ATR/FTIR characterization of Steinernema glaseri and Heterorhabditis indica

San‐Blas, Ernesto; Guerra, Mayamarú; Portillo, Edgar; Estéves, Ivan; Cubillán, Néstor; Alvarado, Ysaías J.

doi:10.1016/j.vibspec.2011.07.008

Cited by 17 publications

(13 citation statements)

References 42 publications

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“…The band at 1124 cm -1 was due to C-O, C-C stretching, C-O-H, C-O-C deformations of glycogen (Chiriboga et al 1988, Diem et al 2004). The region around 1074 cm −1 was assigned to C-C, C-O, C-O-C stretching vibrations of carbohydrates (San-Blas et al 2011). This evidence indicated external oxidative stress damaged the nematode cuticle.…”

Section: Mechanism Of Nematode Deactivationmentioning

confidence: 86%

“…Fig. 5c shows that vibrations at 1660, 1548 and 3469 cm -1 were assigned to amide I, amide II and asymmetric stretching N-H bonds of proteins (Movasaghi et al 2008, San-Blas et al 2011). Vibrations at 2925 and 2856 cm -1 were assigned to asymmetric CH 2 and symmetric CH 3 stretches of the lipids (Bouyan f et al 2018), conforming to literature reports that the main biochemical components of the cuticle are proteins, lipids and water (Xue et al 2012).…”

Section: Mechanism Of Nematode Deactivationmentioning

confidence: 99%

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Deactivation of Caenorhabditis elegans nematodes in drinking water by PMS/UV-C: efficiency and mechanisms

Chen

et al. 2021

Environ Sci Pollut Res

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The occurrence an d infestations of chlorine-resistant invertebrates in drinking water distributions have attracted concerns on water quality in China, making effective deactivation imperative. This study presents a no vel strategy for nematode ( Caenorhabditis elegans ) deactivation using peroxymonosulfate (PMS)/UV-C. The results indicated that 100% deactivation e ciency was obtained under optimal conditions. An acidic pH and 0.25 mg/L Fe(II) were bene cial to the PMS/UV-C-triggered deactivation of nematodes. A mechanism study demonstrated that was activated by UV-C to produce • OH and • SO 4 -, which resulted in oxidative stress and stimulated the occurrence of cell apoptosis, leading to nematode deactivation. The results reveal PMS/UV-C as an alternative to chlorination in water treatment plants (WTP) or an emergency application when chlorine-resistant invertebrates breed in a second-supply water tank, is a promising strategy for disinfection. This approach afforded the advantages of avoiding the production of chlorine disinfection by-products (DBP) and greater e cacy of nematode deactivation. This work will provide ideas for on-going research efforts into chlorine-resistant invertebrate deactivation, and eventually achieve the direct drinking of municipal tap water.

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Section: Mechanism Of Nematode Deactivationmentioning

confidence: 86%

Section: Mechanism Of Nematode Deactivationmentioning

confidence: 99%

Deactivation of Caenorhabditis elegans nematodes in drinking water by PMS/UV-C: efficiency and mechanisms

Chen

et al. 2021

Environ Sci Pollut Res

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“…The pretreatment of spectra was carried out following methods outlined by San-Blas et al (2011). This included ATR and baseline corrections, scaling between 0 and 1, and the 5-point adjacent averaging second derivative spectrum calculation using the IR Solution Software (Shimadzu).…”

Section: Fourier-transformed Mid-infrared Spectroscopy (Ftir)mentioning

confidence: 99%

“…Each decalcified and dried sample was placed directly over the ATR plate, and the pressure clamp was applied with the manufacturing default settings. The total area analyzed for each sample varied from 5 to 7 cm 2 ; the analysis of the gross composition of tissues is therefore on the scale of centimeters for the corals.The pretreatment of spectra was carried out following methods outlined by San-Blas et al (2011). This included ATR and baseline corrections, scaling between 0 and 1, and the 5-point adjacent averaging second derivative spectrum calculation using the IR Solution Software (Shimadzu).…”

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confidence: 99%

Fourier-transformed infrared spectroscopy: a tool to identify gross chemical changes from healthy to yellow band disease tissues

Guerra¹,

López²,

Estéves³

et al. 2014

Dis. Aquat. Org.

