Atomic force microscopy in biomaterials surface science

Variola, Fabio

doi:10.1039/c4cp04427d

Cited by 63 publications

(73 citation statements)

References 123 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In contrast, we previously reported the fabrication of hydrogels that allow much simpler harvest of the cell sheet and direct application to the target. 22,24,27 The current study was designed to confirm feasibility of cell sheet harvest with aligned myoblasts using the previously developed hydrogels and to further investigate how ECM assembly and myogenic differentiation can be controlled on the resulting cell sheet.…”

Section: Discussionmentioning

confidence: 99%

“…As shown in Figure S1, the size of the patterns on the PDMS mold was not precisely matched to the original dimensions of 20 and 80 m, which might be due to a deflection effect generated by the attractive force between the tip of the cantilever and materials in atomic force microscopy (AFM). 27 Nevertheless, the hydrogels were imprinted well with the PDMS molds, as shown in Figure 1(a). Our thermoresponsive polymer is cross-linked by a pair of initiators, HRP and H 2 O 2 , in pre-solution.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Fabrication of cell sheets with anisotropically aligned myotubes using thermally expandable micropatterned hydrogels

et al. 2016

View full text Add to dashboard Cite

Although many efforts have been made to engineer cell sheets with anisotropic patterns, current techniques still exhibit several shortcomings including uncontrolled harvest time and deformation of pattern by contraction. In this study, we report a simple method to harvest a cell sheet with a striated structure of extracellular matrix (ECM) and myoblasts using a thermosensitive hydrogel micropatterned with different sizes (25 and 80 μm). The hydrogel supported the formation of a confluent monolayer of myoblasts with aligned morphology. When the temperature was reduced from 37 to 4 o C, the size of the hydrogel increased by approximately 1.2-fold within 10 min. In response to this change, a cell sheet was harvested and could be transferred to the desirable substrate through conformal contact between the hydrogel and target. We further examined the effect of pattern size on alignment of ECM/ cell assembly by fast Fourier transform (FFT) analysis of fluorescently stained stress fibers and ECM proteins within the harvested cell sheet. The cell sheet maintained alignment of confluent myoblasts after being harvested to the substrate. In addition, the topologic patterns also promoted formation of aligned myotube on the harvested cell sheet, which was important for muscle tissue regeneration. However, the pattern size appeared to have no influence on alignment of cells on the cell sheet. Finally, a bi-layered structure was fabricated in which each layer was stacked with aligned direction on the cell sheet being perpendicularly assembled. Collectively, our platform could be used for rapid harvest of cell sheets with aligned architecture of ECM/cell, which can be applied to fabrication of welldefined 3D tissue for tissue engineering.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Fabrication of cell sheets with anisotropically aligned myotubes using thermally expandable micropatterned hydrogels

et al. 2016

View full text Add to dashboard Cite

show abstract

“…The first scope of TE is to assemble functional constructs or scaffolds able to restore, maintain, and improve injured or damaged tissues. In this context, it is crucial to investigate specific interactions between single cells and extracellular matrix, into micro/nanostructured scaffolds made of natural or synthetic polymers, under specific stimuli exerted by surrounding biological environment at micro and nanometric scale [9]. …”

Section: Background: Advantages and Limitationsmentioning

confidence: 99%

“…One of the most crucial features of AFM in nanoscience is referred to its capability to characterize the mechanical properties of scaffolds, polymers, proteins, cells and bacteria [9]. In particular, the ability to quantify viscoelasticity of any natural and synthetic polymers is providing new important information for biomedical studies addressed to explore the relationships between the mechanical behaviour and physical and/or chemical properties.…”

Section: Working Principles and Basic Toolsmentioning

confidence: 99%

Atomic Force Microscopy: A Powerful Tool to Address Scaffold Design in Tissue Engineering

Marrese

Guarino

Ambrosio

2017

JFB

108

View full text Add to dashboard Cite

Functional polymers currently represent a basic component of a large range of biological and biomedical applications including molecular release, tissue engineering, bio-sensing and medical imaging. Advancements in these fields are driven by the use of a wide set of biodegradable polymers with controlled physical and bio-interactive properties. In this context, microscopy techniques such as Atomic Force Microscopy (AFM) are emerging as fundamental tools to deeply investigate morphology and structural properties at micro and sub-micrometric scale, in order to evaluate the in time relationship between physicochemical properties of biomaterials and biological response. In particular, AFM is not only a mere tool for screening surface topography, but may offer a significant contribution to understand surface and interface properties, thus concurring to the optimization of biomaterials performance, processes, physical and chemical properties at the micro and nanoscale. This is possible by capitalizing the recent discoveries in nanotechnologies applied to soft matter such as atomic force spectroscopy to measure surface forces through force curves. By tip-sample local interactions, several information can be collected such as elasticity, viscoelasticity, surface charge densities and wettability. This paper overviews recent developments in AFM technology and imaging techniques by remarking differences in operational modes, the implementation of advanced tools and their current application in biomaterials science, in terms of characterization of polymeric devices in different forms (i.e., fibres, films or particles).

show abstract

“…The fact that nanotopographical surfaces influence charge density and electric field strength, quantitative characterization of surface morphology is thus very important. To date, AFM has been employed progressively for exploring the surface morphological features especially in the research area of surface engineering [1,9].…”

Section: Introductionmentioning

confidence: 99%

Nanostructural Characterization of Glutathione-S-Transferase Immobilizing Chitosan Modified Screen Printed Carbon Electrode by Atomic Force Microscopy

Wongkaew¹

2018

GEOMATE

View full text Add to dashboard Cite

Immobilization of a bio-recognition element to the surface of a functional working electrode is fundamental for effective biosensor development. In this study, the enzyme glutathione-s-transferase (GST) that constitutes a protein superfamily involving various distinct chemical transformations was introduces as a versatile tool for the sensing of environmental toxicants. Functional electrode surface was made by selfassembly of a great bioscaffold chitosan onto screen-printed carbon electrode surface concerning to its excellent covalent bonding binding of biomolecules. To enhance the enzyme proximity, glutaraldehyde was employed as an assisting bifunctional cross-linker. The self assembled chitosan layer and the GST immobilizing nanostructural features were explored by morphological imaging and several quantitative analyses such as surface grain size and distribution, power spectrum density (PSD) algorithm, fractal dimension character and other important surface roughness parameters via atomic force microscopy (AFM). Vertical aggregation of the successive layer was clearly verified in all quantitative approaches. Exceedingly, a better understanding in the direction of aggregation along with the growth mechanism was obtained by PSD analysis and the fractal dimension values gained around 2.27 for modified chitosan surface and 2.02 for GST immobilized chitosan modified screen-printed carbon basement could thus imply for the diffusion limited model in this growth mechanism.

show abstract

Atomic force microscopy in biomaterials surface science

Cited by 63 publications

References 123 publications

Fabrication of cell sheets with anisotropically aligned myotubes using thermally expandable micropatterned hydrogels

Fabrication of cell sheets with anisotropically aligned myotubes using thermally expandable micropatterned hydrogels

Atomic Force Microscopy: A Powerful Tool to Address Scaffold Design in Tissue Engineering

Nanostructural Characterization of Glutathione-S-Transferase Immobilizing Chitosan Modified Screen Printed Carbon Electrode by Atomic Force Microscopy

Contact Info

Product

Resources

About