Atomic structure of the regulatory TGS domain of Rel protein from <i>Mycobacterium tuberculosis</i> and its interaction with deacylated tRNA

Shin, Joon; Singal, Bharti; Grüber, Ardina; Wong, David Meng Kit; Ragunathan, Priya; Grüber, Gerhard

doi:10.1002/1873-3468.14236

Cited by 4 publications

(2 citation statements)

References 44 publications

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“…AtCRSH does not possess a functional hydrolase domain and in AtRSH2/3, both SYNTH and HD domains are active [356]. In addition to HD and SYNTH domains, RSH possess chloroplast transit peptide [357], TGS (RSH1, RSH2/3), and ACT domains (RSH1) which were proposed to act as regulatory-or ligand-binding domains [358]. CRSH are the only proteins involved in alarmones metabolism that possess two EF hand motifs and are activated by Ca 2+ ions [359].…”

Section: The Stringent Response In Plant-microorganism Interactionsmentioning

confidence: 99%

The Importance of Microorganisms for Sustainable Agriculture—A Review

2022

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In the face of climate change, progressive degradation of the environment, including agricultural land negatively affecting plant growth and development, endangers plant productivity. Seeking efficient and sustainable agricultural techniques to replace agricultural chemicals is one of the most important challenges nowadays. The use of plant growth-promoting microorganisms is among the most promising approaches; however, molecular mechanisms underneath plant–microbe interactions are still poorly understood. In this review, we summarized the knowledge on plant–microbe interactions, highlighting the role of microbial and plant proteins and metabolites in the formation of symbiotic relationships. This review covers rhizosphere and phyllosphere microbiomes, the role of root exudates in plant–microorganism interactions, the functioning of the plant’s immune system during the plant–microorganism interactions. We also emphasized the possible role of the stringent response and the evolutionarily conserved mechanism during the established interaction between plants and microorganisms. As a case study, we discussed fungi belonging to the genus Trichoderma. Our review aims to summarize the existing knowledge about plant–microorganism interactions and to highlight molecular pathways that need further investigation.

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Section: The Stringent Response In Plant-microorganism Interactionsmentioning

confidence: 99%

The Importance of Microorganisms for Sustainable Agriculture—A Review

2022

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“…RSH2 and RSH3 in B. napus possess functional SYNTH and HD domains [56]. Other conserved domains present in plant RSHs are the EF-hand motif in CRSH that is responsible for binding Ca 2+ ions [57] and the ACT and TGS domains allowing for the recognition and binding of ligands [58]. At the N-terminus of plant RSH proteins, there is also a chloroplast-targeting sequence [55].…”

Section: Introductionmentioning

confidence: 99%

Trichoderma viride Colonizes the Roots of Brassica napus L., Alters the Expression of Stress-Responsive Genes, and Increases the Yield of Canola under Field Conditions during Drought

Garstecka,

Antoszewski,

Mierek-Adamska

et al. 2023

IJMS

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In this work, we present the results of the inoculation of canola seeds (Brassica napus L.) with Trichoderma viride strains that promote the growth of plants. Seven morphologically different strains of T. viride (TvI-VII) were shown to be capable of synthesizing auxins and exhibited cellulolytic and pectinolytic activities. To gain a deeper insight into the molecular mechanisms underlying canola–T. viride interactions, we analyzed the canola stress genes metallothioneins (BnMT1-3) and stringent response genes (BnRSH1-3 and BnCRSH). We demonstrated the presence of cis-regulatory elements responsive to fungal elicitors in the promoter regions of B. napus MT and RSH genes and observed changes in the levels of the transcripts of the above-mentioned genes in response to root colonization by the tested fungal strains. Of the seven tested strains, under laboratory conditions, T. viride VII stimulated the formation of roots and the growth of canola seedlings to the greatest extent. An experiment conducted under field conditions during drought showed that the inoculation of canola seeds with a suspension of T. viride VII spores increased yield by 16.7%. There was also a positive effect of the fungus on the height and branching of the plants, the number of siliques, and the mass of a thousand seeds. We suggest that the T. viride strain TvVII can be used in modern sustainable agriculture as a bioinoculant and seed coating to protect B. napus from drought.

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Mycobacterial Regulatory Systems Involved in the Regulation of Gene Expression Under Respiration-Inhibitory Conditions

Lee

et al. 2023

J Microbiol.

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Atomic structure of the regulatory TGS domain of Rel protein from Mycobacterium tuberculosis and its interaction with deacylated tRNA

Cited by 4 publications

References 44 publications

The Importance of Microorganisms for Sustainable Agriculture—A Review

The Importance of Microorganisms for Sustainable Agriculture—A Review

Trichoderma viride Colonizes the Roots of Brassica napus L., Alters the Expression of Stress-Responsive Genes, and Increases the Yield of Canola under Field Conditions during Drought

Mycobacterial Regulatory Systems Involved in the Regulation of Gene Expression Under Respiration-Inhibitory Conditions

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