Atomic force microscopy: A nanobiotechnology for cellular research

Guan, Guangzhao; Li, Mei; He, Yan

doi:10.26599/ntm.2022.9130004

Cited by 7 publications

(4 citation statements)

References 87 publications

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“…Surface characteristics, such as roughness and topography, have significant effect on the cell response, for example, Ti surface significantly enhances osseointegration [16]. In the literature, biomaterial surfaces are often characterized using different microscopy technologies, such as profilometry, AFM, scanning electron microscopy, and confocal laser scanning microscopy [17][18][19]. Among these technologies, profilometry and AFM can provide both quantitative and quantitative information of the material surfaces.…”

Section: Discussionmentioning

confidence: 99%

“…Apart from the surface roughness measurement, AFM was developed and has been widely used as a method for imaging biomolecules with atomic level resolution under real-time physiological condition. It has been used to investigate the nanomechanical characteristics of cells, tissues, microorganisms, and biological macromolecules like proteins, lipids, mRNA, and DNA [19,[22][23][24]. Profilometer on the other hand is an easy to use and compact optical profiler.…”

Section: Discussionmentioning

confidence: 99%

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Profilometry and atomic force microscopy for surface characterization

Li¹,

Guan²

2023

Nano TransMed

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Aim: This study aims to evaluate and compare the profilometry and atomic force microscopy (AFM) for characterization of biomaterial surfaces. Method: The clinically commonly used titanium (Ti) was used as the specimen. Each of the specimen was prepared by different grits of sandpapers, including 2000, 1000, 800, 600, 400, 220, 180, and 100 grits. An unpolished Ti plate served as the control. Surface characterization of the Ti specimens was examined using profilometry and AFM. Results: Both profilometry and AFM were capable of producing two-dimensional (2D) and three-dimensional (3D) topography. The scanning speed of profilometry (12 ± 5 s/image) was faster than that of AFM (250 ± 50 s/image) (p < 0.01). The resolution of AFM was relatively higher than profilometry. AFM produced more precise value, especially at nano-scale. When the Ti surface roughness was less than 0.2 μm, the results of surface roughness measured by profilometry and AFM were similar (mean difference = 0.01 ± 0.03, p = 0.81). When the Ti surface roughness was more than 0.3 μm, the surface roughness measured by profilometry was slightly higher than that by AFM (mean difference = 0.43 ± 0.15, p = 0.04). Conclusion: Profilometry and AFM are both useful techniques for the characterization of biomaterial surfaces. Profilometry scanned faster than the AFM but produced less detailed surface topography. Both technologies provided similar measurement when the roughness was less than 0.2 μm. When the Ti surface roughness was more than 0.3 μm, the surface roughness measured by profilometry was slightly higher than that by AFM.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Profilometry and atomic force microscopy for surface characterization

Li¹,

Guan²

2023

Nano TransMed

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“…AFM generates high-resolution images of the surface of biomolecules, membranes, cells, and tissues, and can also probe their mechanical, chemical, electrostatic, and biological properties [ 215 ]. The AFM basic principles, the modes of imaging of biointerfaces, molecular and force spectroscopy, and the advantages and limitations of AFM-related techniques have been reviewed in [ 215 , 216 ]. The main capability of AFM is to detect the weak forces acting between a very sharp tip (called a probe) and the sample under examination.…”

Section: Atomic Force Microscopy: Morphometric and Nanomechanical Par...mentioning

confidence: 99%

“…The probe is attached to a flexible cantilever, which deforms as a result of the forces of attraction and repulsion between the tip and the surface (Figure 1). related techniques have been reviewed in [215,216]. The main capability of AFM is to detect the weak forces acting between a very sharp tip (called a probe) and the sample under examination.…”

Section: Atomic Force Microscopy As a Diagnostic Toolmentioning

confidence: 99%

Morphometric and Nanomechanical Screening of Peripheral Blood Cells with Atomic Force Microscopy for Label-Free Assessment of Alzheimer’s Disease, Parkinson’s Disease, and Amyotrophic Lateral Sclerosis

