Atomic force microscopy and other scanning probe microscopy methods to study nanoscale domains in model lipid membranes

Robinson, Morgan; Filice, Carina T.; McRae, Danielle M.; Leonenko, Zoya

doi:10.1080/23746149.2023.2197623

Cited by 3 publications

(3 citation statements)

References 180 publications

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“…Models made to mimic cell membranes can be of varying complexity, from a single lipid to a complex model of five or more lipids [22,109]. These models can exhibit phase separation, forming micro-and nanoscale domains, which can be critical for membrane-protein interactions [110]. Experimentally, these are formed via the vesicle fusion method, or by Langmuir Blodgett deposition on flat surfaces to form supported lipid bilayers (SLBs) [18,20].…”

Section: Applications Of Scanning Probe Microscopy In Cellular Membra...mentioning

confidence: 99%

Applications of scanning probe microscopy in neuroscience research

McRae,

Leonenko

2024

J. Phys. Mater.

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Scanning probe microscopy techniques allow for label-free high-resolution imaging of cells, tissues, and biomolecules in physiologically relevant conditions. These techniques include atomic force microscopy (AFM), atomic force spectroscopy, and Kelvin probe force microscopy (KPFM), which enable high resolution imaging, nanomanipulation and measurement of the mechanoelastic properties of neuronal cells, as well as scanning ion conductance microscopy (SICM), which combines electrophysiology and imaging in living cells. The combination of scanning probe techniques with optical spectroscopy, such as with AFM-IR and tip-enhanced Raman spectroscopy (TERS), allows for the measurement of topographical maps along with chemical identity, enabled by spectroscopy. In this work, we review applications of these techniques to neuroscience research, where they have been used to study the morphology and mechanoelastic properties of neuronal cells and brain tissues, and to study changes in these as a result of chemical or physical stimuli. Cellular membrane models are widely used to investigate the interaction of the neuronal cell membrane with proteins associated with various neurological disorders, where scanning probe microscopy and associated techniques provide significant improvement in the understanding of these processes on a cellular and molecular level.

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Section: Applications Of Scanning Probe Microscopy In Cellular Membra...mentioning

confidence: 99%

Applications of scanning probe microscopy in neuroscience research

McRae,

Leonenko

2024

J. Phys. Mater.

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“…The cell membrane is composed of various lipids and proteins [ 91 , 92 , 93 ] and acts as a protective barrier and gatekeeper [ 94 , 95 ]. The cell membrane is a representative semi-permeable membrane that allows certain ions or molecules to pass through it via osmosis but not others [ 96 ].…”

Section: Roles Of Osmotic Pressurementioning

confidence: 99%

Osmotic Pressure and Its Biological Implications

Zheng,

Li,

Shao

et al. 2024

IJMS

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Gaining insight into osmotic pressure and its biological implications is pivotal for revealing mechanisms underlying numerous fundamental biological processes across scales and will contribute to the biomedical and pharmaceutical fields. This review aims to provide an overview of the current understanding, focusing on two central issues: (i) how to determine theoretically osmotic pressure and (ii) how osmotic pressure affects important biological activities. More specifically, we discuss the representative theoretical equations and models for different solutions, emphasizing their applicability and limitations, and summarize the effect of osmotic pressure on lipid phase separation, cell division, and differentiation, focusing on the mechanisms underlying the osmotic pressure dependence of these biological processes. We highlight that new theory of osmotic pressure applicable for all experimentally feasible temperatures and solute concentrations needs to be developed, and further studies regarding the role of osmotic pressure in other biological processes should also be carried out to improve our comprehensive and in-depth understanding. Moreover, we point out the importance and challenges of developing techniques for the in vivo measurement of osmotic pressure.

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“…In the last decade many techniques have been developed and utilised to measure mechanical properties of living biological materials, which enable in situ measurements (Elsayad et al, 2016; Farokh Payam & Passian, 2024; Kitamura et al, 2023; Michels et al, 2020; Robinson et al, 2023; Seelbinder et al, 2024; P.-H. Wu et al, 2018). Cell microenvironment-sensing dyes based on fluorescence lifetime (FLIM) signals have proven their robustness, independence from concentration, precision in revealing cellular characteristics, such as pH, intrinsically disordered proteins status, redox status, hormone concentrations, and biomechanical parameters, such as viscosity and ionic strength as well (Colin et al, 2022; Cuevas-Velazquez et al, 2021; Kitamura et al, 2023; Koveal et al, 2020; Michels et al, 2020; Miller et al, 2020; Steinbeck et al, 2020; van der Linden et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

The viscoelastic properties ofNicotiana tabacumBY-2 suspension cell lines adapted to high osmolarity

Skrzypczak,

Pochylski,

Rapp

et al. 2024

Preprint

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Plant cells have to maintain the cellular microenvironment within a certain range of its properties to stay alive and grow. The functioning of the cell requires a continuous exchange of mass and energy with an environment. Such system openness could lead to perturbation in cellular microenvironment and disorder in molecular processes. Hyperosmolarity significantly stresses cells, induces ROS, promotes water efflux, and decreases turgor, optionally up to plasmolysis and a cell's shrinkage. However, in time upon exposure to osmotic stress a cellular adaptation could be developed. How the biomechanical balance of the cell is established and maintained in an environment of osmotic stress remains a nurturing question. We show that changes of the viscoelastic properties of protoplasts are element of such adaptation. We used Brillouin light scattering (BLS) and BODIPY-based fluorescence molecular rotors to study Nicotiana tabacum suspension BY-2 cells adapted to high concentrations of NaCl and mannitol. We revealed an increased molecular crowd and viscoelasticity in adapted BY-2 cells, as well as an altered plasma membrane tension. Importantly, viscosity-related changes imposed on adapted BY-2 cells by salt stress were smaller than those observed for control cells. Cellular microenvironments were elucidated in on the background of their own environments, what revealed that alterations of cytoplasm and vacuoles viscoelasticity in adapted cells has also a relative dimension. This work provides the first semi-quantitative data, to our knowledge, for viscoelastic cellular changes in plant cells adapted to growth under salt and osmotic stress conditions and exposed to subsequent short-term stress. Applied methods provide evidence that adaptation to hyperosmotic stress leads to different ratio in the protoplast and environment qualities that helps to maintain cell integrity. Many challenges remain in the field of plant mechanobiology, to which our approach could provide valuable insight into plant cell responses to the environment and the further development of useful methods.

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Atomic force microscopy and other scanning probe microscopy methods to study nanoscale domains in model lipid membranes

Cited by 3 publications

References 180 publications

Applications of scanning probe microscopy in neuroscience research

Applications of scanning probe microscopy in neuroscience research

Osmotic Pressure and Its Biological Implications

The viscoelastic properties ofNicotiana tabacumBY-2 suspension cell lines adapted to high osmolarity

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