ATP depletion inhibits retraction of chromatophores induced by GABA in cephalopod Todarodes pacificus

Kinoshita, Yasunori; Kato, Sanae; Yoshioka, Toshikazu; Konno, Kunihiko

doi:10.1007/s12562-013-0630-9

Cited by 3 publications

(4 citation statements)

References 18 publications

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“…The supernatant was filtered through a 0.45 µm filter (Merck Millipore, Burlington, MA, USA), and IMP was measured by HPLC using a column (TSK gel ODS 80Ts, 4.6 × 250 mm; Tosoh, Tokyo, Japan). Chromatographic separation was obtained by increasing the acetonitrile concentration in the mobile phase of 0.1 M NaH 2 PO 4 (pH 4.1) 46 .…”

Section: Identification and Cloning Of Nt5ea And Nt5eb In Medakamentioning

confidence: 99%

“…The supernatant was filtered through a 0.45 µm filter (Merck Millipore), and components were separated by HPLC using a column (TSK gel ODS 80Ts, 4.6 × 250 mm; Tosoh) and evaluated. Separation was achieved by increasing the acetonitrile concentration in 0.1 M NaH 2 PO 4 (pH 4.1) 46 . The K-value was calculated as the percentage of HxR and Hx to the sum of ATP and degradation products, as follows 48 :…”

Section: Quantification Of Atp-related Compounds In Musclementioning

confidence: 99%

See 1 more Smart Citation

Targeted deletion of ecto-5′-nucleotidase results in retention of inosine monophosphate content in postmortem muscle of medaka (Oryzias latipes)

Murakami

Andô

Futamata

et al. 2022

Sci Rep

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Inosine monophosphate (IMP) is an important indicator of meat freshness and contributes to its umami taste. An attractive strategy for enhancing umami is to suppress the IMP-degrading activity and increase the IMP content in the skeletal muscle through genome editing technology using the CRISPR-Cas9 system. However, the molecular mechanisms underlying IMP degradation remain unclear. We cloned two ecto-5′-nucleotidase genes, designated as ecto-5′-nucleotidase-a (nt5ea) and ecto-5′-nucleotidase-b (nt5eb), from medaka (Oryzias latipes), a vertebrate model organism. Expression analysis using embryos showed that nt5ea or nt5eb overexpression remarkably upregulated IMP degradation, and that the IMP-degrading activity was higher in Nt5ea than in Nt5eb. Furthermore, we established frame-shifted or large deletion (lacking nt5ea or nt5eb locus) mutant strains and assayed the effects of gene disruptions on the amount of IMP in skeletal muscle. The nt5ea-deficient medaka showed considerable higher levels of IMP at 48 h postmortem than did the wild-type fish. The nt5eb mutants also exhibited higher IMP contents than that in the wild types, but the increase was less than that in the nt5ea mutants. Our results demonstrated that nt5e is an important regulator of IMP levels in skeletal muscle and that its loss of function was effective in maintaining IMP content.

show abstract

Section: Identification and Cloning Of Nt5ea And Nt5eb In Medakamentioning

confidence: 99%

Section: Quantification Of Atp-related Compounds In Musclementioning

confidence: 99%

Targeted deletion of ecto-5′-nucleotidase results in retention of inosine monophosphate content in postmortem muscle of medaka (Oryzias latipes)

Murakami

Andô

Futamata

et al. 2022

Sci Rep

View full text Add to dashboard Cite

show abstract

“…The supernatant was ltered through a 0.45 µm lter (Merck Millipore, Burlington, MA, USA), and IMP was measured by HPLC using a column (TSK gel ODS 80Ts, 4.6 × 250 mm; Tosoh, Tokyo, Japan). Chromatographic separation was obtained by increasing the acetonitrile concentration in the mobile phase 0.1 M NaH 2 PO 4 (pH 4.1) [47] . The measured amounts of IMP were normalized to the luminescence intensities of LUC to accurately compare the activities of Nt5ea and Nt5eb against IMP.…”

Section: Assay Of Imp-degrading Activity In Nt5ea and Nt5ebmentioning

confidence: 99%

“…The supernatant was ltered through a 0.45 µm lter (Merck Millipore), and components were separated by HPLC using a column (TSK gel ODS 80Ts, 4.6 × 250 mm; Tosoh) and evaluated. Separation was achieved by increasing the acetonitrile concentration in 0.1 M NaH 2 PO 4 (pH 4.1) [47] . The K-value was calculated as the percentage of HxR and Hx to the sum of ATP and degradation products, as follows [48] : K-value (%) = (HxR + Hx)/(ATP + ADP + AMP + IMP + HxR + Hx) × 100…”

Section: Establishment Of Knockout Strains With Large Deletionmentioning

confidence: 99%

Targeted deletion of ecto-5′-nucleotidase results in retention of inosine monophosphate content in postmortem muscle of medaka (Oryzias latipes)

Murakami

Andô

Futamata

et al. 2022

Preprint

View full text Add to dashboard Cite

Inosine monophosphate (IMP) is an important indicator of meat freshness and contributes to its umami taste. An attractive strategy for enhancing umami is to suppress the IMP-degrading activity and increase the IMP content in the skeletal muscle through genome editing technology using the CRISPR-Cas9 system. However, the molecular mechanisms underlying IMP degradation remain unclear. We cloned two ecto-5’-nucleotidase genes, designated as ecto-5′-nucleotidase-a (nt5ea) and ecto-5′-nucleotidase-b (nt5eb), from medaka (Oryzias latipes), a vertebrate model organism. Expression analysis using embryos showed that nt5ea or nt5eb overexpression remarkably upregulated IMP degradation, and that the IMP degrading activity was higher in Nt5ea than in Nt5eb. Furthermore, we established frame-shifted or large deletion (lacking nt5ea or nt5eb locus) mutant strains and assayed the effects of gene disruptions on the amount of IMP in skeletal muscle. The nt5ea-deficient medaka showed significantly higher levels of IMP at 48 h postmortem than did the wild-type fish. The nt5eb mutants also exhibited higher IMP contents compared to the wild types, but the increase was less than that in the nt5ea mutants. Our results demonstrated that nt5e is an important regulator of IMP levels in skeletal muscle and that its loss of function was effective in maintaining IMP content.

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Physiological and biochemical characteristics of laboratory induced mutants of Botrytis cinerea with resistance to fluazinam

Shao

Ren

Chen

2015

Pesticide Biochemistry and Physiology

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ATP depletion inhibits retraction of chromatophores induced by GABA in cephalopod Todarodes pacificus

Cited by 3 publications

References 18 publications

Targeted deletion of ecto-5′-nucleotidase results in retention of inosine monophosphate content in postmortem muscle of medaka (Oryzias latipes)

Targeted deletion of ecto-5′-nucleotidase results in retention of inosine monophosphate content in postmortem muscle of medaka (Oryzias latipes)

Targeted deletion of ecto-5′-nucleotidase results in retention of inosine monophosphate content in postmortem muscle of medaka (Oryzias latipes)

Physiological and biochemical characteristics of laboratory induced mutants of Botrytis cinerea with resistance to fluazinam

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