ATP-Dependent Silver Transport across the Basolateral Membrane of Rainbow Trout Gills

Bury, Nicolas R.; Grosell, Martin; Grover, Ashok K.; Wood, Chris M.

doi:10.1006/taap.1999.8706

Cited by 96 publications

(71 citation statements)

References 46 publications

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“…In contrast, Ag in nanoparticle form can constantly dissolve and travel to the other places. Additionally, it is rather difficult for Ag ions to cross cellular membrane and the biological barriers, 40,41 and our recent study also demonstrated that the bioavailability of Ag ions is minimal to mammalian cells in mice. 45 Nonetheless, a mild embryonic toxicity was observed in mice challenged by nAg or Ag ions both at 108 μg/kg, as the numbers of viable babies in these two groups were reduced compared to the control, despite the absence of statistical significance ( Figure 9H, P > 0.05).…”

Section: Articlementioning

confidence: 88%

Silver Nanoparticles Induced RNA Polymerase-Silver Binding and RNA Transcription Inhibition in Erythroid Progenitor Cells

et al. 2013

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Due to its antimicrobial activity, nanosilver (nAg) has become the most widely used nanomaterial. Thus far, the mechanisms responsible for nAg-induced antimicrobial properties and nAg-mediated toxicity to organisms have not been clearly recognized. Silver (Ag) ions certainly play a crucial role, and the form of nanoparticles can change the dissolution rate, bioavailability, biodistribution, and cellular uptake of Ag. However, whether nAg exerts direct "particle-specific" effects has been under debate. Here we demonstrated that nAg exhibited a robust inhibition on RNA polymerase activity and overall RNA transcription through direct Ag binding to RNA polymerase, which is separated from the cytotoxicity pathway induced by Ag ions. nAg treatment in vitro resulted in reduced hemoglobin concentration in erythroid cells; in vivo administration of nAg in mice caused profound reduction of hemoglobin content in embryonic erythrocytes, associated with anemia in the embryos.Embryonic anemia and general proliferation deficit due to the significant inhibition on RNA synthesis, at least partially, accounted for embryonic developmental retardation upon nAg administration. To date, there is no conclusive answer to the sources of nAg-mediated toxicity: Ag ions or "particlespecific" effects, or both. We here demonstrated that both Ag ions and nAg particles simultaneously existed inside cells, demonstrating the "Trojan horse" effects of nAg particles in posing biological impacts on erythroid cells. Moreover, our results suggested that "particle-specific" effects could be the predominant mediator in eliciting biological influences on erythroid cells under relatively low concentrations of nAg exposure. The combined data highlighted the inhibitory effect of nAg on RNA polymerase activity through a direct reciprocal interaction.

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Section: Articlementioning

confidence: 88%

Silver Nanoparticles Induced RNA Polymerase-Silver Binding and RNA Transcription Inhibition in Erythroid Progenitor Cells

et al. 2013

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“…Inhibition of Cu uptake by Ag has been shown in bacteria [105], plant [106], fish [107] and humans [103,108]. Moreover, recently, it has been demonstrated that CTR1 is an important Ag transporter.…”

Section: Molecular Mechanisms Of Ag Uptakementioning

confidence: 99%

“…ATPase [107]. This uptake route for Cu and Ag is likely to occur only in the freshwater fish, where external Na þ is only a few millimolar or less [117].…”

Section: Molecular Mechanisms Of Ag Uptakementioning

confidence: 99%

Bioavailability of silver nanoparticles and ions: from a chemical and biochemical perspective

Behra

Sigg

Clift

et al. 2013

J. R. Soc. Interface.

289

184

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Owing to their antimicrobial properties, silver nanoparticles (NPs) are the most commonly used engineered nanomaterial for use in a wide array of consumer and medical applications. Many discussions are currently ongoing as to whether or not exposure of silver NPs to the ecosystem (i.e. plants and animals) may be conceived as harmful or not. Metallic silver, if released into the environment, can undergo chemical and biochemical conversion which strongly influence its availability towards any biological system. During this process, in the presence of moisture, silver can be oxidized resulting in the release of silver ions. To date, it is still debatable as to whether any biological impact of nanosized silver is relative to either its size, or to its ionic constitution. The aim of this review therefore is to provide a comprehensive, interdisciplinary overview-for biologists, chemists, toxicologists as well as physicists-regarding the production of silver NPs, its (as well as in their ionic form) chemical and biochemical behaviours towards/within a multitude of relative and realistic biological environments and also how such interactions may be correlated across a plethora of different biological organisms.

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“…Uptake and toxicity of Ag + ions is mainly via competition with Na + and ATP-dependent transport and disruption of membrane sodium channels, as well as the depletion of the cellular energy system (Bianchini and Wood, 2003;Bury and Wood, 1999;Bury et al, 1999;Galvez and Wood, 2002;Hidalgo and Dominguez, 1998;Pedroso et al, 2007). In contrast, Ag NPs are taken up by cells through diffusion (Lee et al, 2007a(Lee et al, , 2007b, pinocytosis (Luoma, 2008) and phagocytosis , as well as via disturbance of membrane components.…”

Section: Ag Toxicitymentioning

confidence: 99%

Toxic effects and bioaccumulation of nano-, micron- and ionic-Ag in the polychaete, Nereis diversicolor

Banta

Selck

et al. 2011

Aquatic Toxicology

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ATP-Dependent Silver Transport across the Basolateral Membrane of Rainbow Trout Gills

Cited by 96 publications

References 46 publications

Silver Nanoparticles Induced RNA Polymerase-Silver Binding and RNA Transcription Inhibition in Erythroid Progenitor Cells

Silver Nanoparticles Induced RNA Polymerase-Silver Binding and RNA Transcription Inhibition in Erythroid Progenitor Cells

Bioavailability of silver nanoparticles and ions: from a chemical and biochemical perspective

Toxic effects and bioaccumulation of nano-, micron- and ionic-Ag in the polychaete, Nereis diversicolor

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