Atractylenolide-I Protects Human SH-SY5Y Cells from 1-Methyl-4-Phenylpyridinium-Induced Apoptotic Cell Death

Abstract

Oxidative stress and apoptosis are the major mechanisms that induce dopaminergic cell death. Our study investigates the protective effects of atractylenolide-I (ATR-I) on 1-methyl-4-phenylpyridinium (MPP+)-induced cytotoxicity in human dopaminergic SH-SY5Y cells, as well as its underlying mechanism. Our experimental data indicates that ATR-I significantly inhibits the loss of cell viability induced by MPP+ in SH-SY5Y cells. To further unravel the mechanism, we examined the effect of ATR-I on MPP+-induced apoptotic cell death characterized by an increase in the Bax/Bcl-2 mRNA ratio, the release of cytochrome-c, and the activation of caspase-3 leading to elevated levels of cleaved poly(ADP-ribose) polymerase (PARP) resulting in SH-SY5Y cell death. Our results demonstrated that ATR-I decreases the level of pro-apoptotic proteins induced by MPP+ and also restored Bax/Bcl-2 mRNA levels, which are critical for inducing apoptosis. In addition, ATR-I demonstrated a significant increase in the protein expression of heme-oxygenase in MPP+-treated SH-SY5Y cells. These results suggest that the pharmacological effect of ATR-I may be, at least in part, caused by the reduction in pro-apoptotic signals and also by induction of anti-oxidant protein.

Evaluation of potential neuroprotective effects of Vanillin against MPP+/MPTP-induced dysregulation of dopaminergic regulatory mechanisms in SH-SY5Y cells and a mouse model of Parkinson's disease

“…Here, we report the Van-mediated neuroprotection against MPP + -induced Parkinsonism in SH-SY5Y cells. In our study, MPP + administration resulted in a concentration-dependent loss of viability in SH-SY5Y cells, consistent with an earlier study 33 . However, in agreement with previous reports, different concentrations of Van negated the loss in cell viability induced by MPP + and improved cell survival in SH-SY5Y cells 34 .…”

Evaluation of Potential Neuroprotective Effects of Vanillin Against MPP+/MPTP-Induced Dysregulation of Dopaminergic Regulatory Mechanisms in SH-SY5Y Cells and a Mouse Model of Parkinson’s Disease

“…Here, we report the Van-mediated neuroprotection against MPP + -induced Parkinsonism in SH-SY5Y cells. In our study, administration of SH-SY5Y cells with MPP + resulted in a loss of viability in SH-SY5Y cells in concentration-dependent, consistent with an earlier study 34 . However, in agreement with previous reports, Van at different concentrations negated the MPP + induced loss in cell viability and improved cell survival in SH-SY5Y cells 35 .…”

Atractylenolide‐1 affects glycolysis/gluconeogenesis by downregulating the expression of TPI1 and GPI to inhibit the proliferation and invasion of human triple‐negative breast cancer cells

“…AT-1 was highly cytotoxic to human hepatoma cells and effectively inhibited the proliferation and migration of cancer cells. At the same time, AT-1 exerted a pro-apoptotic effect (More & Choi, 2017), consistent with the results of the current study.…”

“…AT‐1 was highly cytotoxic to human hepatoma cells and effectively inhibited the proliferation and migration of cancer cells. At the same time, AT‐1 exerted a pro‐apoptotic effect (More & Choi, 2017), consistent with the results of the current study. AT‐1 can promote apoptosis, inhibit the proliferation and migration of cancer cells, induce cell cycle arrest in cancer cells, and upregulate the expression of apoptosis‐related genes.…”