Atrazine binds to F1F0-ATP synthase and inhibits mitochondrial function in sperm

Hase, Yasuyoshi; Tatsuno, Michiko; Nishi, Takeyuki; Kataoka, Kosuke; Kabe, Yoshio; Yamaguchi, Yuki; Ozawa, Nobuaki; Natori, Michiya; Handa, Hiroshi; Watanabe, Hidenobu

doi:10.1016/j.bbrc.2007.11.107

Cited by 49 publications

(24 citation statements)

References 19 publications

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“…In Fig. 2A, mitochondria were isolated from rat liver, as described previously (Hase et al, 2008). The isolated mitochondria were incubated with 10 mM VK2 or 0.1 mg/ml FCCP for 3 hours and were then treated with 1 mg/ml JC-1 (Wako Pure Chemical) for 1 hour at 30°C.…”

Section: Methodsmentioning

confidence: 99%

Vitamin K2 Covalently Binds to Bak and Induces Bak-Mediated Apoptosis

et al. 2012

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Vitamin K2 (VK2, menaquinone) is known to have anticancer activity in vitro and in vivo. Although its effect is thought to be mediated, at least in part, by the induction of apoptosis, the underlying molecular mechanism remains elusive. Here, we identified Bcl-2 antagonist killer 1 (Bak) as a molecular target of VK2-induced apoptosis. VK2 directly interacts with Bak and induces mitochondrial-mediated apoptosis. Although Bak and Bcl-2-associated X protein (Bax), another member of the Bcl-2 family, are generally thought to be functionally redundant, only Bak is necessary and sufficient for VK2-induced cytochrome c (cyt c) release and cell death. Moreover, VK2-2,3 epoxide, an intracellular metabolite of VK2, was shown to covalently bind to the cysteine-166 residue of Bak. Several lines of evidence suggested that the covalent attachment of VK2 is critical for apoptosis induction. Thus this study reveals a specific role for Bak in mitochondria-mediated apoptosis. This study also provides insight into the anticancer effects of VK2 and suggests that Bak may be a potential target of cancer therapy.

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Section: Methodsmentioning

confidence: 99%

Vitamin K2 Covalently Binds to Bak and Induces Bak-Mediated Apoptosis

et al. 2012

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“…For instance, in the USA, almost 30,000 t of atrazine are applied annually (Hase et al 2008). Because of its intense use, stability and ability to translocate in the environment (Roustan et al 2014;Yixin et al 2014;Nachimuthu et al 2016), atrazine is present in soils and waters worldwide (Hase et al 2008;Merini et al 2009;Feria-Reyes et al 2011;Li et al 2012;Farlin et al 2013;Geng et al 2013;Ji et al 2015;Ouyang et al 2016a). Atrazine is suspected to be an endocrine disruptor and is linked to Parkinson's disease in humans (Filipov et al 2007;Brodeur et al 2009).…”

Section: Introductionmentioning

confidence: 99%

The interaction between atrazine and the mineral horizon of soil: a spectroscopic study

et al. 2017

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Purpose In this paper, an attempt was made to explain the long-lasting occurrence of atrazine in soil. Despite the fact that this herbicide has been banned in European Union 10 years ago, it is still detected in the environment. Materials and methods Soil samples (organic and mineral horizon), SiO 2 and Al 2 O 3 sorbents were spiked with atrazine. The ultrasound-assisted extraction coupled with gas chromatography-electron capture detector was performed to establish the atrazine recovery depending on the type of soil horizon and sorbent. Fourier transform infrared spectroscopy (FTIR) analysis was conducted to determine the type of interactions between atrazine and sorbents. Results and discussion The atrazine recovery was lower for the mineral horizon (15%) compared to the organic horizon (63%). This finding suggests an interaction between atrazine and the mineral components of soil. Therefore, attempts have been made to explain atrazine's interaction with the main mineral components of soil, SiO 2 and Al 2 O 3 , and to investigate the influence of pH on atrazine's behaviour in soil. The atrazine recoveries were 86.5 and 10.7% for Al 2 O 3 and SiO 2 , respectively. The obtained results demonstrated that the protonated atrazine exhibits stronger interactions with the soil mineral layer (recovery below 0.1%) in comparison to molecular form of atrazine (recovery 86%). FTIR results suggested interactions between atrazine and SiO 2 . FTIR analysis revealed that 1,3,5-azidine ring interacts with SiO 2 molecule. Conclusions In acidic soil, atrazine remediation is limited, especially if the soil contains minerals with high SiO 2 contents. This situation may cause the long-lasting persistence of atrazine in soil.

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“…ATZ is metabolized in mammalian organisms by Glutathione S-transferases and cytochrome P450 systems and affects the expression of Star, Cyp19a1 and many other genes (35,36). A series of experiments with sperm and isolated mitochondria suggest that ATZ inhibits mitochondrial function through its action on F1–F0-adenosine triphosphate (ATP) synthase, which is a major cellular protein capable of binding to ATZ (37). Recent study showed that ATZ also affects DNA methylation in plants (38).…”

Section: Introductionmentioning

confidence: 99%

Exposure to the widely used herbicide atrazine results in deregulation of global tissue-specific RNA transcription in the third generation and is associated with a global decrease of histone trimethylation in mice

et al. 2016

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The epigenetic events imposed during germline reprogramming and affected by harmful exposure can be inherited and transferred to subsequent generations via gametes inheritance. In this study, we examine the transgenerational effects promoted by widely used herbicide atrazine (ATZ). We exposed pregnant outbred CD1 female mice and the male progeny was crossed for three generations with untreated females. We demonstrate here that exposure to ATZ affects meiosis, spermiogenesis and reduces the spermatozoa number in the third generation (F3) male mice. We suggest that changes in testis cell types originate from modified transcriptional network in undifferentiated spermatogonia. Importantly, exposure to ATZ dramatically increases the number of transcripts with novel transcription initiation sites, spliced variants and alternative polyadenylation sites. We found the global decrease in H3K4me3 occupancy in the third generation males. The regions with altered H3K4me3 occupancy in F3 ATZ-derived males correspond to altered H3K4me3 occupancy of F1 generation and 74% of changed peaks in F3 generation are associated with enhancers. The regions with altered H3K4me3 occupancy are enriched in SP family and WT1 transcription factor binding sites. Our data suggest that the embryonic exposure to ATZ affects the development and the changes induced by ATZ are transferred up to three generations.

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Atrazine binds to F1F0-ATP synthase and inhibits mitochondrial function in sperm

Cited by 49 publications

References 19 publications

Vitamin K2 Covalently Binds to Bak and Induces Bak-Mediated Apoptosis

Vitamin K2 Covalently Binds to Bak and Induces Bak-Mediated Apoptosis

The interaction between atrazine and the mineral horizon of soil: a spectroscopic study

Exposure to the widely used herbicide atrazine results in deregulation of global tissue-specific RNA transcription in the third generation and is associated with a global decrease of histone trimethylation in mice

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