ATR spectroscopy of thin films

Fringeli, U. P.; Baurecht, Dieter; Siam, Monira; Reiter, Gerald; Schwarzott, M.; Bürgi, Thomas; Brüesch, P.

doi:10.1016/b978-012512908-4/50023-0

Cited by 19 publications

(41 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…They are used in this case to establish the relation between the unit vector of a transition moment in the molecule fixed coordinate system (t-system, x t , y t , z t ) and the labor-coordinate system (x, y, z). For details see Fringeli et al (2002).…”

Section: Stationary Spectra Of Dmpcmentioning

confidence: 99%

Electric Field-Induced Changes in Lipids Investigated by Modulated Excitation FTIR Spectroscopy

Schwarzott

Baurecht

Naumann

et al. 2004

Biophysical Journal

Self Cite

View full text Add to dashboard Cite

The effect of electric fields on dry oriented multibilayers of dimyristoylphosphatidylcholine (DMPC) was investigated by transmission Fourier transform infrared electric field modulated excitation (E-ME) spectroscopy. A periodic rectangular electric potential (0-150 V, 1.25 Hz, 28.4 degrees C +/- 0.2 degrees C) was applied across the sample. To discriminate electric field-induced effects from possible temperature-induced effects resulting from a current flow (<1 pA) across the sample, corresponding temperature-modulated excitation (T-ME) measurements within the temperature uncertainty limits of +/-0.2 degrees C at 28.4 degrees C were performed. T-ME induced reversible gauche defects in the hydrocarbon chains, whereas E-ME resulted in reversible compression of dry DMPC bilayers. Periodic variation of the tilt angle of the hydrocarbon chains is suggested. The degree of absorbance modulation in the CH-stretching region was found to be in the order of 1:700, corresponding to a variation of the bilayer thickness of Deltaz = 0.0054 nm. Using a series connection of capacitors as equivalent circuit of the cell resulted in E = (1.2 +/- 0.7) x 10(7) V/m for the electric field in DMPC. Young's elasticity modulus of DMPC could be calculated to be E( perpendicular ) = 2.2 x 10(6) Pa +/- 1.8 x 10(6) Pa, which is in good agreement with published data obtained by electric field-dependent capacitance measurements.

show abstract

Section: Stationary Spectra Of Dmpcmentioning

confidence: 99%

Electric Field-Induced Changes in Lipids Investigated by Modulated Excitation FTIR Spectroscopy

Schwarzott

Baurecht

Naumann

et al. 2004

Biophysical Journal

Self Cite

View full text Add to dashboard Cite

show abstract

“…[16][17][18] In this setup, the IR light is reflected at the interface of a crystal (most commonly diamond, ZnSe, or Ge) and the sample. Since, in this process, an evanescent wave penetrates only a few mm into the sample, the protein film can be much thicker than in the transmittance cell.…”

Section: Methodsmentioning

confidence: 99%

Proteins in Action Monitored by Time‐Resolved FTIR Spectroscopy

2005

View full text Add to dashboard Cite

In the post genome era proteins coming into the focus of life sciences. X‐ray structure analysis and NMR spectroscopy are established methods to determine the geometry of proteins. In order to determine the molecular reaction mechanism of proteins, time‐resolved FTIR (trFTIR) difference spectroscopy emerges as a valuable tool. In this Minireview we describe the trFTIR difference spectroscopy and show its application on the light‐driven proton pump bacteriorhodopsin (bR), the photosynthetic reaction center and the GTPase Ras, which is crucial in signal transduction. The main principles of the technique are presented, including a summary of triggering techniques, scan modes and analysis.

show abstract

“…For the in situ observation of the inherent chemical modifications of the surface conditioned by responsive polymers, few methods have been described. Recently, the responsive behavior of as-deposited PNIPAAM layers was studied by attenuated total reflection spectroscopy, ATR-FT-IR. , An IR-based approach introduced by Fringeli, , reported as single-beam sample reference (SBSR) ATR spectroscopy, is a powerful extension of existing methodologies and was applied in this work. This technique converts a conventional single-beam spectrometer into a pseudo-double-beam mode by mechanically adjusting the vertical position of the internal reflection element (IRE) so that the sample and reference can be measured almost at the same time.…”

Section: Introductionmentioning

confidence: 99%

Dynamic Perspective on the Function of Thermoresponsive Nanopores from in Situ AFM and ATR-IR Investigations

et al. 2010

Self Cite

View full text Add to dashboard Cite

This article describes the morphological and chemical characterization of stimuli-responsive functionalized silicon surfaces provided in parallel by atomic force spectroscopy (AFM) and Fourier transform infrared spectroscopy (FT-IR) enhanced by the single-beam sample reference attenuated total reflection method (SBSR-ATR). The stimuli-responsive behavior of the surfaces was obtained by grafting-to in melt carboxyl-terminated poly-N-isopropylacryl amides (PNIPAAM) with different degree of polymerization (DP) on epoxide-functionalized silicon substrates. The unprecedented real time and in situ physicochemical insight into the temperature-triggered response of the densely packed superficial brushes allowed for the selection of a PNIPAAM with a specific DP as a suitable polymer for the fabrication of silicon membranes exhibiting switchable nanopores. The fabrication process combines the manufacture of nanoporous silicon surfaces and their subsequent chemical functionalization by the grafting-to in melt of the selected polymer. Then, relevant information was obtained in what concerns the chemical modifications behind the topographical changes that drive the functioning of PNIPAAM-based hybrid nanovalves as well as the timescale on which the opening and closing of the nanopores occur.

show abstract

ATR spectroscopy of thin films

Cited by 19 publications

References 39 publications

Electric Field-Induced Changes in Lipids Investigated by Modulated Excitation FTIR Spectroscopy

Electric Field-Induced Changes in Lipids Investigated by Modulated Excitation FTIR Spectroscopy

Proteins in Action Monitored by Time‐Resolved FTIR Spectroscopy

Dynamic Perspective on the Function of Thermoresponsive Nanopores from in Situ AFM and ATR-IR Investigations

Contact Info

Product

Resources

About