ATpases and Membrane Properties in Relation to Ecological Differences

Kylin, Anders; Sommarin, Marianne

doi:10.1007/978-1-4613-2177-4_32

Cited by 4 publications

(1 citation statement)

References 8 publications

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“…such as the vacuole or the endoplasmic reticulum. Calcium efflux is via a Ca 2+ ATPasc, but there is evidence that there is very little Ca 2+ ATPase activity in calcifuge species such as oat (Kylin andSommarin. 1986: Monestiez et al.…”

Section: Resultsmentioning

confidence: 99%

Labeled Sodium (22Na+) Uptake and Translocation in Rabbiteye Blueberry Exposed to Sodium Chloride and Supplemental Calcium

Wright¹,

Patter²,

Drew³

1995

jashs

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`Tifblue' rabbiteye blueberry (Vaccinium ashei Reade) plants grown in complete nutrient solution were supplied with 25 mm NaCl and with either 0, 3, or 10 mm supplemental Ca2+ added as a mixture of the sulfate, nitrate, and chloride salts. Uptake and partitioning of 22Na+ into leaves, stems, and roots from labeled nutrient solutions and subsequent translocation in the absence of additional labeled Na+ (pulse-chase experiment) was determined. Plants were harvested at intervals following the uptake period. At 28 days, plants supplied with 10 mM Ca2+ accumulated 35% to 68% more 22Na+ in the 3rd-18th leaves from the apex and in herbaceous stems than plants not supplied with Ca2+. Leaf Na+ concentrations followed a similar trend. There was a preferential translocation of Na+ to the shoots compared to the other plant parts, which accounted for 30% of the total plant Na+ immediately following the uptake period and 15% at 28 days. Blueberry plants supplied with 3 mm Ca2+ did not have greater leaf or stem 2Na+ concentrations or total Na+ content than plants not supplied with Ca2+. The 2Na+ content did not decrease with 3 mM Ca2+ treatments. It is proposed that the failure of a high level (10 mm) of Ca2+ to protect against Na+ uptake and translocation to the herbaceous shoots is due to metabolic dysfunction. Abnormally high levels of Ca2+ in the cytoplasm may lead to an inability to sequester or exclude Na+.

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Section: Resultsmentioning

confidence: 99%

Labeled Sodium (22Na+) Uptake and Translocation in Rabbiteye Blueberry Exposed to Sodium Chloride and Supplemental Calcium

Wright¹,

Patter²,

Drew³

1995

jashs

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A Critical Evaluation of Markers Used in Plasma Membrane Purification

Widell

Larsson

1990

The Plant Plasma Membrane

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Purification of endoplasmic reticulum from wheat roots and shoots (Triticum aestivum). Comparison of their blue light‐sensitive redox components with those of highly purified plasma membrane

Widell

Sommarin

1991

Physiologia Plantarum

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Plasma mebranes (PM) and endoplasmic reticulum (ER) were prepared from 4.5‐day‐old, light‐grown wheat (Triticum aestivum L. cv. Drabant) shoots and roots, using phase partitioning for the PM, which yields very pure PM preparations, and sucrose gradient centrifugation for the ER. Also the ER fractions were highly purified, being totally free from mitochondria (cytochrome c oxidase, EC 1.9.3.1), PM (glucan synthase II, EC 2.4.1.34) and thylakoid membranes (chlorophyll), and with a low content of tonoplast (nitrate‐sensitive ATPase) and Golgi (latent IDPase). Sodium dodecyl sulphate polyacrylmide gel electrophoresis of root ER resulted in a similar polypeptide pattern as for shoot ER, but very different from the crude fraction from which the ER fractions were purified, also indicative of a high purity. The PM and ER preparations were compared with respect to their blue light‐sensitive flavoprotein‐cytochrome b. About half of the dithionite‐reducible cytochrome b of shoots (PM as well as ER) and root PM was light‐sensitive, compared to only 10–20% of that of root ER. Shoot PM needed 2–3 times less light compared to the other membranes, for half saturation of the reaction. NADH‐cytochrome c reductase activity was found with all four membrane fractions, with ER having activities 5–10 times higher than PM. The relevance of photoreducible components in the PM and the ER is discussed in connection with blue light photobiology.

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ATpases and Membrane Properties in Relation to Ecological Differences

Cited by 4 publications

References 8 publications

Labeled Sodium (22Na+) Uptake and Translocation in Rabbiteye Blueberry Exposed to Sodium Chloride and Supplemental Calcium

Labeled Sodium (22Na+) Uptake and Translocation in Rabbiteye Blueberry Exposed to Sodium Chloride and Supplemental Calcium

A Critical Evaluation of Markers Used in Plasma Membrane Purification

Purification of endoplasmic reticulum from wheat roots and shoots (Triticum aestivum). Comparison of their blue light‐sensitive redox components with those of highly purified plasma membrane

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