ATP/ADP antiporter is involved in uncoupling of plant mitochondria induced by low concentrations of palmitate

Abstract

Carboxyatractyloside partially restored the transmembrane electrical potential difference (d'p) dissipated by low concentrations of pahnitate in pea stem mitochondria. This effect was more marked when mitochondria from sunflower were assayed. It is sugeested that the A'WADP translocator is involved in the free fatty acid-induced uncoupling of oxidative phosphorylation in plant mitocdondria, o&when its level is sufficiently high and the concentration of the fatty acid is low to collapse only partially AY.

Fatty acids induced uncoupling of Saccharomyces cerevisiae mitochondria requires an intact ADP/ATP carrier

“…Similar effect of ADP/ATP carrier inhibitors on the fatty acids induced uncoupling of mammalian [5,6] and plant [14] mitochondria has been interpreted to indicate that the carrier is involved in the fatty acids mediated H + permeability of inner mitochondrial membrane. The sensitivity of fatty acids-induced uncoupling to the different ADP/ATP transport inhibitors markedly varies with the nature of the inhibitor and with the source of mitochondria used [5,6,14,15]. In addition, it might also reflect an indirect action of these compounds on A\|/.…”

Plant mitochondrial carriers: an overview

“…Similar effects of fatty acids have also been described in plants. The addition of a low palmitate concentration to pea or sunflower mitochondria decreases the Zc, and CATR partially restores it [75], suggesting that also in plants ANT could be involved in uncoupling by fatty acids. Finally, when grown at 14°C, seedlings of maize lines selected for high germination at 9.5°C (C4-H) showed a higher activity of reconstituted ANT than seedlings selected for low germination (C4-L).…”

Effect of 6‐ketocholestanol on FCCP‐ and DNP‐induced uncoupling in plant mitochondria

“…Oxygen consumption was recorded as described in [6]. To estimate the A7 ~ changes, safranin O was used [8][9][10].…”

“…Pea stem and sunflower hypocotyl mitochondria were found to be similar with mammalian mitochondria in the participation of ATP/ADP antiporter in free fatty acid-induced uncoupling [6,7]. Therefore, it was interesting to study if the FCCP-induced *Corresponding author.…”