2011
DOI: 10.1128/mmbr.00041-10
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Atomic Force Microscopy in Imaging of Viruses and Virus-Infected Cells

Abstract: SUMMARY Atomic force microscopy (AFM) can visualize almost everything pertinent to structural virology and at resolutions that approach those for electron microscopy (EM). Membranes have been identified, RNA and DNA have been visualized, and large protein assemblies have been resolved into component substructures. Capsids of icosahedral viruses and the icosahedral capsids of enveloped viruses have been seen at high resolution, in some cases sufficiently high to deduce the arrangement of … Show more

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Cited by 126 publications
(113 citation statements)
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“…2 b) presents a height of~90 nm and a diameter of 250 nm. The higher diameter compared with TEM data can be explained due to the tip-convolution effect (16). When the HIV-1 Gag VLP diameter is calculated in the mean height as performed by Faivre-Moskalenko et al (15), a diameter of~150 nm is obtained, which is in agreement with previous measurements performed with TEM.…”
Section: Identification Of Hiv-1 Gag Vlp By Afmsupporting
confidence: 84%
See 1 more Smart Citation
“…2 b) presents a height of~90 nm and a diameter of 250 nm. The higher diameter compared with TEM data can be explained due to the tip-convolution effect (16). When the HIV-1 Gag VLP diameter is calculated in the mean height as performed by Faivre-Moskalenko et al (15), a diameter of~150 nm is obtained, which is in agreement with previous measurements performed with TEM.…”
Section: Identification Of Hiv-1 Gag Vlp By Afmsupporting
confidence: 84%
“…AFM has already been used in virus and VLP characterization providing, to our knowledge, novel information about biological samples, such as morphology, structural data, and composition in ambient conditions (14)(15)(16). Interestingly, recent advances in multifrequency (MF) AFM modes offer a broad assessment of nanomechanical features of soft samples.…”
Section: Introductionmentioning
confidence: 99%
“…The bulbs, based on AFM height measurements, have a diameter when dry of about 25 nm, but drying produces at least a 25% shrinkage [1,2,4], so the bulbs are probably closer to 30 -35 nm in diameter. Assuming the globular masses to be composed of protein, this would suggest a total molecular weight of about 5 × 10 6 D, or about the same as a T = 3 icosahedral plant virus.…”
Section: Resultsmentioning
confidence: 99%
“…The substrate was freshly cleaved mica or mica coated with poly-l-lysine. Most details of the AFM methods have been presented in earlier papers [1][2][3][4]. Media samples of 1.5 Âľl to 5 Âľl containing virus were applied to the substrate and the virus allowed to sediment for 15 min to 30 min.…”
Section: Methodsmentioning
confidence: 99%
“…Spatially heterogeneous concentration profiles that form spontaneously in chemical reaction-diffusion systems represent the epitomical example of non-linear pattern formation, with immediate analogies to many other nonlinear systems [1][2][3][4][5][6][7][8][9][10][11]. While Turing predicted their spontaneous formation by a diffusion-driven instability in driven two-component systems in the 1950s [12], it took four decades to realize these experimentally in a chemical system [2,13,14].…”
Section: Introductionmentioning
confidence: 99%