Atrial natriuretic peptide, brain natriuretic peptide and c‐type natriuretic peptide: effects on testicular microcirculation and immunohistochemical localization

Collin, Ola; Lissbrant, Erik; Bergh, Anders

doi:10.1046/j.1365-2605.1997.00108.x

Cited by 20 publications

(15 citation statements)

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“…1C are consistent with this conclusion because positive CNP staining was found in the interstitium and immunoreactive CNP was detected in Leydig cells, macrophages, and/or endothelial cells ( Fig. 1 Cb and Cf ), similar to the findings of two earlier reports (20,21). The steady-state mRNA level of the CNP receptor NPR-B, however, was very low in germ cells vs. testes or Sertoli cells (Fig.…”

Section: Expression Of Cnp In the Seminiferous Epithelium Of Adult Rasupporting

confidence: 90%

C-type natriuretic peptide regulates blood–testis barrier dynamics in adult rat testes

Xia

Mruk

Cheng

2007

Proc. Natl. Acad. Sci. U.S.A.

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In adult rat testes, the blood-testis barrier (BTB) in the seminiferous epithelium must ''open'' (or ''disassemble'') to accommodate the migration of preleptotene spermatocytes from the basal to the adluminal compartment that occurs at stage VIII of the epithelial cycle. However, the molecule(s) and/or mechanism(s) that regulate this event are unknown. In this report, C-type natriuretic peptide (CNP) was shown to be a regulator of BTB dynamics. Although Sertoli and germ cells contributed to the pool of CNP in the seminiferous epithelium, its receptor, natriuretic peptide receptor B, resided almost exclusively in Sertoli cells. CNP also expressed stage-specifically and localized predominantly at the BTB in the seminiferous epithelium at stage VIII of the epithelial cycle. A synthetic CNP-22 peptide, when added to Sertoli cell cultures, was shown to perturb Sertoli cell tight junction in vitro, causing disappearance of BTB-associated proteins (JAM-A, occludin, N-cadherin, and ␤-catenin) from the cell-cell interface. This inhibitory effect of CNP on the tight junction was confirmed by transient overexpression of CNP in these cells, which was mediated, at least in part, by accelerating the internalization of BTB integral membrane proteins. To validate these in vitro findings, CNP-22 was administered to testes at a dose of 0.35 or 3.5 g per testis, which was shown to perturb the BTB integrity in vivo when the barrier function was assessed by monitoring the diffusion of a small molecular probe across the BTB. In summary, CNP secreted by Sertoli and germ cells into the BTB microenvironment regulates BTB dynamics during spermatogenesis.anchoring junction ͉ spermatogenesis ͉ tight junction ͉ ectoplasmic specialization ͉ endocytosis

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Section: Expression Of Cnp In the Seminiferous Epithelium Of Adult Rasupporting

confidence: 90%

C-type natriuretic peptide regulates blood–testis barrier dynamics in adult rat testes

Xia

Mruk

Cheng

2007

Proc. Natl. Acad. Sci. U.S.A.

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“…However, this possibility exists, as certain putative target genes for GATA-4, such as brain-type natriuretic factor, are expressed in both testicular somatic cells and cardiomyocytes (41,42). Whether these GATA-binding proteins regulate a common group of target genes in these various tissues in unknown.…”

Section: Discussionmentioning

confidence: 99%

Expression and Regulation of Transcription Factors GATA-4 and GATA-6 in Developing Mouse Testis¹

