Atomic force microscopy detects differences in the surface brush of normal and cancerous cells

Iyer, Swaminathan Padmanabhan; Gaikwad, Ravi; Subba-Rao, Venkatesh; Woodworth, Craig D.; Sokolov, Igor

doi:10.1038/nnano.2009.77

Cited by 320 publications

(408 citation statements)

References 27 publications

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“…The potential specificity of nanoparticles to attach to diseased cells as against healthy cells [136] offers selectivity, and the use of ROS or RNS carried by nanoparticles as the main agent against either cancer cells or pathogens reduces the reliance on toxic materials (e.g. silver).…”

Section: Synergy In Reaction Chemistrmentioning

confidence: 99%

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Plasmas meet nanoparticles—where synergies can advance the frontier of medicine

Kong¹,

Keidar²,

Ostrikov³

2011

J. Phys. D: Appl. Phys.

109

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To cite this version:M G Kong, M Keidar, K Ostrikov. Plasmas meet nanoparticleswhere synergies can advance the frontier of medicine. Journal of Physics D: Applied Physics, IOP Publishing, 2011, 44 (17) AbstractNanoparticles and low-temperature plasmas have been developed, independently and often along different routes, to tackle the same set of challenges in biomedicine. There are intriguing similarities and contrasts in their interactions with cells and living tissues, and these are reflected directly in the characteristics and scope of their intended therapeutic solutions, in particular their chemical reactivity, selectivity against pathogens and cancer cells, safety to healthy cells and tissues, and targeted delivery to diseased tissues. Time has come to ask the inevitable question of possible plasma-nanoparticle synergy and the related benefits to the development of effective, selective, and safe therapies for modern medicine. This perspective article offers a detailed review of the strengths and weakenesses of nanomedicine and plasma medicine as a stand-alone technology, and then provides a critical analysis of some of the major opportunities enabled by synergising the nanotechnology and the plasma technology. It is shown that the plasma-nanoparticle synergy is best captured through the plasma nanotechnology and its benefits for medicine are highly promising.

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Section: Synergy In Reaction Chemistrmentioning

confidence: 99%

“…It is known that nanoparticles may preferentially deposit near cancer cells rather than healthy cells [136]. Therefore a combined use of plasmas and nanoparticles is likely to enhance the directionality of the propagation of both plasma species and nanoparticles towards diseased sites within a tissue.…”

Section: Synergy For Enhanced Penetration and Selectivitymentioning

confidence: 99%

Plasmas meet nanoparticles—where synergies can advance the frontier of medicine

Kong¹,

Keidar²,

Ostrikov³

2011

J. Phys. D: Appl. Phys.

109

View full text Add to dashboard Cite

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“…When a normal cell transforms into a cancerous cell, its structures and functions change considerably (Hanahan and Weinberg, 2011). For example, cellular surface brush layers (mainly consisting of microvilli, microridges and cilia) between normal cells and cancerous cells differ significantly (Iyer et al, 2009). Here, we can observe the significant differences between normal breast cells and cancerous breast cells in several aspects, including cellular growth, cellular shape, cellular edge and cellular geometric features.…”

Section: Rusults and Discussionmentioning

confidence: 99%

Atomic force microscopy studies on cellular elastic and viscoelastic properties

Liu

et al. 2017

Sci. China Life Sci.

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In this work, a method based on atomic force microscopy (AFM) approach-reside-retract experiments was established to simultaneously quantify the elastic and viscoelastic properties of single cells. First, the elastic and viscoelastic properties of normal breast cells and cancerous breast cells were measured, showing significant differences in Young's modulus and relaxation times between normal and cancerous breast cells. Remarkable differences in cellular topography between normal and cancerous breast cells were also revealed by AFM imaging. Next, the elastic and viscoelasitc properties of three other types of cell lines and primary normal B lymphocytes were measured; results demonstrated the potential of cellular viscoelastic properties in complementing cellular Young's modulus for discerning different states of cells. This research provides a novel way to quantify the mechanical properties of cells by AFM, which allows investigation of the biomechanical behaviors of single cells from multiple aspects.

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“…40 Moreover, it has been demonstrated that it is possible to detect length and grafting density of the brush layer on living cells, 65,66 or even to distinguish between cancerous and healthy cells' brushes, 67 thanks to the sensitivity of micrometric spherical probes provided by their wider surface area.…”

Section: Colloidal Spherical Probesmentioning

confidence: 99%

Nanomechanical and topographical imaging of living cells by atomic force microscopy with colloidal probes

Puricelli

Galluzzi

Schulte

et al. 2015

Review of Scientific Instruments

161

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Atomic Force Microscopy (AFM) has a great potential as a tool to characterize mechanical and morphological properties of living cells; these properties have been shown to correlate with cells' fate and patho-physiological state in view of the development of novel early-diagnostic strategies. Although several reports have described experimental and technical approaches for the characterization of cellular elasticity by means of AFM, a robust and commonly accepted methodology is still lacking.Here we show that micrometric spherical probes (also known as colloidal probes) are well suited for performing a combined topographic and mechanical analysis of living cells, with spatial resolution suitable for a complete and accurate mapping of cell morphological and elastic properties, and superior reliability and accuracy in the mechanical measurements with respect to conventional and widely used sharp AFM tips. We address a number of issues concerning the nanomechanical analysis, including the applicability of contact mechanical models and the impact of a constrained contact geometry on the measured Young's modulus (the finite-thickness effect). We have tested our protocol by imaging living PC12 and MDA-MB-231 cells, in order to demonstrate the importance of the correction of the finite-thickness effect and the change in Young's modulus induced by the action of a cytoskeleton-targeting drug.

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Atomic force microscopy detects differences in the surface brush of normal and cancerous cells

Cited by 320 publications

References 27 publications

Plasmas meet nanoparticles—where synergies can advance the frontier of medicine

Plasmas meet nanoparticles—where synergies can advance the frontier of medicine

Atomic force microscopy studies on cellular elastic and viscoelastic properties

Nanomechanical and topographical imaging of living cells by atomic force microscopy with colloidal probes

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