Atrial natriuretic factor binding sites in rat area postrema: autoradiographic study

Konrad, Edyta M.; Thibault, Gaétan; Schiffrin, E. L.

doi:10.1152/ajpregu.1992.263.4.r747

Cited by 4 publications

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“…In particular, the area postrema has natriuretic peptide binding sites with pharmacological characteristics of the NP A and NP B but not the NP C receptor subtypes. 25 Commonalities in central connections of cardiopulmonary mechanosensitive and chemosensitive afferent pathways make this an interesting possibility, although there is no evidence yet either for or against it.…”

Section: Discussionmentioning

confidence: 99%

Guanylyl Cyclase Receptors Mediate Cardiopulmonary Vagal Reflex Actions of ANP

Thomas

Woods

2003

Hypertension

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Abstract-Atrial natriuretic peptide (ANP) potentiates vagal cardiopulmonary reflexes due to chemosensory (BezoldJarisch [B-J] reflex) or mechanosensory (ramp baroreflex) activation. The ANP receptor mediating these actions is unknown. We examined the role of particulate guanylyl-cyclase (pGC) receptors in ANP-induced enhancement of cardiopulmonary vagal reflexes. Cardiopulmonary baroreceptor reflex function was assessed by bradycardic responses to ramp blood pressure rises after rapid intravenous methoxamine (100 g/kg bolus dose). The B-J reflex was evoked by 3 intravenous doses of serotonin (1 to 10 g/kg). In conscious, chronically instrumented rats (nϭ9), these tests were performed on each animal during randomized infusions of rat ANP (150 ng/kg per minute IV), saline (270 L/h IV), the pGC receptor antagonist HS-142-1 (3 mg/kg IV), or combined HS-142-1ϩANP treatment. 4,5 There are 3 known natriuretic peptide receptor subtypes: A (NP A ), B (NP B ), and C (NP C ). 8 Most of the well-known biological actions, such as vasodilation and natriuresis, of the natriuretic peptides occur subsequent to stimulating the production of the second-messenger cyclic guanosine 3Ј 5Ј-monophosphate (cGMP), through particulate guanylylcyclase (pGC)-coupled receptors (the NP A and NP B receptors). The NP C receptor fulfills the role of a clearance receptor for the 3 natriuretic peptides. 9 In addition, while not having guanylylcyclase activity, this receptor has been reported to influence 3 signal-transducing systems: phospholipase C, adenylyl cyclase, and calcium channels. 9 The NP C receptor is proposed to be a physiological regulator of activities such as adrenergic transmission, renin and progesterone secretion, and platelet aggregation. 9 Although there is a growing body of literature linking natriuretic peptide receptors to the more widely known cardiovascular and renal actions of ANP, BNP, and CNP, 10 -15 the nature of the natriuretic peptide receptors involved in the HR reflex activities of these hormones has not been studied or reported in the literature. Since BNP and CNP share with ANP the ability to enhance reflex bradycardic responses, 4,5 a common receptor may mediate these effects. In the present studies, the contribution from the guanylyl cyclase receptors to the cardiopulmonary vagal baroreflex and chemoreflex (Bezold-Jarisch [B-J]) actions of ANP was investigated. HS-142-1 was used to selectively antagonize the NP A ϩNP B guanylyl cyclase-coupled (pGC) receptors. 16 HS-142-1 is a polysaccharide microbial product isolated from the culture broth of Aureobasidium species. 16 HS-142-1 blocks the vasodilator and renal effects of exogenously administered ANP and BNP and also reverses the changes resulting from augmented endogenous production of ANP and BNP. 10,17,18 Although HS-142-1 can exclude a role for the non-guanylyl cyclase receptor NP C , 16 this compound does not distinguish between subclasses of the pGC natriuretic peptide receptors.