Taneva,

Todinova,

Andreeva

2023

IJMS

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Neurodegenerative disorders (NDDs) are complex, multifactorial disorders with significant social and economic impact in today’s society. NDDs are predicted to become the second-most common cause of death in the next few decades due to an increase in life expectancy but also to a lack of early diagnosis and mainly symptomatic treatment. Despite recent advances in diagnostic and therapeutic methods, there are yet no reliable biomarkers identifying the complex pathways contributing to these pathologies. The development of new approaches for early diagnosis and new therapies, together with the identification of non-invasive and more cost-effective diagnostic biomarkers, is one of the main trends in NDD biomedical research. Here we summarize data on peripheral biomarkers, biofluids (cerebrospinal fluid and blood plasma), and peripheral blood cells (platelets (PLTs) and red blood cells (RBCs)), reported so far for the three most common NDDs—Alzheimer’s disease (AD), Parkinson’s disease (PD), and amyotrophic lateral sclerosis (ALS). PLTs and RBCs, beyond their primary physiological functions, are increasingly recognized as valuable sources of biomarkers for NDDs. Special attention is given to the morphological and nanomechanical signatures of PLTs and RBCs as biophysical markers for the three pathologies. Modifications of the surface nanostructure and morphometric and nanomechanical signatures of PLTs and RBCs from patients with AD, PD, and ALS have been revealed by atomic force microscopy (AFM). AFM is currently experiencing rapid and widespread adoption in biomedicine and clinical medicine, in particular for early diagnostics of various medical conditions. AFM is a unique instrument without an analog, allowing the generation of three-dimensional cell images with extremely high spatial resolution at near-atomic scale, which are complemented by insights into the mechanical properties of cells and subcellular structures. Data demonstrate that AFM can distinguish between the three pathologies and the normal, healthy state. The specific PLT and RBC signatures can serve as biomarkers in combination with the currently used diagnostic tools. We highlight the strong correlation of the morphological and nanomechanical signatures between RBCs and PLTs in PD, ALS, and AD.

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Advances in posterity of visualization in paradigm of nano‐level ultra‐structures for nano–bio interaction studies

Ghosh,

Gupta,

Jena

et al. 2024

VIEW

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The progression in contemporary scientific field is facilitated by a multitude of sophisticated and cutting‐edge methodologies that are employed for various research purposes. Among these methodologies, microscopy stands out as a fundamental and essential technique utilized in scientific investigations. Moreover, due to the continuous evolution and enhancement of microscopic methodologies, nanotechnology has reached a highly developed stage within modern scientific realm, particularly renowned for its wide‐ranging applications in the fields of biomedicine and environmental science. When it comes to conducting comprehensive and in‐depth experimental analyses to explore the nanotechnological aspects relevant to biological applications, the concept of nano–biological interaction emerges as the focal point of any research initiative. Nonetheless, this particular study necessitates a meticulous approach toward imaging and visualization at diverse magnification levels to ensure accurate observations and interpretations. It is widely acknowledged that modern microscopy has emerged as a sophisticated and invaluable instrument in this regard. This review aims to provide a comprehensive discussion on the progress made in microscopic techniques specifically tailored for visualizing the interactions between nanostructures and biological entities, thereby facilitating the exploration of the practical applications of nanotechnology in the realm of biological sciences.

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Atomic force microscopy: A nanobiotechnology for cellular research

Cited by 7 publications

References 87 publications

Profilometry and atomic force microscopy for surface characterization

Profilometry and atomic force microscopy for surface characterization

Morphometric and Nanomechanical Screening of Peripheral Blood Cells with Atomic Force Microscopy for Label-Free Assessment of Alzheimer’s Disease, Parkinson’s Disease, and Amyotrophic Lateral Sclerosis

Advances in posterity of visualization in paradigm of nano‐level ultra‐structures for nano–bio interaction studies

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