ATP-independent DNA strand transfer catalyzed by protein(s) from meiotic cells of the yeast Saccharomyces cerevisiae.

Sugino, Akio; Nitiss, John L.; Resnick, Michael A.

doi:10.1073/pnas.85.11.3683

Cited by 72 publications

(36 citation statements)

References 15 publications

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“…PPR2 is not an essential gene, and disruption mutants are characterized by sensitivity to nucleotidedepleting drugs such as 6-azauracil and mycophenolic acid (Exinger and Lacroute 1992). Such mutants also exhibit some effects on stationary phase morphology (Christie 1995), a resistance to oxidative damage (Seidel 2002), a possible effect on meiotic recombination (Sugino et al 1988), and a sensitivity to microtubuledestabilizing drugs (Malagon et al 2004).…”

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confidence: 99%

Genetic Interactions Between TFIIF and TFIIS

et al. 2006

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The eukaryotic transcript elongation factor TFIIS is encoded by a nonessential gene, PPR2, in Saccharomyces cerevisiae. Disruptions of PPR2 are lethal in conjunction with a disruption in the nonessential gene TAF14/TFG3. While investigating which of the Taf14p-containing complexes may be responsible for the synthetic lethality between ppr2D and taf14D, we discovered genetic interactions between PPR2 and both TFG1 and TFG2 encoding the two larger subunits of the TFIIF complex that also contains Taf14p. Mutant alleles of tfg1 or tfg2 that render cells cold sensitive have improved growth at low temperature in the absence of TFIIS. Remarkably, the amino-terminal 130 amino acids of TFIIS, which are dispensable for the known in vitro and in vivo activities of TFIIS, are required to complement the lethality in taf14D ppr2D cells. Analyses of deletion and chimeric gene constructs of PPR2 implicate contributions by different regions of this N-terminal domain. No strong common phenotypes were identified for the ppr2D and taf14D strains, implying that the proteins are not functionally redundant. Instead, the absence of Taf14p in the cell appears to create a dependence on an undefined function of TFIIS mediated by its N-terminal region. This region of TFIIS is also at least in part responsible for the deleterious effect of TFIIS on tfg1 or tfg2 cold-sensitive cells. Together, these results suggest a physiologically relevant functional connection between TFIIS and TFIIF.

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confidence: 99%

Genetic Interactions Between TFIIF and TFIIS

et al. 2006

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“…Previously, we have described the partial purification of strand exchange activities, termed recombinase fractions, from the human B lymphoblastoid cell line RPMI 1788 (Hsieh et al 1986), HeLa cells (Hsieh and Camerini-Otero 1989), and Drosophila melanogaster embryos (Eisen and Camerini-Otero 1988). DNA strand exchange or pairing activities from mitotic and meiotic cells of Saccharomyces cerevisiae (Kolodner et al 1987;Sugino et al 1988;Halbrook and McEntee 1989), Drosophila melanogaster embryos (McCarthy et al 1988), and human cells (Fishel et al 1988) have been reported by others.…”

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confidence: 99%

Pairing of homologous DNA sequences by proteins: evidence for three-stranded DNA.

Hsieh¹,

Camerini-Otero²,

Camerini‐Otero³

1990

Genes Dev.

140

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We show that recombinases form joint molecules over very short regions of homology. When these molecules are deproteinized the three strands are in a structure that is surprisingly resistant to dissociation by branch migration, even at elevated temperatures. The joint molecules dissociate at temperatures comparable to those required to melt DNA duplexes of the same length and sequence. We also show that nonenzymatically formed structures of the same length and sequence, which have a free third strand ready to branch migrate, dissociate at much lower temperatures. These results provide compelling evidence that the three DNA strands in the region of pairing are hydrogen bonded to each other. Our observations suggest that such a novel three-stranded DNA molecule, or a structure very similar to it, may be the intermediate in general recombination that is used in the recognition of sequence homology. We discuss some of the structural features implicit in this molecule containing any base sequence and compare them with those manifest in true DNA triple helices containing special sequence motifs.

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“…Each step may be carried out by an enzyme but it is possible that a cell may have two enzymes for the same steps as a safeguard. Also, the entire recombination process is operated by multienzymatic processes, as suggested by the presence of both ATP-dependent recA-like protein (m-rec) (1) and ATP-independent recA-like protein (mAi-rec) (14,15) in meiotic cells and the presence of meiotic recombination enzymes in meiotic cells and somatic recombination enzyme in somatic cells (1). These observations strongly support the presence of a multi-system functioning in various types of DNA recombination.…”

Section: Resultsmentioning

confidence: 65%