Atomic Force Microscopy Imaging of the Human Trigeminal Ganglion

Melling, Mahmoud; Hochmeister, Sonja; Blumer, Roland; Schilcher, Kurt; Mostler, Sascha; Behnam, Mark; Wilde, J; Karimian-Teherani, Daniela

doi:10.1006/nimg.2001.0924

Cited by 23 publications

(14 citation statements)

References 15 publications

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“…(ii) AFM is applicable to cells of various sizes, whereas EM cryotomography is currently limited to small cells [2]. AFM allows one to observe structures of various sizes, ranging from large trigeminal ganglion [18] or mouse ES cells (this study) to small nuclei/inner structures [20]. (iii) Serial sections of optional thickness can be visualized using AFM technique.…”

Section: Discussionmentioning

confidence: 99%

“…AFM is also used to compare different material-embedded sections [9] and sections created using various knives [16]. Furthermore, AFM has been employed to image thin-sectioned skeletal muscle [17], semi-thin-sectioned human trigeminal ganglion [18], human oculomotor nerve [19] and epithelial cells of rat kidney [20]. The capacity of AFM to display clear inner structures of cells and/or tissues raises the possibility to visualize nanostructures on serial thin sections of a single cell as well as to perform the 3-D reconstruction of single cells or their inner structures.…”

Section: Introductionmentioning

confidence: 99%

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Atomic force microscopy imaging and 3-D reconstructions of serial thin sections of a single cell and its interior structures

et al. 2005

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The thin sectioning has been widely applied in electron microscopy (EM), and successfully used for an in situ observation of inner ultrastructure of cells. This powerful technique has recently been extended to the research field of atomic force microscopy (AFM). However, there have been no reports describing AFM imaging of serial thin sections and three-dimensional (3-D) reconstruction of cells and their inner structures. In the present study, we used AFM to scan serial thin sections approximately 60nm thick of a mouse embryonic stem (ES) cell, and to observe the in situ inner ultrastructure including cell membrane, cytoplasm, mitochondria, nucleus membrane, and linear chromatin. The high-magnification AFM imaging of single mitochondria clearly demonstrated the outer membrane, inner boundary membrane and cristal membrane of mitochondria in the cellular compartment. Importantly, AFM imaging on six serial thin sections of a single mouse ES cell showed that mitochondria underwent sequential changes in the number, morphology and distribution. These nanoscale images allowed us to perform 3-D surface reconstruction of interested interior structures in cells. Based on the serial in situ images, 3-D models of morphological characteristics, numbers and distributions of interior structures of the single ES cells were validated and reconstructed. Our results suggest that the combined AFM and serial-thin-section technique is useful for the nanoscale imaging and 3-D reconstruction of single cells and their inner structures. This technique may facilitate studies of proliferating and differentiating stages of stem cells or somatic cells at a nanoscale.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Atomic force microscopy imaging and 3-D reconstructions of serial thin sections of a single cell and its interior structures

et al. 2005

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“…Nucleoli can be visualized in nuclei in whole cells using atomic force microscopy, suggesting that they are stiffer than the surrounding nucleoplasm. 62 Similarly, nucleoli appear as fluid structures which deform cohesively in cells which are deformed by micropipette aspiration, and they show permanent deformation under high stress. 59 Although the importance of nucleolar stiffness is unknown, the compact nucleolar structure maintains its shape during short-term mechanical stress and can act as fiducial markers within the nucleoplasm to study subnuclear deformations.…”

Section: Nuclear Bodies and Intranuclear Structuresmentioning

confidence: 99%

Nuclear Shape, Mechanics, and Mechanotransduction

Dahl

Ribeiro

Lammerding

2008

Circulation Research

623

578

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Abstract-In eukaryotic cells, the nucleus contains the genome and is the site of transcriptional regulation. The nucleus is the largest and stiffest organelle and is exposed to mechanical forces transmitted through the cytoskeleton from outside the cell and from force generation within the cell. Here, we discuss the effect of intra-and extracellular forces on nuclear shape and structure and how these force-induced changes could be implicated in nuclear mechanotransduction, ie, force-induced changes in cell signaling and gene transcription. We review mechanical studies of the nucleus and nuclear structural proteins, such as lamins. Dramatic changes in nuclear shape, organization, and stiffness are seen in cells where lamin proteins are mutated or absent, as in genetically engineered mice, RNA interference studies, or human disease. We examine the different mechanical pathways from the force-responsive cytoskeleton to the nucleus. We also highlight studies that link changes in nuclear shape with cell function during developmental, physiological, and pathological modifications. Together, these studies suggest that the nucleus itself may play an important role in the response of the cell to force. (Circ Res. 2008;102:1307-1318.)

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“…In the area of neuroscience, the application of AFM to neurons has been limited (Ricci et al, 2004) and has concentrated on internal organelles (Parpura et al, 1993;Lal et al, 1995) or nanoscale features of the surface, including gap junctions, ionic channels, and focal adhesion points (Jena, 1997). Others have used AFM technology to image neurons in the fixed state (Tojima et al, 2000;Weissmuller et al, 2000;Melling et al, 2001). However, the overall architecture of living neurons at high resolution has not been thoroughly evaluated with this technology until the first report in this series (McNally and Borgens, 2004).…”

Section: Introductionmentioning

confidence: 99%

Comparative three-dimensional imaging of living neurons with confocal and atomic force microscopy

McNally

Rajwa

Sturgis

et al. 2005

Journal of Neuroscience Methods

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Comparative three-dimensional imaging of living neurons with confocal and atomic force microscopy" (2005 AbstractAtomic force microscopy applications extend across a number of fields; however, limitations have reduced its effectiveness in live cell analysis. This report discusses the use of AFM to evaluate the three-dimensional (3-D) architecture of living chick dorsal root ganglia and sympathetic ganglia. These data sets were compared to similar images acquired with confocal laser scanning microscopy of identical cells. For this comparison we made use of visualization techniques which were applicable to both sets of data and identified several issues when coupling these technologies. These direct comparisons offer quantitative validation and confirmation of the character of novel images acquired by AFM. This paper is one in a series emphasizing various new applications of AFM in neurobiology.

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Atomic Force Microscopy Imaging of the Human Trigeminal Ganglion

Cited by 23 publications

References 15 publications

Atomic force microscopy imaging and 3-D reconstructions of serial thin sections of a single cell and its interior structures

Atomic force microscopy imaging and 3-D reconstructions of serial thin sections of a single cell and its interior structures

Nuclear Shape, Mechanics, and Mechanotransduction

Comparative three-dimensional imaging of living neurons with confocal and atomic force microscopy

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