ATP-Binding Cassette G Transporters and Their Multiple Roles Especially for Male Fertility in Arabidopsis, Rice and Maize

Shu, Wu; Fang, Chaowei; Li, Ziwen; Li, Han; Pan, Shuangshuang; Wu, Yuru; An, Xianghai; Long, Yan; Wan, Xiangyuan

doi:10.3390/ijms23169304

Cited by 13 publications

(7 citation statements)

References 75 publications

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“…There were 12 TASs of the WW condition located on the 35,474,299–35,698,370 bp interval in chromosome C04 ( Additional File 7 ; Figures 4A–D ). This genomic region contained four genes of ABC transporter G superfamily ( ABCG16 , ABCG17 , ABCG18 , and ABCG19 ) which contribute to cytokinin transport in the shoot and enhance the tiller number, grain number per panicle, and grain yield ( Wu et al., 2022a ). Four SNPs, Chr14:35642269 (A/G), Chr14:35642321 (A/C), Chr14:35642324 (A/C), and Chr14:35643023 (A/G), in an LD block, were located within the ABCG16 sequence ( Figures 4C, E, F ).…”

Section: Resultsmentioning

confidence: 99%

Dissection of quantitative trait nucleotides and candidate genes associated with agronomic and yield-related traits under drought stress in rapeseed varieties: integration of genome-wide association study and transcriptomic analysis

Salami,

Heidari,

Alizadeh

et al. 2024

Front. Plant Sci.

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IntroductionAn important strategy to combat yield loss challenge is the development of varieties with increased tolerance to drought to maintain production. Improvement of crop yield under drought stress is critical to global food security.MethodsIn this study, we performed multiomics analysis in a collection of 119 diverse rapeseed (Brassica napus L.) varieties to dissect the genetic control of agronomic traits in two watering regimes [well-watered (WW) and drought stress (DS)] for 3 years. In the DS treatment, irrigation continued till the 50% pod development stage, whereas in the WW condition, it was performed throughout the whole growing season.ResultsThe results of the genome-wide association study (GWAS) using 52,157 single-nucleotide polymorphisms (SNPs) revealed 1,281 SNPs associated with traits. Six stable SNPs showed sequence variation for flowering time between the two irrigation conditions across years. Three novel SNPs on chromosome C04 for plant weight were located within drought tolerance-related gene ABCG16, and their pleiotropically effects on seed weight per plant and seed yield were characterized. We identified the C02 peak as a novel signal for flowering time, harboring 52.77% of the associated SNPs. The 288-kbps LD decay distance analysis revealed 2,232 candidate genes (CGs) associated with traits. The CGs BIG1-D, CAND1, DRG3, PUP10, and PUP21 were involved in phytohormone signaling and pollen development with significant effects on seed number, seed weight, and grain yield in drought conditions. By integrating GWAS and RNA-seq, 215 promising CGs were associated with developmental process, reproductive processes, cell wall organization, and response to stress. GWAS and differentially expressed genes (DEGs) of leaf and seed in the yield contrasting accessions identified BIG1-D, CAND1, and DRG3 genes for yield variation.DiscussionThe results of our study provide insights into the genetic control of drought tolerance and the improvement of marker-assisted selection (MAS) for breeding high-yield and drought-tolerant varieties.

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Section: Resultsmentioning

confidence: 99%

Dissection of quantitative trait nucleotides and candidate genes associated with agronomic and yield-related traits under drought stress in rapeseed varieties: integration of genome-wide association study and transcriptomic analysis

Salami,

Heidari,

Alizadeh

et al. 2024

Front. Plant Sci.

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“…The precursors of sporopollenin, cutin, and wax are synthesized in anther tapetum, and catalyzed by a series of lipid metabolism-related GMS gene-encoding enzymes, such as ZmMs25/ZmFAR1 encoding a plastid-localized fatty acyl reductase [ 48 , 49 ], ZmMs33 encoding a glycerol-3-phosphate acyltransferase [ 50 , 51 , 52 , 53 ], ZmMs30 encoding a GDSL lipase [ 54 ], and ZmPKSB encoding a polyketide synthase [ 55 ]. Then, the lipid precursors are transported into the pollen surface and anther outer wall by ABCGs such as ZmABCG26 and ZmMs13/ZmABCG2a [ 48 , 56 , 57 ], LTPs such as Zmms44 [ 32 ], and other transporters [ 47 ]. Loss functions of the anther-specific or high-expressed LTP genes often lead to defective anther cuticle and pollen wall formation and thus male sterility in plants [ 58 ].…”

Section: Resultsmentioning

confidence: 99%

A Systematic Investigation of Lipid Transfer Proteins Involved in Male Fertility and Other Biological Processes in Maize

Fang

Shu

et al. 2023

IJMS

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Plant lipid transfer proteins (LTPs) play essential roles in various biological processes, including anther and pollen development, vegetative organ development, seed development and germination, and stress response, but the research progress varies greatly among Arabidopsis, rice and maize. Here, we presented a preliminary introduction and characterization of the whole 65 LTP genes in maize, and performed a phylogenetic tree and gene ontology analysis of the LTP family members in maize. We compared the research progresses of the reported LTP genes involved in male fertility and other biological processes in Arabidopsis and rice, and thus provided some implications for their maize orthologs, which will provide useful clues for the investigation of LTP transporters in maize. We predicted the functions of LTP genes based on bioinformatic analyses of their spatiotemporal expression patterns by using RNA-seq and qRT-PCR assays. Finally, we discussed the advances and challenges in substrate identification of plant LTPs, and presented the future research directions of LTPs in plants. This study provides a basic framework for functional research and the potential application of LTPs in multiple plants, especially for male sterility research and application in maize.

