Attachment and Detachment of Living Microorganisms Using a Potential-Controlled Electrode

Koyama, Sumihiro; Konishi, Masaaki; Ohta, Yutaka; Miwa, Tetsuya; Hatada, Yuji; Toyofuku, Takashi; Maruyama, Tadashi; Nogi, Yuichi; Kato, Chiaki; Tsubouchi, Taishi

doi:10.1007/s10126-013-9495-2

Cited by 14 publications

(52 citation statements)

References 36 publications

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“…The electrode-absorbed micro-organisms were released into artificial seawater (Nihon Pharmaceutical) by applying a triangular wave potential of ±10 mV, 9 MHz (Koyama et al, 2013). A portion of the retrieved suspension was then incubated in artificial seawater containing 0.5 % (w/v) ISP2 (MB; Difco) at 25 u C for 1 week for restoration of bacteria following electrical injury.…”

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Brevundimonas denitrificans sp. nov., a denitrifying bacterium isolated from deep subseafloor sediment

Tsubouchi

Koyama

Mori

et al. 2014

International Journal of Systematic and Evolutionary Microbiology

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A novel Gram-stain-negative, aerobic, heterotrophic, stalked and capsulated bacterium with potential denitrification ability, designated strain TAR-002 T , was isolated from deep seafloor sediment in Japan. Colonies lacked lustre, and were viscous and translucent white. The ranges of temperature, pH and salt concentration for growth were 8-30 6C, pH 6.0-10.0 and 1-3 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences confirmed that strain TAR-002 T belongs to the genus Brevundimonas of the class Alphaproteobacteria. Levels of similarity between the 16S rRNA gene sequence of strain TAR-002 T and those of the type strains of species of the genus Brevundimonas were 93.5-98.9 %; the most closely related species was Brevundimonas basaltis. In DNA-DNA hybridization assays between strain TAR-002 T and its phylogenetic neighbours, Brevundimonas lenta DS-18 T , B. basaltis J22 T , Brevundimonas subvibrioides ATCC 15264 T and Brevundimonas alba DSM 4736 T , mean hybridization levels were 6.4-27.7 %. The G+C content of strain TAR-002 T was 70.3 mol%. Q-10 was the major respiratory isoprenoid quinone. The major fatty acids were C 18 : 1 v7c and C 16 : 0 , and the presence of 1,indicates the affiliation of strain TAR-002 T with the genus Brevundimonas. On the basis of biological characteristics and 16S rRNA gene sequence comparisons, strain TAR-002 T is considered to represent a novel species of the genus Brevundimonas, for which the name Brevundimonas denitrificans sp. nov. is proposed; the type strain is TAR-002 T (5NBRC 110107 T 5CECT 8537 T ).

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Brevundimonas denitrificans sp. nov., a denitrifying bacterium isolated from deep subseafloor sediment

Tsubouchi

Koyama

Mori

et al. 2014

International Journal of Systematic and Evolutionary Microbiology

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“…Those isolation methods include a variety of physicochemical pretreatments such as phenol, calcium carbonate, salinity, antimicrobial treatment, heat, ultrasonic treatment, microwave and radio wave irradiation, and ionizing radiation. 3,24,30 In our previous study, we found that numerous actinomycetes including the genus Streptomyces could be electrically retrieved from deep-sea sediments 31 and cryopreserved marine sponges. 32 Living microorganisms suspended in nonnutritive solution such as PBS(¹) and artificial seawater (ASW) were selectively attached to a negative potential-applied optically transparent electrode against gravitational force.…”

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“…31, 32 Microorganisms suspended in Luria-Bertani medium and glucose solution were not attracted to the electrode. 31 Dead microorganisms were also not attracted to the electrode. 31 We collected the electrically attached living microorganisms and then detached them from the electrode by applying a high-frequency wave potential in nonnutritive solution such as PBS(¹) or ASW.…”

Section: Introductionmentioning

confidence: 99%

“…31 Dead microorganisms were also not attracted to the electrode. 31 We collected the electrically attached living microorganisms and then detached them from the electrode by applying a high-frequency wave potential in nonnutritive solution such as PBS(¹) or ASW. [31][32][33] The mechanisms by which the high-frequency wave potential induced electrical detachment involve both oscillation of the negative zeta potential-charged cells and insertion of water molecules between the electrode surface and attached cells.…”

Section: Introductionmentioning

confidence: 99%

“…31 We collected the electrically attached living microorganisms and then detached them from the electrode by applying a high-frequency wave potential in nonnutritive solution such as PBS(¹) or ASW. [31][32][33] The mechanisms by which the high-frequency wave potential induced electrical detachment involve both oscillation of the negative zeta potential-charged cells and insertion of water molecules between the electrode surface and attached cells. [31][32][33][34] High-frequency wave potential-induced cell detachment is practically noncytotoxic, [31][32][33][34] since 91% of the residual soil microorganisms attached to the ITO electrode remained alive after 60 min of a «10 mV vs. Ag/AgCl, 9 MHz triangular-wave potential application in PBS(¹).…”

