ATP-Binding Cassette Transporters: Snap-on Complexes?

Younus, Iqra; Kochkina, Sofia; Choi, Cheri C.; Sun, Wenping; Ford, Robert C.

doi:10.1007/978-3-031-00793-4_2

Cited by 1 publication

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“…znuABC is a high-affinity Zn 2+ transporter composed of the soluble periplasmic binding protein ZnuA (Formerly YcdH), the high-permeability transmembrane protein ZnuB (Formerly YceA) and the ATPase ZnuC (Formerly YcdI), and is regulated by Zur (zinc uptake system) ( 23 ). znuABC belongs to the ABC transporter, which is one of the largest transporter gene families that transport a variety of substrates across the membrane by utilizing ATP energy ( 24 , 25 ). These transporters have two transmembrane domains (TMDs) and two nucleotide binding domains (NBDs).…”

Section: Introductionmentioning

confidence: 99%

Virulence regulation of Zn2+ uptake system znuABC on mesophilic Aeromonas salmonicida SRW-OG1

et al. 2023

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Psychrophilic Aeromonas salmonicida could not grow above 25°C and therefore thought unable to infect mammals and humans. In our previous study, a mesophilic A. salmonicida SRW-OG1 was isolated from Epinephelus coioides with furunculosis. Through the analysis of preliminary RNA-seq, it was found that the Zn2+ uptake related genes znuA, znuB and znuC might be involved in the virulence regulation of A. salmonicida SRW-OG1. Therefore, the purpose of this study was to explore the effect of znuABC silencing on the virulence regulation of A. salmonicida SRW-OG1. The results showed that the growth of the znuA-RNAi, znuB-RNAi, and znuC-RNAi strains was severely restricted under the Fe2+ starvation, but surprisingly there was no significant difference under the Zn2+ restriction. In the absence of Zn2+ and Fe2+, the expression level of znuABC was significantly increased. The motility, biofilm formation, adhesion and hemolysis of the znuA-RNAi, znuB-RNAi, and znuC-RNAi strains were significantly reduced. We also detected the expression of znuABC under different growth periods, temperatures, pH, as well as Cu2+ and Pb2+ stresses. The results showed that znuABC was significantly up-regulated in the logarithmic phase and the decline phase of A. salmonicida. Interestingly, the trend of expression levels of the znuABC at 18, 28, and 37°C was reversed to another Zn2+ uptake related gene zupT. Taken together, these indicated that the znuABC was necessary for A. salmonicida SRW-OG1 pathogenicity and environmental adaptability, and was cross regulated by iron starvation, but it was not irreplaceable for A. salmonicida SRW-OG1 Zn2+ uptake in the host.

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Section: Introductionmentioning

confidence: 99%