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Section: Discussionmentioning

confidence: 99%

Guanylyl Cyclase Receptors Mediate Cardiopulmonary Vagal Reflex Actions of ANP

Thomas

Woods

2003

Hypertension

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“…The literature confirms that several of the identified receptors are expressed in the AP, such as the angiotensin II type 1a receptor ( Agtr1a ; Lenkei et al 1998; Huang et al 2003), vasopressin 1a receptor ( V1aR ; Tribollet et al 1999; Yang et al 2006), atrial natriuretic peptide receptor a and b ( Npr1/2 ; Konrad et al 1992 a , b ), prolactin receptor ( Prlr ; Mangurian et al 1999), neuropeptide Y1 ( Nyp1r ) and Y5 ( Nyp5r ) receptors (Dumont et al 1996; Dumont et al 1998 a , b ), glucagon‐like peptide‐1 receptor ( Glpr ; Price et al 2008), cannabinoid 1 receptor ( Cnr1 ; Partosoedarso et al 2003), adiponectin receptor ( AdipoR1/R2 ; Fry et al 2006) and receptor activity modifying protein (RAMP) mRNAs 1, 2 and 3 (Ueda et al 2001). While these RAMPs are not strictly receptors, they are accessory proteins that confer receptor specificity; for example, all three RAMPs heterodimerize with the calcitonin receptor to form three different receptors for amylin, a hormone co‐secreted with insulin (Christopoulos et al 1999; Muff et al 1999).…”

Section: Discussionmentioning

confidence: 85%

The transcriptome of the medullary area postrema: the thirsty rat, the hungry rat and the hypertensive rat

Hindmarch

Fry

Smith

et al. 2011

Experimental Physiology

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The area postrema (AP) is a sensory circumventricular organ characterized by extensive fenestrated vasculature and neurons which are capable of detecting circulating signals of osmotic, cardiovascular, immune and metabolic status. The AP can communicate these messages via efferent projections to brainstem and hypothalamic structures that are able to orchestrate an appropriate response. We have used microarrays to profile the transcriptome of the AP in the Sprague-Dawley (SD) and Wistar-Kyoto rat and present here a comprehensive catalogue of gene expression, focusing specifically on the population of ion channels, receptors and G proteincoupled receptors expressed in this sensory tissue; of the G protein-coupled receptors expressed in the rat AP, we identified ∼36% that are orphans, having no established ligand. We have also looked at the ways in which the AP transcriptome responds to the physiological stressors of 72 h dehydration (DSD) and 48 h fasting (FSD) and have performed microarrays in these conditions. Comparison between the DSD and SD or between FSD and SD revealed only a modest number of AP genes that are regulated by these homeostatic challenges. The expression levels of a much larger number of genes are altered in the spontaneously hypertensive rat AP compared with the normotensive Wistar-Kyoto control rat, however. Finally, analysis of these 'hypertensionrelated' elements revealed genes that are involved in the regulation of both blood pressure and immune function and as such are excellent targets for further study.

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Area postrema is essential for the maintenance of normal blood pressure under cold stress in rats

Qian¹,

Koon²

1998

Experimental Brain Research

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No final conclusion has yet been achieved on whether the area postrema (AP) is involved in the regulation of cardiovascular activity in the rats. The aim of the present study was to investigate the role of the AP in the regulation of basal blood pressure under normal as well as abnormal (cold stress) conditions in Sprague-Dawley rats. The lesion of AP was performed by the electrolytic-lesion method. Stressed animals were subjected to chronic intermittent cold stress (2 degrees C, 3 h/day for 14 days). The systolic blood pressure was measured by the indirect tail-cuff transducer method. The results showed that no significant difference was found between systolic blood pressure measured before and after AP-lesion surgery. The AP-lesion group had similar systolic blood pressure to both sham-operation and the control groups under normal environmental conditions. However, it was found that cold stress resulted in a significant increase in systolic blood pressure in the AP-lesion rats, but not in sham-lesion animals, within two weeks. Furthermore, there was no significant difference between blood pressures of sham-lesion rats with or without cold stress and the control animals. These results support the view that the AP plays no role in keeping basal blood pressure under normal condition and indicate as well that the AP is important in maintaining normal blood pressure under the conditions of stress (cold).

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Atrial natriuretic factor binding sites in rat area postrema: autoradiographic study

Cited by 4 publications

References 0 publications

Guanylyl Cyclase Receptors Mediate Cardiopulmonary Vagal Reflex Actions of ANP

Guanylyl Cyclase Receptors Mediate Cardiopulmonary Vagal Reflex Actions of ANP

The transcriptome of the medullary area postrema: the thirsty rat, the hungry rat and the hypertensive rat

Area postrema is essential for the maintenance of normal blood pressure under cold stress in rats

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