Atomic force microscopy probing platelet activation behavior on titanium nitride nanocoatings for biomedical applications

Karagkiozaki, V.; Logothetidis, S.; Kalfagiannis, N.; Lousinian, S.; Giannoglou, Georgios

doi:10.1016/j.nano.2008.07.005

Cited by 51 publications

(34 citation statements)

References 27 publications

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“…During activation, a cascade of events is triggered leading to the structural alteration of the cells: spherical shape, extension of finger-like projections from the cell periphery, flattening of the cells onto the surface, extension of broad lamellae and formation of pseudonucleus (with a fried-egg appearance) due to the accumulation of the dense and alpha granules into the centre, and elaboration of unique blunt filopodia from cellular periphery based on new cytoplasmic actin filaments. 18 It should be mentioned that the long, thin filopodia contain bundles of F-actin and the flat lamellae contained an orthogonal array of short filaments. 18 Simultaneously, the activated platelets release a few chemical compounds from their granules that can induce further activation of other platelets.…”

Section: Ss and Cnt/dlc Thin Filmsmentioning

confidence: 99%

“…18 It should be mentioned that the long, thin filopodia contain bundles of F-actin and the flat lamellae contained an orthogonal array of short filaments. 18 Simultaneously, the activated platelets release a few chemical compounds from their granules that can induce further activation of other platelets. The next step is the platelet aggregation, an essential procedure for thrombus formation.…”

Section: Ss and Cnt/dlc Thin Filmsmentioning

confidence: 99%

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Novel nanostructured biomaterials: implications for coronary stent thrombosis

Karagkiozaki¹,

Karagiannidis²,

Kalfagiannis³

et al. 2012

IJN

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Background: Nanomedicine has the potential to revolutionize medicine and help clinicians to treat cardiovascular disease through the improvement of stents. Advanced nanomaterials and tools for monitoring cell-material interactions will aid in inhibiting stent thrombosis. Although titanium boron nitride (TiBN), titanium diboride, and carbon nanotube (CNT) thin films are emerging materials in the biomaterial field, the effect of their surface properties on platelet adhesion is relatively unexplored. Objective and methods: In this study, novel nanomaterials made of amorphous carbon, CNTs, titanium diboride, and TiBN were grown by vacuum deposition techniques to assess their role as potential stent coatings. Platelet response towards the nanostructured surfaces of the samples was analyzed in line with their physicochemical properties. As the stent skeleton is formed mainly of stainless steel, this material was used as reference material. Platelet adhesion studies were carried out by atomic force microscopy and scanning electron microscopy observations. A cell viability study was performed to assess the cytocompatibility of all thin film groups for 24 hours with a standard immortalized cell line. Results: The nanotopographic features of material surface, stoichiometry, and wetting properties were found to be significant factors in dictating platelet behavior and cell viability. The TiBN films with higher nitrogen contents were less thrombogenic compared with the biased carbon films and control. The carbon hybridization in carbon films and hydrophilicity, which were strongly dependent on the deposition process and its parameters, affected the thrombogenicity potential. The hydrophobic CNT materials with high nanoroughness exhibited less hemocompatibility in comparison with the other classes of materials. All the thin film groups exhibited good cytocompatibility, with the surface roughness and surface free energy influencing the viability of cells.

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Section: Ss and Cnt/dlc Thin Filmsmentioning

confidence: 99%

Section: Ss and Cnt/dlc Thin Filmsmentioning

confidence: 99%

Novel nanostructured biomaterials: implications for coronary stent thrombosis

Karagkiozaki¹,

Karagiannidis²,

Kalfagiannis³

et al. 2012

IJN

Self Cite

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“…17 These structural alterations of platelets during activation can be imaged by AFM in detail, as described in previous studies. 18 The cells then release biochemical compounds (eg, serotonin, thromboglobulin, platelet factor IV, thromboxane) for further activation and aggregation. 17 In Figure 6A, the platelets after 1 hour of adhesion onto the drug-free sample were found at different stages of activation: (a) egg-like type platelets, less activated, denoted by circles; (b) more activated platelets with pseudonucleus and pseudopodia, as presented by the blue squares; and (c) platelet aggregation (height ca.…”

Section: Platelet Adhesion Studies Onto Drug-free and Drug-loaded Trimentioning

confidence: 99%

Development of a nanoporous and multilayer drug-delivery platform for medical implants

