Atrial endothelial cells senescence promotes thrombogenicity, inflammation and extracellular matrix remodeling: Role of the local Ang II/AT1 receptor pathway

Hasan, Hira; Abbas, Malak; Auger, Cyril; Belcastro, Eugenia; Farooq, Muhammad; Park, S.H.; Ohlmann, Patrick; Toti, Florence; Schini‐Kerth, Valérie B.; Morel, Olivier; Jesel-Morel, Laurence

doi:10.1016/j.acvdsp.2018.02.102

Cited by 2 publications

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“…For example, secretion of atherosclerosis-related factors, such as intercellular adhesion molecule-1 and the inhibitory activity of endothelial nitric oxide synthase, is found in senescent human umbilical vein endothelial cells (HUVECs) (2). Atrial endothelial senescence was found to promote thrombus formation and extracellular matrix remodeling (3). In an aging mouse model with vascular endothelium damage, antioxidant therapy can alleviated the dysfunction of aging vascular endothelium cells (4).…”

Section: Introductionmentioning

confidence: 99%

Piwi-interacting RNAs play a role in vitamin C-mediated effects on endothelial aging

Zheng

Huang

et al. 2020

Int. J. Med. Sci.

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The underlying mechanisms that mediate the effects of vitamin C on endothelial cell aging are widely unknown. To investigate whether Piwi-interacting RNAs (piRNAs) are involved in this process, an endothelial aging model was induced in vitro using H2O2 in human umbilical vein endothelial cells (HUVECs) and then treated with vitamin C (VC). Untreated HUVECs without H2O2 exposure were used to serve as the negative control group. Cell cycle, cell viability, and aging-associated protein expression were assessed, and RNA sequencing was performed to reveal the piRNA profile. Functional and regulatory networks of the different piRNA target genes were predicted by the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment and Gene Ontology (GO) analysis. H2O2 induced G1 phase cell arrest, decreased cell viability, and upregulated the senescence marker p16 in HUVECs. We found that VC treatment inhibited G1 phase cell arrest, increased the number of cells in the S and G2/M phases, increased cell viability, and decreased p16 expression. The piRNA expression profiles revealed that a large proportion of piRNAs that were differentially expressed in H2O2-treated HUVECs were partly normalized by VC. Furthermore, a number of piRNAs associated with the response to VC in H2O2-treated HUVECs were linked with senescence and cell cycle-related pathways and networks. These results indicate that the ability of VC to attenuate H2O2-mediated endothelial cell senescence may be associated with changes in expression of piRNAs that are linked to the cell cycle.

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Section: Introductionmentioning

confidence: 99%

Piwi-interacting RNAs play a role in vitamin C-mediated effects on endothelial aging

Zheng

Huang

et al. 2020

Int. J. Med. Sci.

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“…Angiotensin II directly stimulates NADPH oxidase activity, which further results in the redox-dependent activation of ROS production by other cellular sources [71]. EC senescence has been shown to stimulate AT 1 receptor activity [72], and treatment with AT 1 receptor blockers has protective effects against endothelial senescence [73].…”

Section: Hop and Its Major Compounds Xn And IX Downregulate Redox-sen...mentioning

confidence: 99%

Prevention of Fine Dust-Induced Vascular Senescence by Humulus lupulus Extract and Its Major Bioactive Compounds

et al. 2020

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Both short- and long-term exposure to fine dust (FD) from air pollution has been linked to various cardiovascular diseases (CVDs). Endothelial cell (EC) senescence is an important risk factor for CVDs, and recent evidence suggests that FD-induced premature EC senescence increases oxidative stress levels. Hop plant (Humulus lupulus) is a very rich source of polyphenols known to have nutritional and therapeutic properties, including antioxidant behavior. The aims of this study were to evaluate whether Humulus lupulus extract prevents FD-induced vascular senescence and dysfunction and, if so, to characterize the underlying mechanisms and active components. Porcine coronary arteries and endothelial cells were treated with FD in the presence or absence of hop extract (HOP), and the senescence-associated-beta galactosidase (SA-β-gal) activity, cell-cycle progression, expression of senescence markers, oxidative stress level, and vascular function were evaluated. Results indicated that HOP inhibited FD-induced SA-β-gal activity, cell-cycle arrest, and oxidative stress, suggesting that HOP prevents premature induction of senescence by FD. HOP also ameliorated FD-induced vascular dysfunction. Additionally, xanthohumol (XN) and isoxanthohumol (IX) were found to produce the protective effects of HOP. Treatment with HOP and its primary active components XN and IX downregulated the expression of p22phox, p53, and angiotensin type 1 receptor, which all are known FD-induced redox-sensitive EC senescence inducers. Taken together, HOP and its active components protect against FD-induced endothelial senescence most likely via antioxidant activity and may be a potential therapeutic agent for preventing and/or treating air-pollution-associated CVDs.

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Atrial endothelial cells senescence promotes thrombogenicity, inflammation and extracellular matrix remodeling: Role of the local Ang II/AT1 receptor pathway

Cited by 2 publications

References 0 publications

Piwi-interacting RNAs play a role in vitamin C-mediated effects on endothelial aging

Piwi-interacting RNAs play a role in vitamin C-mediated effects on endothelial aging

Prevention of Fine Dust-Induced Vascular Senescence by Humulus lupulus Extract and Its Major Bioactive Compounds

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