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Yellow band disease (YBD) is a common and wide-spread Caribbean syndrome that affects the genus Orbicella, a group of species that constitute the framework of Caribbean coral reefs. Previous studies have shown that the structure and function of bacterial assemblages vary between healthy tissues and YBD lesions; however, how the molecular composition of tissues varies as tissues transition from healthy to YBD has not been determined before. The present study provides the first survey of macromolecules found from healthy (H), apparently healthy (AH), transition (TR) and YBD tissues of Orbicella faveolata. For this, we used Fourier-transformed mid-infrared spectroscopy (FTIR) to compare absorption profiles as a proxy for the gross molecular composition of decalcified H, AH and YBD tissues. We found a significantly higher level of infrared absorption for bands assigned to lipids in H tissues compared to YBD tissues, suggesting that lipid compounds are more abundant in compromised tissues in relation to other macromolecules. We also found a lower level of intensity of bands assigned to carbohydrates and proteins in YBD tissues, compared to H and AH tissues. A similar pattern was observed for phospholipidic compounds in relation to fatty acids. This study is the first to show that healthy and YBD-compromised tissues have different infrared absorption profiles, suggesting that alterations in the biochemical composition occur during pathogenesis. Future studies should focus on determining the actual concentration of these compounds in H, AH, TR and YBD tissues and on testing the role of translocation of photoassimilates from H tissues and/or from endolithic algae to YBD tissues. KEY WORDS: Coral diseases · Yellow band disease · Infrared spectroscopy · Tissue composition Resale or republication not permitted without written consent of the publisherDis Aquat Org 107: [249][250][251][252][253][254][255][256][257][258] 2014 Among the diseases described from the Caribbean (Green & Bruckner 2000), yellow band (YBD) is arguably the one with the largest detrimental and longest lasting effects for coral reef health in the region (Cróquer et al. 2012). This syndrome tends to be persistent (Bruckner & Bruckner 2006), and it affects all species in the genera Orbicella and Montastraea e.g. O. annularis, O. faveolata, O. franksi and M. cavernosa), which are the dominant reef-building corals in the region (for specific examples, see Cortés 2003). Furthermore, YBD compromises the fecundity of M. faveolata ), produces significant mortality on larger colonies and has contributed to an overall reduction of live coral cover (Bruckner & Bruckner 2006) over the past decades. Combined, these impacts significantly affect the population size of Orbicella species, and therefore, it has been hypothesized that YBD alone might reduce reef complexity throughout the Caribbean (Bruckner & Bruckner 2006). While other Caribbean diseases such as white plague, white band, white pox and patchy necrosis may reduce coral cover rapidly, infected...

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“…By passing a beam of infrared light through the sample, this technique allows chemical bonds and groups to be identified by their vibrational modes after IR excitation. Therefore, a unique spectral fingerprint can be recorded for any chemical compound, tissue, or the whole organism (San-Blas et al, 2011). This technique offers a series of benefits such as speed of data acquisition, high sensitivity, low costs, and non-invasive/ non-destructive sampling in many cases (i.e., using handheld FTIR-ATR spectrometers; Durak and Depciuch, 2020).…”

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confidence: 99%

The use of infrared spectroscopy and machine learning tools for detection ofMeloidogyneinfestations

San‐Blas¹,

Paba

Cubillán

et al. 2020

Plant Pathology

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Plant parasitic nematodes are generally soilborne pathogens that attack plants and cause economic losses in many crops. The infested plants show nonspecific symptoms or, often, are symptomless; therefore, diagnosis is performed by taking soil and root tissue samples. Here, we show that a combination of different infrared spectra analysis and machine learning algorithms can be used to detect plant parasitic nematode infestations before symptoms become visible, using leaves instead of roots and soil as samples. We found that tomato and guava plants infested with Meloidogyne enterorlobii produced different spectral patterns compared to uninfested plants. Using partial spectra from 1,450 to 900/cm as the "fingerprint region", principal component analyses indicated that after 5 (tomatoes) or 8 weeks (guava), plants with no visible symptoms of infestations were positively diagnosed. To improve the early detection response, we used machine learning modelling. A support vector machine (SVM) was used to obtain more robust, accurate models. The SVM model contained 34 support vectors, 17 for each level. The overall performance of the model was >97% and the total accuracy was significantly higher, demonstrating the absence of chance prediction. The best prediction of infestation was obtained at the second and fourth weeks for tomatoes and guavas, respectively, reducing the diagnostic time by half. The combined application of these techniques reduces the processing time from field to laboratory and shows enormous advantages by avoiding root and soil sampling.

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ATR/FTIR characterization of Steinernema glaseri and Heterorhabditis indica

Cited by 17 publications

References 42 publications

Deactivation of Caenorhabditis elegans nematodes in drinking water by PMS/UV-C: efficiency and mechanisms

Deactivation of Caenorhabditis elegans nematodes in drinking water by PMS/UV-C: efficiency and mechanisms

Fourier-transformed infrared spectroscopy: a tool to identify gross chemical changes from healthy to yellow band disease tissues

The use of infrared spectroscopy and machine learning tools for detection ofMeloidogyneinfestations

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