ATR–FTIR Spectroscopic Investigation of Imipenem-Susceptible and -ResistantPseudomonas aeruginosaIsogenic Strains

Sockalingum, Ganesh D.; Bouhedja, W.; Pina, P.; Allouch, P.; Mandray, C.; Labia, Roger; Millot, Jean-Marc; Manfait, Michel

doi:10.1006/bbrc.1997.6263

Cited by 75 publications

(35 citation statements)

References 23 publications

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“…FTIR spectroscopy is increasingly being used for the identification of microorganisms at the species level, but it has been proven to have the potential to discriminate between strains of the same species (9,17,18,20). In this study, we aimed to investigate the capability of this technique to compare strains of C. albicans, a widely occurring yeast and opportunistic pathogen, in a clinical setting.…”

Section: Resultsmentioning

confidence: 99%

Use of Fourier-Transform Infrared Spectroscopy for Typing of Candida albicans Strains Isolated in Intensive Care Units

et al. 2003

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Comparative studies of Candida albicans strains are essential for proving cross-infections in epidemiological investigations. Typing of C. albicans strains is mainly based on genotypic methods. Fourier-transform infrared (FTIR) spectroscopy is described in this study as a novel phenotypic approach to the typing of C. albicans. The first step in the approach was the standardization of sample preparation (culture conditions and sampling parameters) and acquisition and classification parameters (spectral acquisition, spectral window selection, classification algorithm, and heterogeneity threshold). The second step consisted of validating the established parameters with a set of 79 strains of C. albicans isolated over 4 months from nine patients hospitalized in two intensive care units. Strains were isolated from multiple anatomical sites with repeated sampling. FTIR spectroscopy results were compared to randomly amplified polymorphic DNA (RAPD) results; this analysis showed that the amplification patterns of strains isolated from a given patient were identical and that different patients had different profiles. FTIR spectroscopy data were analyzed by hierarchical clustering performed with the second-derivative spectra. This classification revealed nine groups, one per patient. Only one spectrum out of 79 was misclassified by the FTIR spectroscopy method. RAPD and FTIR spectroscopy results were in good agreement, showing that, when nosocomial candidiasis transmission is suspected and urgent information is needed, this technique may be useful as a quick identification tool to give solid clues before confirmation by a genotypic method.Fungal infections occur mainly in immunodeficient patients or those harboring several risk factors, such as broad-spectrum antibiotic therapy, digestive surgery, catheter implantation, or graft transplantation. Thus, intensive care unit (ICU) patients are high-risk patients since they generally have several of these factors. Candida spp. constitute the third or fourth most common cause of nosocomial infections in ICUs, according to data from the National Nosocomial Infections Surveillance System and the European Prevalence of Infection in Intensive Care (1,22). Candida albicans is generally responsible for up to 50 to 70% of the infections, and its endogenous origin is generally implicated (3,7,21,23). However, cases of exogenous contamination have been described (4,6,13,14,16). Demonstration of the exogenous origin of a contaminant pathogen is based on the identification and comparison of strains. Among the different techniques used, genotypic methods are favored over phenotypic methods. Randomly amplified polymorphic DNA (RAPD) and other DNA fingerprinting methods, PCR-restriction fragment length polymorphism analysis, multilocus enzyme electrophoresis, DNA sequencing, and pulsed-field gel electrophoresis are generally used to check strain identity. These techniques are successful but require time, expensive consumables, and highly trained staff to be performed adequately.In this study...

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Section: Resultsmentioning

confidence: 99%

Use of Fourier-Transform Infrared Spectroscopy for Typing of Candida albicans Strains Isolated in Intensive Care Units

et al. 2003

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“…Its use for bacterial studies is simpler since no suspension or dehydration of the sample is required. Recently, associated with chemometrics methods, it was successfully used to discriminate isogenic strains of P. aeruginosa with varying degrees of resistance to imipenem [9].…”

Section: Introductionmentioning

confidence: 99%

ATR‐FTIR spectroscopic investigation of E. coli transconjugants β‐lactams‐resistance phenotype

et al. 1997

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Hyphenation of attenuated total reflection Fourier transform infrared spectroscopy and cluster analysis has been used to characterise a susceptible Escherichia coli K12 strain and the transeonjugants TEM-1, TEM-2, TEM-3, SHV-2, SHV-3, SHV-4. A good discrimination of the susceptible strain from the transeonjugants was obtained. Although a limited success was achieved in the differentiation of SHV and TEM phenotypes in general, results obtained with TEM-2 and SHV-3 were convincing. Spectral differences observed are ascribed to the global effects of the conjugation process, particularly their repercussions in the nucleic acids and carbohydrate absorbing regions, rather than to P-lactamase point-mutations.

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“…Noteworthy, bacterial typing at different taxonomic levels, detection of contaminations, [7][8][9] and antibiotic resistance [10][11][12][13][14][15] were among the most reported. These applications were mostly selected given the limitations (e.g., cost per analysis) of the gold standard molecular biology techniques for these purposes.…”

Section: Infrared Spectroscopy and Bacteriamentioning

confidence: 99%

An Overview of the Evolution of Infrared Spectroscopy Applied to Bacterial Typing

Quintelas

Ferreira

Lopes

et al. 2017

Biotechnology Journal

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The sustained emergence of new declared bacterial species makes typing a continuous challenge for microbiologists. Molecular biology techniques have a very significant role in the context of bacterial typing, but they are often very laborious, time consuming, and eventually fail when dealing with very closely related species. Spectroscopic-based techniques appear in some situations as a viable alternative to molecular methods with advantages in terms of analysis time and cost. Infrared and mass spectrometry are among the most exploited techniques in this context: particularly, infrared spectroscopy emerged as a very promising method with multiple reported successful applications. This article presents a systematic review on infrared spectroscopy applications for bacterial typing, highlighting fundamental aspects of infrared spectroscopy, a detailed literature review (covering different taxonomic levels and bacterial species), advantages, and limitations of the technique over molecular biology methods and a comparison with other competing spectroscopic techniques such as MALDI-TOF MS, Raman, and intrinsic fluorescence. Infrared spectroscopy possesses a high potential for bacterial typing at distinct taxonomic levels and worthy of further developments and systematization. The development of databases appears fundamental toward the establishment of infrared spectroscopy as a viable method for bacterial typing.

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ATR–FTIR Spectroscopic Investigation of Imipenem-Susceptible and -ResistantPseudomonas aeruginosaIsogenic Strains

Cited by 75 publications

References 23 publications

Use of Fourier-Transform Infrared Spectroscopy for Typing of Candida albicans Strains Isolated in Intensive Care Units

Use of Fourier-Transform Infrared Spectroscopy for Typing of Candida albicans Strains Isolated in Intensive Care Units

ATR‐FTIR spectroscopic investigation of E. coli transconjugants β‐lactams‐resistance phenotype

An Overview of the Evolution of Infrared Spectroscopy Applied to Bacterial Typing

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