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“…Of maize lipid metabolic GMS genes, nine are involved in lipid biosynthesis, such as ZmMs20/IRREGULAR POLLEN EXINE1 ( ZmIPE1 ) (Chen et al ., 2017 ; Wang et al ., 2019 ), ZmMs25/ZmMs6021 (Tian et al ., 2017 ; Zhang et al ., 2021 ), ZmMs26 (Djukanovic et al ., 2013 ; Singh et al ., 2017 ), ZmMs30 (An et al ., 2019 ), ZmMs33 (Li et al ., 2022 , 2023 ; Xie et al ., 2018 ; Zhang et al ., 2018b ; Zhu et al ., 2019 , 2020 ), ZmMs45 (Cigan et al ., 2001 ), ABNORMAL POLLEN VACUOLATION1 ( ZmAPV1 ) (Somaratne et al ., 2017 ), ZmIPE2 (Huo et al ., 2020 ) and POLYKETIDE SYNTHASE B ( ZmPKSB ) (Liu et al ., 2022 ). The rest encode lipid transfer proteins or ABCG transporters that are involved in lipid transport in maize anther (Fang et al ., 2023 ; Wu et al ., 2022 ), including ZmMs2/ZmABCG26 (Choi et al ., 2011 ; Jiang et al ., 2021b ; Xu et al ., 2021 ), ZmMs13/ZmABCG2a (Fang et al ., 2022 ) and ZmMs44 (Fox et al ., 2017 ). All mutants of these genes exhibit defective pollen exine and anther cuticle phenotypes.…”

Section: Introductionmentioning

confidence: 99%

CRISPR/Cas9‐based genome editing of 14 lipid metabolic genes reveals a sporopollenin metabolon ZmPKSB‐ZmTKPR1‐1/‐2 required for pollen exine formation in maize

An,

Zhang,

Jiang

et al. 2023

Plant Biotechnology Journal

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SummaryLipid biosynthesis and transport are essential for plant male reproduction. Compared with Arabidopsis and rice, relatively fewer maize lipid metabolic genic male‐sterility (GMS) genes have been identified, and the sporopollenin metabolon in maize anther remains unknown. Here, we identified two maize GMS genes, ZmTKPR1‐1 and ZmTKPR1‐2, by CRISPR/Cas9 mutagenesis of 14 lipid metabolic genes with anther stage‐specific expression patterns. Among them, tkpr1‐1/‐2 double mutants displayed complete male sterility with delayed tapetum degradation and abortive pollen. ZmTKPR1‐1 and ZmTKPR1‐2 encode tetraketide α‐pyrone reductases and have catalytic activities in reducing tetraketide α‐pyrone produced by ZmPKSB (polyketide synthase B). Several conserved catalytic sites (S128/130, Y164/166 and K168/170 in ZmTKPR1‐1/‐2) are essential for their enzymatic activities. Both ZmTKPR1‐1 and ZmTKPR1‐2 are directly activated by ZmMYB84, and their encoded proteins are localized in both the endoplasmic reticulum and nuclei. Based on protein structure prediction, molecular docking, site‐directed mutagenesis and biochemical assays, the sporopollenin biosynthetic metabolon ZmPKSB‐ZmTKPR1‐1/‐2 was identified to control pollen exine formation in maize anther. Although ZmTKPR1‐1/‐2 and ZmPKSB formed a protein complex, their mutants showed different, even opposite, defective phenotypes of anther cuticle and pollen exine. Our findings discover new maize GMS genes that can contribute to male‐sterility line‐assisted maize breeding and also provide new insights into the metabolon‐regulated sporopollenin biosynthesis in maize anther.

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ATP-Binding Cassette G Transporters and Their Multiple Roles Especially for Male Fertility in Arabidopsis, Rice and Maize

Cited by 13 publications

References 75 publications

Dissection of quantitative trait nucleotides and candidate genes associated with agronomic and yield-related traits under drought stress in rapeseed varieties: integration of genome-wide association study and transcriptomic analysis

Dissection of quantitative trait nucleotides and candidate genes associated with agronomic and yield-related traits under drought stress in rapeseed varieties: integration of genome-wide association study and transcriptomic analysis

A Systematic Investigation of Lipid Transfer Proteins Involved in Male Fertility and Other Biological Processes in Maize

CRISPR/Cas9‐based genome editing of 14 lipid metabolic genes reveals a sporopollenin metabolon ZmPKSB‐ZmTKPR1‐1/‐2 required for pollen exine formation in maize

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