et al. 1999

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Previous studies have shown that transcription factors GATA-4 and GATA-6 are expressed in granulosa and thecal cells of the mouse ovary and that GATA-4 expression in ovarian tissue is regulated by gonadotropins. Given the emerging role of GATA-4 and GATA-6 in gonadal cells, we have now studied the expression and regulation of these factors in the mouse testis and testicular cell lines. In situ hybridization demonstrated GATA-4 messenger RNA (mRNA) in the fetal testis at 13.5 days postcoitum. Both GATA-4 and GATA-6 transcripts were observed in late fetal, neonatal, juvenile, and adult Sertoli cells. In addition, GATA-4 mRNA was detected in interstitial cells throughout development. Immunohistochemistry demonstrated GATA-4 protein in both Sertoli and Leydig cells in postnatal animals. The regulation of GATA-4 and GATA-6 expression was explored using established testicular cell lines. Treatment of Leydig tumor cell lines with hCG resulted in a modest, but statistically significant, increase in the steady state level of GATA-4 mRNA, comparable to the previously described effect of FSH on GATA-4 expression in Sertoli cell lines. Gonadotropin or androgen action was not, however, a prerequisite for the basal expression of GATA-4 or GATA-6 in the testis, as their presence in Sertoli and Leydig cells was demonstrated in genetically hypogonadal hpg mice, in rats treated with GnRH receptor antagonist, and in Sertoli cells after chemical abolition of Leydig cells. Cotransfection studies using a GATA-4 expression plasmid and an inhibin alpha promoter/reporter gene construct in Leydig and granulosa tumor cell lines revealed that the inhibin alpha promoter harboring essential GATA-binding sites can be trans-activated by GATA-4. In light of these results, we propose that transcription factors GATA-4 and GATA-6 play differing roles in the maturation and function of testicular somatic cells.

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“…Furthermore, researchers have reported that NPPA was expressed in foetal, progenitor and immature Leydig cell populations with a varied immunoreactive density (Mourdjeva et al, ). Collin, Lissbrant, and Bergh () also reported that NPPB is localised to Leydig cells in rats.…”

Section: Introductionmentioning

confidence: 90%

Spatiotemporal expression patterns of natriuretic peptides in rat testis and epididymis during postnatal development

et al. 2019

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Natriuretic peptide (NP) family is composed of atrial, brain and C-type NP (NPPA, NPPB and NPPC). Here, we aimed to investigate NP expression in testis and epididymis during postnatal development. NPPA expression was observed in gonocytes at prepubertal period but in only spermatocytes in pachytene and leptotene/ zygotene stage at pubertal period. In prepubertal and pubertal periods, we detected NPPB expression in only Leydig cells. However, NPPC expression was detected in all of the gonocytes and Sertoli cells, spermatocytes and some interstitial cells in prepubertal and pubertal periods. In postpubertal and mature periods, NPPA and NPPB staining were detected in Leydig cells, elongated and round spermatids but not in spermatogonia and spermatocytes. However, we observed NPPC expression in all cells of the seminiferous tubules and Leydig cells in the postpubertal and mature periods. Epididymal epithelium showed intense NPPC expression during postnatal period but weak NPPA and NPPB expression in prepubertal and pubertal periods.The expression of three NPs in the testis significantly increased after puberty. In conclusion, puberty had a significant effect on NP expression in testis. Unlike NPPA and NPPB, expression of NPPC in all cells of the seminiferous tubule suggests that NPPC is effective in each step of spermatogenesis. K E Y W O R D Satrial natriuretic peptide, brain natriuretic peptide, C-type natriuretic peptide, epididymis, rat, testis

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Atrial natriuretic peptide, brain natriuretic peptide and c‐type natriuretic peptide: effects on testicular microcirculation and immunohistochemical localization

Cited by 20 publications

References 20 publications

C-type natriuretic peptide regulates blood–testis barrier dynamics in adult rat testes

C-type natriuretic peptide regulates blood–testis barrier dynamics in adult rat testes

Expression and Regulation of Transcription Factors GATA-4 and GATA-6 in Developing Mouse Testis¹

Spatiotemporal expression patterns of natriuretic peptides in rat testis and epididymis during postnatal development

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Atrial natriuretic peptide, brain natriuretic peptide and c‐type natriuretic peptide: effects on testicular microcirculation and immunohistochemical localization

Cited by 20 publications

References 20 publications

C-type natriuretic peptide regulates blood–testis barrier dynamics in adult rat testes

C-type natriuretic peptide regulates blood–testis barrier dynamics in adult rat testes

Expression and Regulation of Transcription Factors GATA-4 and GATA-6 in Developing Mouse Testis1

Spatiotemporal expression patterns of natriuretic peptides in rat testis and epididymis during postnatal development

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Expression and Regulation of Transcription Factors GATA-4 and GATA-6 in Developing Mouse Testis¹