Atomic force microscopy for imaging and nanomechanical characterisation of live nematode epicuticle: A comparative Caenorhabditis elegans and Turbatrix aceti study

Akhatova, Farida; Fakhrullina, Gölnur; Khakimova, Elvira; Fakhrullin, Rawil

doi:10.1016/j.ultramic.2018.07.008

Cited by 21 publications

(16 citation statements)

References 33 publications

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“…Atomic force microscopy (AFM) images were obtained using a Dimension Icon microscope (Bruker, Elmsford, NY, USA) equipped with Scan Asyst-Air (Bruker) probes (tip radius 2 nm, nominal length 115 µm, radius 2 nm, spring constant 0.4 nm −1 ) for surface topography. Raw data were analyzed with Nanoscope Analysis software (Bruker, v 1.7, Elmsford, NY, USA), as described in a previous report [ 23 ]. Hair segments were attached to regular microscopy slides with an adhesive type and then imaged in the air under ambient temperature and humidity in Peak Force Tapping QNM mode.…”

Section: Methodsmentioning

confidence: 99%

Clay Nanotube Immobilization on Animal Hair for Sustained Anti-Lice Protection

et al. 2021

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Topical administration of drugs is required for the treatment of parasitic diseases and insect infestations; therefore, fabrication of nanoscale drug carriers for effective insecticide topical delivery is needed. Here we report the enhanced immobilization of halloysite tubule nanoclay onto semiaquatic capybaras which have hydrophobic hair surfaces as compared to their close relatives, land-dwelling guinea pigs, and other agricultural livestock. The hair surface of mammals varies in hydrophobicity having a cortex surrounded by cuticles. Spontaneous 1–2 µm thick halloysite hair coverages on the semi-aquatic rodent capybara, non-aquatic rodent guinea pig, and farm goats were compared. The best coating was found for capybara due to the elevated 5 wt% wax content. As a result, we suggest hair pretreatment with diluted wax for enhanced nanoclay adsorption. The formation of a stable goat hair coverage with a 2–3 µm halloysite layer loaded with permethrin insecticide allowed for long-lasting anti-parasitic protection, enduring multiple rain wettings and washings. We expect that our technology will find applications in animal parasitosis protection and may be extended to prolonged human anti-lice treatment.

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Section: Methodsmentioning

confidence: 99%

Clay Nanotube Immobilization on Animal Hair for Sustained Anti-Lice Protection

et al. 2021

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“…AFM images were obtained using a Dimension Icon instrument (Bruker, Billerica, MA, USA) operating in PeakForce Tapping mode [ 24 ]. To obtain the images, ScanAsyst-Air (Bruker, USA) probes (nominal length 115 μm, tip of radius 2 nm, spring stiffness 0.4 N m −1 ) was used throughout this study.…”

Section: Methodsmentioning

confidence: 99%

Revisiting the Cytotoxicity of Cationic Polyelectrolytes as a Principal Component in Layer-by-Layer Assembly Fabrication

et al. 2021

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Polycations are an essential part of layer-by-layer (LbL)-assembled drug delivery systems, especially for gene delivery. In addition, they are used for other related applications, such as cell surface engineering. As a result, an assessment of the cytotoxicity of polycations and elucidation of the mechanisms of polycation toxicity is of paramount importance. In this study, we examined in detail the effects of a variety of water-soluble, positively charged synthetic polyelectrolytes on in vitro cytotoxicity, cell and nucleus morphology, and monolayer expansion changes. We have ranked the most popular cationic polyelectrolytes from the safest to the most toxic in relation to cell cultures. 3D cellular cluster formation was disturbed by addition of polyelectrolytes in most cases in a dose-dependent manner. Atomic force microscopy allowed us to visualize in detail the structures of the polyelectrolyte–DNA complexes formed due to electrostatic interactions. Our results indicate a relationship between the structure of the polyelectrolytes and their toxicity, which is necessary for optimization of drug and gene delivery systems.

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“…The images were obtained using ScanAsyst-air probes (Bruker, Billerica, MA, USA) (nominal length 115 µm, tip radius 2 nm, nominal spring constant 0.4 N m −1 ). The data were processed with the Nanoscope Analysis v.3.0 software (Bruker, Billerica, MA, USA) [ 62 ]. The adhesion images were not edited for reliable results.…”

Section: Methodsmentioning

confidence: 99%

Pharmaceuticals Removal by Adsorption with Montmorillonite Nanoclay

Kryuchkova

Batasheva

Akhatova

et al. 2021

IJMS

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The problem of purifying domestic and hospital wastewater from pharmaceutical compounds is becoming more and more urgent every year, because of the continuous accumulation of chemical pollutants in the environment and the limited availability of freshwater resources. Clay adsorbents have been repeatedly proposed as adsorbents for treatment purposes, but natural clays are hydrophilic and can be inefficient for catching hydrophobic pharmaceuticals. In this paper, a comparison of adsorption properties of pristine montmorillonite (MMT) and montmorillonite modified with stearyl trimethyl ammonium (hydrophobic MMT-STA) towards carbamazepine, ibuprofen, and paracetamol pharmaceuticals was performed. The efficiency of adsorption was investigated under varying solution pH, temperature, contact time, initial concentration of pharmaceuticals, and adsorbate/adsorbent mass ratio. MMT-STA was better than pristine MMT at removing all the pharmaceuticals studied. The adsorption capacity of hydrophobic montmorillonite to pharmaceuticals decreased in the following order: carbamazepine (97%) > ibuprofen (95%) > paracetamol (63–67%). Adsorption isotherms were best described by Freundlich model. Within the pharmaceutical concentration range of 10–50 µg/mL, the most optimal mass ratio of adsorbates to adsorbents was 1:300, pH 6, and a temperature of 25 °C. Thus, MMT-STA could be used as an efficient adsorbent for deconta×ating water of carbamazepine, ibuprofen, and paracetamol.

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Atomic force microscopy for imaging and nanomechanical characterisation of live nematode epicuticle: A comparative Caenorhabditis elegans and Turbatrix aceti study

Cited by 21 publications

References 33 publications

Clay Nanotube Immobilization on Animal Hair for Sustained Anti-Lice Protection

Clay Nanotube Immobilization on Animal Hair for Sustained Anti-Lice Protection

Revisiting the Cytotoxicity of Cationic Polyelectrolytes as a Principal Component in Layer-by-Layer Assembly Fabrication

Pharmaceuticals Removal by Adsorption with Montmorillonite Nanoclay

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