ATP synthase oligomerization: From the enzyme models to the mitochondrial morphology

Habersetzer, Johan; Ziani, Widade; Larrieu, Isabelle; Stinès-Chaumeil, Claire; Giraud, Marie‐France; Brèthes, Daniel; Dautant, Alain; Paumard, Patrick

doi:10.1016/j.biocel.2012.05.017

Cited by 75 publications

(70 citation statements)

References 74 publications

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“…Moreover, deletion of subunit e and g of ATP synthase F1 in yeast leads to no dimerization and oligomerization where the classical cristae shape did not exist; this was replaced by onion-shape mitochondria, although the enzyme activity is fully functional. These data support the idea of the correlation between the organization of ATP synthase and mitochondrial morphology (Habersetzer et al 2013). Two previous publications suggested that ATP5B might be involved in mitochondrial dynamics.…”

Section: Introductionsupporting

confidence: 90%

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ATP5B regulates mitochondrial fission and fusion in mammalian cells

Seo

Lee

Chung

et al. 2016

Animal Cells and Systems

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Mitochondria are essential organelles that produce ATP and regulate cell growth, proliferation, and cell death. To maintain homeostasis, fusion and fission of mitochondria must be strictly regulated. Even though oligomerization of ATP synthase could affect the mitochondrial morphology, the exact mechanism is not clear. We confirmed that structure and function of ATP5B, which is a major component of the catalytic center of ATP synthase complexes, are closely connected to the mitochondrial morphology. ATP5B itself can enhance elongation of mitochondria. Moreover, mutations of the threonine residue at β-barrel domain, and the serine residue at nucleotidebinding domain of ATP5B, produce the opposite effect on the fission and fusion of mitochondrial networks. Here, we demonstrate that ATP5B is clearly involved in the mechanism of regulation for mitochondrial fusion and fission in mammalian cells. ARTICLE HISTORY

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Section: Introductionsupporting

confidence: 90%

“…The ATP synthase complex is constituted by F 1 part (subunits a, b, g, d, and 1) and F parts (subunits b, F6, d, f, a, A6L, and OSCP), and uses the electrochemical proton gradient produced by the electron transport chain complexes, to synthesize ATP (Habersetzer et al 2013).…”

Section: Introductionmentioning

confidence: 99%

ATP5B regulates mitochondrial fission and fusion in mammalian cells

Seo

Lee

Chung

et al. 2016

Animal Cells and Systems

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“…Moreover, using digitonin extraction, hr CN PAGE, in-gel ATPase activity staining and native immunoblotting, we show that ecto-F 0 F 1 complexes have a similar molecular mass to the mitochondrial ATP synthase monomers and preserve their sensitivity to the inhibitor oligomycin. Detection of only monomers in PM may be a consequence of the low concentration of ecto-F 0 F 1 complexes unable to form polymers, but it is consistent with the uniquely mitochondrial role of ATP synthase dimers to help formation of cristae (Habersetzer et al 2013), which are absent in lipid rafts (Gorai et al 2012).…”

Section: Discussionmentioning

confidence: 81%

Ectopic F0F1 ATP synthase contains both nuclear and mitochondrially-encoded subunits

Kumar

Spolaore

Harris

et al. 2013

J Bioenerg Biomembr

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“…One of the possibilities of MA-5 acting mechanisms is the effect on the oligomerization of ATP synthase. The oligomerization of ATP synthase is essential for the maintenance of cristae junctions (Habersetzer et al 2013). In addition, the oligomerization of ATP synthase does increase the local pH gradient and membrane potential and optimizes ATP synthesis without changing whole mitochondrial membrane potential (Strauss et al 2008).…”

Section: Discussionmentioning

confidence: 99%

Mitochonic Acid 5 (MA-5), a Derivative of the Plant Hormone Indole-3-Acetic Acid, Improves Survival of Fibroblasts from Patients with Mitochondrial Diseases

Suzuki

Yamaguchi

Kikusato

et al. 2015

Tohoku J. Exp. Med.

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Mitochondria are key organelles implicated in a variety of processes related to energy and free radical generation, the regulation of apoptosis, and various signaling pathways. Mitochondrial dysfunction increases cellular oxidative stress and depletes ATP in a variety of inherited mitochondrial diseases and also in many other metabolic and neurodegenerative diseases. Mitochondrial diseases are characterized by the dysfunction of the mitochondrial respiratory chain, caused by mutations in the genes encoded by either nuclear DNA or mitochondrial DNA. We have hypothesized that chemicals that increase the cellular ATP levels may ameliorate the mitochondrial dysfunction seen in mitochondrial diseases. To search for the potential drugs for mitochondrial diseases, we screened an in-house chemical library of indole-3-acetic-acid analogs by measuring the cellular ATP levels in Hep3B human hepatocellular carcinoma cells. We have thus identified mitochonic acid 5 (MA-5), 4-(2,4-difluorophenyl)-2-(1H-indol-3-yl)-4-oxobutanoic acid, as a potential drug for enhancing ATP production. MA-5 is a newly synthesized derivative of the plant hormone, T. Suzuki et al. 226

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ATP synthase oligomerization: From the enzyme models to the mitochondrial morphology

Cited by 75 publications

References 74 publications

ATP5B regulates mitochondrial fission and fusion in mammalian cells

ATP5B regulates mitochondrial fission and fusion in mammalian cells

Ectopic F0F1 ATP synthase contains both nuclear and mitochondrially-encoded subunits

Mitochonic Acid 5 (MA-5), a Derivative of the Plant Hormone Indole-3-Acetic Acid, Improves Survival of Fibroblasts from Patients with Mitochondrial Diseases

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