Atomic force microscope coupled with an optical microscope

Kaneko, Reizo; Oguchi, S.; Hara, Shinji; Matsuda, Ryuichi; Okada, Takao; Ogawa, Haruo; Nakamura, Yasushi

doi:10.1016/0304-3991(92)90480-8

Cited by 26 publications

(4 citation statements)

References 9 publications

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“…There is no conclusion yet on this subject. For some specimens labelled with appropriate fluorophores, such as purified organelles, a light microscope is a welcome addition in helping to place the AFM probe at the right spot (Henderson, 1994;Kaneko et al 1992;Moers et al 1994;van Hulst et al 1993), and to corroborate with the AFM images.…”

Section: Latest Progress In Instrumentationmentioning

confidence: 99%

“…Several groups have combined an AFM with a high power light microscope, so that the specimen, for example purified organelles or isolated cells, can be found and placed under the AFM tip (Henderson, 1994;Kaneko et al 1992;Moers et al 1994;van Hulst et al 1993). The positioning of smaller structures may be aided by fluorescent labels.…”

Section: Combination With Other Microscopesmentioning

confidence: 99%

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Progress in high resolution atomic force microscopy in biology

Shao

Yang

1995

Quart. Rev. Biophys.

156

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The atomic force microscope (AFM) was invented by Binnig, Quate and Gerber less than 10 years ago (Binniget al. 1986). In their first prototype, a piece of goldfoil was used as the cantilever, with a crushed diamond tip mounted at the end. On the back of the cantilever, a tunnelling junction was used to monitor the deflection of the cantilever (the gold-foil) when the specimen was scanned with the tip in contact with the surface. Thus, the surface topography of the specimen was obtained with a resolution critically dependent on the sharpness of the tip provided the deformation of the specimen was not serious. Even with such a crude set-up, they managed to obtain a lateral resolution of ˜ 30 Å and a vertical resolution of better than 1 Å on an amorphous A12O3surface. The operating principle of such an instrument is deceptively simple. However, such an arrangement was inconvenient for routine operations and unsuitable for imaging hydrated specimens, because the tunnelling junction is easily contaminated in air and works poorly in aqueous solutions (Alexanderet al. 1989). As a result, the application of this type of AFM to biological samples was rare (Engel, 1991).

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Section: Latest Progress In Instrumentationmentioning

confidence: 99%

Section: Combination With Other Microscopesmentioning

confidence: 99%

Progress in high resolution atomic force microscopy in biology

Shao

Yang

1995

Quart. Rev. Biophys.

156

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“…14 All observations were performed in air. The ambient temperature was about 25°C and the relative humidity was 40-50%.…”

Section: B Samples and Measurement Conditionmentioning

confidence: 99%

Effect of lubricant coating on tips in atomic force microscopy

Umemura¹

1998

Journal of Vacuum Science &Amp; Technology B: Microelectronics and Nanometer Structures Processing, Measurement, and Phenomena

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“…On the other hand, the ray travels on a curved path from 0 to B in the GRIN medium. The optical path can be numerically calculated by solving 'L((nr)di)=V (nr) (6).…”

Section: Measurement With Interferometrymentioning

confidence: 99%

<title>Precise measurements of refractive index distribution and optical surfaces</title>

Morokuma

1995

SPIE Proceedings

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Described are the two frontier areas of interest, that is, the measurement of refractive index distribution in gradient index glasses and the precise evaluation and measurement of optical surfaces for high precision optics. Scanning total reflection method and interferometric methods are applied to the refractive index measurement with an accuracy of iO to iO. An AFM is found very useful for the evaluation of non-conductive surfaces as well as multilayers for x-ray optics. Methods for absolule measurement of surface profiles are proposed in consideration of the deformation due to gravity and another method for large surfaces.

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Atomic force microscope coupled with an optical microscope

Cited by 26 publications

References 9 publications

Progress in high resolution atomic force microscopy in biology

Progress in high resolution atomic force microscopy in biology

Effect of lubricant coating on tips in atomic force microscopy

<title>Precise measurements of refractive index distribution and optical surfaces</title>

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