Section: Introductionmentioning

confidence: 99%

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Electrical Retrieval of Living Streptomycete Spores Using a Potential-Controlled ITO Electrode

et al. 2017

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The purpose of this study was to develop a novel electrical retrieval method for living spores of streptomycetes. Five strains of deep-sea Streptomyces sp. and typical strain Streptomyces albus suspended in 1/20 artificial seawater (1/20ASW) were selectively attached to an indium tin oxide/glass (ITO) working electrode region to which a −0.2 V vs. Ag/AgCl constant potential was applied for 24 h. Spores of all six streptomycetes produced either short fibrous or membranous materials and attached to the ITO electrode region. A +20 mV vs. Ag/AgCl, 12 MHz sine-wave potential was further applied for another 1 h to the deep-sea Streptomyces sp. ST.28 spores on the ITO electrode and colony-forming units increased 3.5 fold after 3 day cultivation compared with controls. The ST.28 spores almost completely lost the ability to adhere to the potential-applied ITO electrode when dispersed in Mg 2+ free 1/20ASW. We succeeded in the attachment and cultivation of specifically positioned single ST.28 spores on an ITO microelectrode surface with −0.2 V vs. Ag/AgCl potential application. A combination of micropatterning techniques for a living single streptomycete spore on the ITO electrode and omics technologies holds potential for new bioactive compound screening concepts and applications.

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Double‐Emulsion Globules as a Tool to Produce 2D Patterned Metal Deposits Using Electro‐Colloidal Lithography

Faure

Leal-Calderón

Saadaoui

2014

Adv Materials Inter

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micro-particles used to stabilize the emulsion translate to either the poles or the equator of the drops, depending on the relative dielectric constants of the particles, on the surrounding fl uid and on the fl uid within the drop. Such motions break the particle barrier, thus allowing the drops to merge and therefore the emulsion to be destabilized. In a different approach, Dommersnes et al. [ 4 ] demonstrated that AC fi elds could also induce the organization of colloidal particles (metallic, clays or polymers) in "equatorial" ribbons and "longitudinal" dipolar chains at the surface of silicone drops dispersed in castor oil.Oil-in-water emulsions (O/W) under electric fi eld were disregarded until recently since it is diffi cult to destabilize them electrically because of the high conductivity of the continuous phase: the current densities required to induce signifi cant chain formation are much larger compared to those required for W/O emulsions. Vigo and Ristenpart [ 5 ] recently developed a lowvoltage technique for separating stable O/W emulsions. They were able to induce lateral aggregation and further coalescence of the oil droplets by applying a transversal AC electric fi eld, thus proving that the aggregation behavior is strongly reminiscent of that observed under similar electric fi eld conditions for rigid colloids. [ 6 ] In the same vein, here we provide evidence that double-emulsion globules (W/O/W) behave exactly like solid colloidal particles, and that they can self-assemble into planar close-packed or non-close packed hexagonal crystals. Moreover, we take advantage of this property to produce patterned micro-structures using a process we recently developed, called electro-colloidal lithography. [ 7 ] Like conventional colloidal lithography, electro-colloidal lithography relies on the fabrication of a colloidal mask. In colloidal lithography, surface patterning combines colloidal crystal formation with other techniques such as etching, imprinting or electrochemical deposition of a metal salt. [ 8 ] The added-value of electro-colloidal lithography lies in its simplicity since both the colloidal mask and the patterned surface are generated by the application of electric fi elds. The process begins with the arrangement of polystyrene micro-beads into close-packed hexagonal crystals (hpc) or in non-close packed hexagonal crystals (hnpc) using an AC electric fi eld. After fi xing 2D-microbeads crystals onto the electrode surface, a second population of colloidal particles is introduced; either these particles A technique developed to self assemble solid colloidal particles under a sinusoidal electric fi eld (AC fi eld) is adapted to soft W/O/W double-emulsion globules, and is exploited for surface patterning. Double-emulsions containing cupric ions are prepared, placed between two planar ITO electrodes and submitted to a transversal AC fi eld which induced their ordering into hexagonal 2D-arrays. The characteristic spacing is monitored by varying the globule volume fraction. Such self-assembly is used...

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Attachment and Detachment of Living Microorganisms Using a Potential-Controlled Electrode

Cited by 14 publications

References 36 publications

Brevundimonas denitrificans sp. nov., a denitrifying bacterium isolated from deep subseafloor sediment

Brevundimonas denitrificans sp. nov., a denitrifying bacterium isolated from deep subseafloor sediment

Electrical Retrieval of Living Streptomycete Spores Using a Potential-Controlled ITO Electrode

Double‐Emulsion Globules as a Tool to Produce 2D Patterned Metal Deposits Using Electro‐Colloidal Lithography

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