Karagkiozaki

Vavoulidis²,

Karagiannidis³

et al. 2012

IJN

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Biodegradable polymers can be applied to a variety of implants for controlled and local drug delivery. The aim of this study is to develop a biodegradable and nanoporous polymeric platform for a wide spectrum of drug-eluting implants with special focus on stent-coating applications. It was synthesized by poly(DL-lactide-co-glycolide) (PLGA 65:35, PLGA 75:25) and polycaprolactone (PCL) in a multilayer configuration by means of a spin-coating technique. The antiplatelet drug dipyridamole was loaded into the surface nanopores of the platform. Surface characterization was made by atomic force microscopy (AFM) and spectroscopic ellipsometry (SE). Platelet adhesion and drug-release kinetic studies were then carried out. The study revealed that the multilayer films are highly nanoporous, whereas the single layers of PLGA are atomically smooth and spherulites are formed in PCL. Their nanoporosity (pore diameter, depth, density, surface roughness) can be tailored by tuning the growth parameters (eg, spinning speed, polymer concentration), essential for drug-delivery performance. The origin of pore formation may be attributed to the phase separation of polymer blends via the spinodal decomposition mechanism. SE studies revealed the structural characteristics, film thickness, and optical properties even of the single layers in the triple-layer construct, providing substantial information for drug loading and complement AFM findings. Platelet adhesion studies showed that the dipyridamole-loaded coatings inhibit platelet aggregation that is a prerequisite for clotting. Finally, the films exhibited sustained release profiles of dipyridamole over 70 days. These results indicate that the current multilayer phase therapeutic approach constitutes an effective drug-delivery platform for drugeluting implants and especially for cardiovascular stent applications.

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“…These features affect such functional properties as hardness, wear resistance, mechanical properties, biological properties and wettability. In order to modify the properties of biomaterials, different methods of surface treatment are used, ranging from electrochemical oxidation, thermal spraying, ion implantation, laser treatment (PLD), chemical vapour deposition (RFCVD, MWCVD), to hybrid and glow-discharge methods [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20]. Selection of the appropriate method depends on the final application of the material, the implant's shape and the expected characteristics of the produced surface layers.…”

Section: Introductionmentioning

confidence: 99%

The Importance Of Surface Topography For The Biological Properties Of Nitrided Diffusion Layers Produced On Ti6Al4V Titanium Alloy

Wierzchoń¹,

Czarnowska²,

Morgiel³

et al. 2015

Archives of Metallurgy and Materials

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Diffusion nitrided layers produced on titanium and its alloys are widely studied in terms of their application for cardiac and bone implants. The influence of the structure, the phase composition, topography and surface morphology on their biological properties is being investigated. The article presents the results of a study of the topography (nanotopography) of the surface of TiN+Ti 2 N+αTi(N) nitrided layers produced in low-temperature plasma on Ti6Al4V titanium alloy and their influence on the adhesion of blood platelets and their aggregates. The TEM microstructure of the produced layers have been examined and it was demonstrated that the interaction between platelets and the surface of the titanium implants subjected to glow-discharge nitriding can be shaped via modification of the roughness parameters of the external layer of the TiN titanium nitride nanocrystalline zone.Keywords: Ti6Al4V titanium alloy, glow discharge assisted nitriding, surface topography, wettability, blood platelets Dyfuzyjne warstwy azotowane na tytanie i jego stopach są szeroko badane m. in. w aspekcie zastosowań na implanty kardiologiczne i kostne. Stąd też analizowany jest wpływ struktury składu fazowego, topografii i morfologii powierzchni na ich właściwości biologiczne. W artykule przedstawiono wyniki badań wpływu topografii (nanotopografii) powierzchni warstw azotowanych -TiN+Ti 2 N+αTi(N) wytwarzanych w niskotemperaturowej plazmie na stopie tytanu Ti6Al4V na adhezję płytek krwi i ich aglomeratów. Omówiono mikrostrukturę (TEM) wytwarzanych warstw i wykazano, że poprzez stan chropowatości powierzchni zewnętrznej strefy warstwy azotowanej -nanokrystalicznego azotku tytanu (TiN) można kształtować oddziaływanie płytek krwi z powierzchnią implantów tytanowych poddanych procesowi azotowania jarzeniowego.

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Atomic force microscopy probing platelet activation behavior on titanium nitride nanocoatings for biomedical applications

Cited by 51 publications

References 27 publications

Novel nanostructured biomaterials: implications for coronary stent thrombosis

Novel nanostructured biomaterials: implications for coronary stent thrombosis

Development of a nanoporous and multilayer drug-delivery platform for medical implants

The Importance Of Surface Topography For The Biological Properties Of Nitrided Diffusion Layers Produced On Ti6Al4V Titanium Alloy

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