure by chemical and mechanical stimulations

De-adhesion dynamics of melanoma cells from brain endothelial layer

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