ATPase, antigens detected by monoclonal antibodies, ultrastructural characters: which is the best marker for langerhans cells?

Panfilis, Giuseppe De

doi:10.1007/bf00417343

Cited by 32 publications

(11 citation statements)

References 29 publications

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“…By light microscopy they are identified using enzyme histochemical techniques, such as adenosine triphosphatase (ATPase). However, the most specific surface marker for human cutaneous LC has been shown to be the thymocyte antigen (HTA-1) defined by the mouse monoclonal antibody OKT6 (6). Especially the presence of HLA-DR/antigens has been utilized in imtnunohistological staining procedures for LC (2).…”

Section: Discussionmentioning

confidence: 99%

“…Especially the presence of HLA-DR/antigens has been utilized in imtnunohistological staining procedures for LC (2). However, the most specific surface marker for human cutaneous LC has been shown to be the thymocyte antigen (HTA-1) defined by the mouse monoclonal antibody OKT6 (6). We therefore applied the OKT6 antibody m indirect lmmunohistologic techniques in order to \dsualize cutaneous LC.…”

Section: Discussionmentioning

confidence: 99%

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Epidermal Langerhans Cells in Patients with Primary Sjögren's Syndrome

Oxholm

Oxholm²,

Manthorpe

1986

Allergy

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IgG-binding to the surface of Langerhans cells (LC) is a feature of primary Sjögren's syndrome and might be associated with qualitative and quantitative defects in LC. Fifteen patients with primary Sjögren's syndrome and 15 healthy controls had a punch biopsy performed from clinically unaffected skin. LC were enumerated in epidermal sheets by an immunoperoxidase technique, as well as demonstrated by immunofluorescence in vertical sections, which were furthermore examined for in vivo deposits of immunoglobulins, fibrinogen and C3. A significant (P less than 0.05) reduction in number of LC, measured on epidermal sheets, was found in patients as compared with controls. Examination of vertical sections did not reveal abnormalities in morphology or localisation of epidermal LC. Intraepidermal IgG deposits were demonstrated in 7/15 patients. In vivo deposits were not found in any of the controls. The density of epidermal LC was not correlated to the presence of intraepidermal IgG deposits.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Epidermal Langerhans Cells in Patients with Primary Sjögren's Syndrome

Oxholm

Oxholm²,

Manthorpe

1986

Allergy

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“…The OKT6 antibody was chosen in the staining procedure for LC, as it has been shown to be the most specific surface-marker for cutaneous LC (8). It is important always to examine skin biopsies from the same area when inter-individual comparisons of LS are performed.…”

Section: Discussionmentioning

confidence: 99%

Immunohistological Skin Investigations in Patients With the Acquired Immune Deficiency Syndrome

Oxholm

Helweg-Larsen

Permin

1986

Acta Pathologica Microbiologica Scandinavica Series A :Patholog

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Oxholm, P., Helweg-Larsen, S. & Permin, H. Immunohistological skin investigations in patients with the acquired immune deficiency syndrome. Acta path. rnicrobiol. immunol. scand. Sect. A, 94: 1 13-1 16, 1986. Twenty-one male patients with acquired immune deficiency syndrome (AIDS), 6 male patients with AIDS-related complex (ARC) and 23 controls' had a punch biopsy taken from clinically unaffected skin of the buttock. Vertical skin sections were examined by immunofluorexence for in vivo deposits of immunoglobulins (Ig) and other plasma proteins, as well as for morphology and distribution of Langerhans cells (LC). Deposits of IgM in the dermo-epidermal junction zone (DEJ) were found in one patient with ARC. Apart from this single finding, no in vivo deposits of IgG, IgM, IgA, IgD, IgE, complement Clq and C3, fibrinogen or albumin were demonstrated in epidermis, DEJ or dermal vessel walls, either in patients or in controls. Epidermal LC were more superficially situated in patients as compared to controls. No differences in number and localisation of dermal LC were seen. We concluded that it was not possible to confirm a recent report of in vivo Ig deposits in the epidermis of patients with AIDS. The abnormalities observed in epidermal LC should be interpreted together with the recent finding of reduced numbers of epidermal LC; they thus support the hypothesis that LC are involved in the pathological processes in AIDS.

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“…Dendritic cells seem to be a common feature in the dermal infiltrate of mycosis fungoides (MF) (Jimbow, Chiba & Horikoshi 1982); when, as often occurs in this infiltrate, dendritic cells contain Birbeck granules, they are identified as Langerhans cells (De Panfilis 1983). Dendritic cells have also been described in the dermal infiltrate of four patients with Sezary syndrome (SS) (Goos 1976, Van der Loo et al 1979Iwahara & Hashimoto 1984), but these authors do not state whether Birbeck granules were found.…”

Section: Introductionmentioning

confidence: 99%

Dendritic cells in the dermal infiltrate of Sézary syndrome

et al. 1986

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The dermal infiltrates of four patients with the Sézary syndrome were studied by electron microscopy and the data were evaluated quantitatively. The nuclear contour index of lymphocytes was calculated, and many tumour cells had an index greater than 6.5. Dendritic cells were found in all cases. The dendritic cells contained smooth and rough endoplasmic reticulum, moderately well-developed Golgi apparatus, scanty lysosomes and many thin and intermediate filaments; their surface was scalloped with numerous vesicles. Birbeck granules were not found in the cytoplasm of dendritic cells. Dendritic cells comprised 24% of infiltrating cells and were interspersed with lymphocytes; 75% of the lymphocytes were in contact with dendritic cells; 35% of the lymphocytes in contact with dendritic cells had a nuclear contour index higher than 6.5 and 76% had a nuclear contour index higher than 5. The data strongly suggest a functional relationship between lymphocytes and dendritic cells in the dermal infiltrate of Sézary syndrome. They are discussed in relation to the hypothesis that the disease is a consequence of chronic immune stimulation.

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ATPase, antigens detected by monoclonal antibodies, ultrastructural characters: which is the best marker for langerhans cells?

Cited by 32 publications

References 29 publications

Epidermal Langerhans Cells in Patients with Primary Sjögren's Syndrome

Epidermal Langerhans Cells in Patients with Primary Sjögren's Syndrome

Immunohistological Skin Investigations in Patients With the Acquired Immune Deficiency Syndrome

Dendritic cells in the dermal infiltrate of Sézary syndrome

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