Attachment and extracellular matrix differences between tendon and synovial fibroblastic cells

Riederer-Henderson, Mary Ann; Gauger, Ann K.; Olson, Lynn; Robertson, Christie P.; Greenlee, Theodore K.

doi:10.1007/bf02621896

Cited by 43 publications

(16 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…5-8). The generation time data were consistent with the earlier report (24), in that SCs had a longer generation time than did IFs ( Fig. 4A and B, Table 1).…”

Section: Discussionsupporting

confidence: 92%

Cell populations of tendon: A simplified method for isolation of synovial cells and internal fibroblasts: Confirmation of origin and biologic properties

Banes

Donlon

Link

et al. 1988

Journal Orthopaedic Research

View full text Add to dashboard Cite

Tendons transmit the force of muscle contraction to bone to effect limb movement. Special structural and biological properties of tendon have developed to facilitate force transmission. The tendon has a complex organization of cells surrounding the collagen bundles inside tendon as well as at the tendon surface. Internal cells may act to maintain the bulk of the collagen in tendon. External cells in the epitenon may provide lubrication for tendon gliding. To develop better understanding of these processes and the roles the cell populations play, we isolated cells from the surface and interior of tendon and studied them in vitro. Flexor tendons from 8-week-old white Leghorn chickens were separated into two distinct cell populations: the outer synovial cells and the fibroblasts more internal in tendon. These cell populations were discernible by their locations in the intact tendon, determined by sequential enzymatic and physical release from their substrata. Initially, some cells eluted in Hanks' salt solution (HSS) (population 1); then synovial cells were released after a 2-min treatment with 0.5% collagenase (population 2). Next, a population of synovial cells was released in high yield by treatment with 0.25% trypsin (step III, population 3). Step III, population 3 cells were used as synovial cells (SCs). Next, a population of SCs and fibroblasts were released by scraping with a rubber policeman (population 4). Subsequently, fibroblasts were released after incubation with 0.5% collagenase (population 5). A more direct procedure (procedure 2) to isolate the synovial and internal tendon cells involved treatment in 0.5% collagenase followed by sedimentation at 900 g. Cells that sedimented were largely fibroblasts, whereas the cells that remained at the top of the tube were largely SCs. Cells designated as SCs, isolated by procedure 2, most likely contained surface cells from epitenon and internal interfascicular cells from endotenon and paratenon. Surface tendon cells separated by sequential enzymatic and physical release from their substrata (by procedure 1) had all the following characteristics: distinct subpopulations of cells based on morphology; presence of cytoplasmic, lipid-containing vesicles; decreased sensitivity to trypsin; and reduced generation time as compared with that of internal fibroblasts. Conversely, the internal fibroblasts (IFs) appeared to represent a more uniform population based on morphological characteristics.

show abstract

“…5-8). The generation time data were consistent with the earlier report (24), in that SCs had a longer generation time than did IFs ( Fig. 4A and B, Table 1).…”

Section: Discussionsupporting

confidence: 92%

Cell populations of tendon: A simplified method for isolation of synovial cells and internal fibroblasts: Confirmation of origin and biologic properties

Banes

Donlon

Link

et al. 1988

Journal Orthopaedic Research

View full text Add to dashboard Cite

show abstract

“…also be increased. But the level of collagen type III would be expected to be lower than that of collagen type I, since fibroblasts in culture predominately produce type I collagen (Riederer-Henderson et al, 1983). Future studies are required to investigate the dependency of collagen gene expression and collagen production on rest time.…”

Section: Discussionmentioning

confidence: 97%

Proliferation and collagen production of human patellar tendon fibroblasts in response to cyclic uniaxial stretching in serum-free conditions

Yang

Crawford

Wang

2004

Journal of Biomechanics

259

195

View full text Add to dashboard Cite

“…1). Internally, the endotenon is composed of stress-oriented collagen bundles infiltrated with fibroblasts (18,31,32). The synovial aspect, or epitenon, of the tendon lies between the tendon and extratendinous tissues and is composed of synovial cells in a matrix of collagen, proteoglycan, and lipid (36).…”

mentioning

confidence: 99%

“…Riederer-Henderson et al (31) and Banes et al (6) have presented evidence that the tendon synovial cells are morphologically and biochemically distinct from the internal fibroblasts. Qualitative differences noted in the latter study included increased expression of fibronectin (Fn) in synovial cells as compared with internal fibroblasts in the tendon.…”

mentioning

confidence: 99%

Fibronectin in the tendon‐synovial complex: Quantitation In vivo and In vitro by elisa and relative mRNA levels by polymerase chain reaction and northern blot

Brigman

Yin

et al. 1994

Journal Orthopaedic Research

View full text Add to dashboard Cite

An enzyme-linked immunosorbent assay was used to quantitate fibronectin (Fn) levels in the outer synovia (epitenon) and internal fibrous portion (endotenon) of chicken flexor tendon and sheath. Primary cell cultures from these tissues and their secretions also were assayed for Fn levels. The polymerase chain reaction (PCR) was used to determine relative steady-state levels of Fn mRNA in primary cultures of synovial and internal fibroblasts from chicken tendon, and Northern blot analysis was performed to verify relative levels of the Fn message. The epitenon contained 3.8-fold more Fn than did the endotenon, and the sheath synovium contained 21-fold more Fn than did the internal fibrous portion of sheath. Cells cultured from the epitenon produced 9.3 and 13-fold more cell-associated and secreted Fn, respectively, than did cultured endotenon fibroblasts. Sheath synovial cells produced 17 and 3.2-fold more cell-associated and secreted Fn, respectively, than did sheath internal fibroblasts. Levels of Fn mRNA, as measured by PCR and Northern blot, were 1.6 and 1.8-fold greater, respectively, in tendon synovial cells compared with tendon internal fibroblasts. The biologic reason for increased Fn in tendon synovium is not known. We theorize that Fn may stabilize tendon synovium to shear stress and may play a role in the modulation of synovial rheology in the normal tendon. In the injured tendon, Fn may be involved in the organization of collagen deposition or may act through association with growth factors to aid healing.

show abstract

Attachment and extracellular matrix differences between tendon and synovial fibroblastic cells

Cited by 43 publications

References 29 publications

Cell populations of tendon: A simplified method for isolation of synovial cells and internal fibroblasts: Confirmation of origin and biologic properties

Cell populations of tendon: A simplified method for isolation of synovial cells and internal fibroblasts: Confirmation of origin and biologic properties

Proliferation and collagen production of human patellar tendon fibroblasts in response to cyclic uniaxial stretching in serum-free conditions

Fibronectin in the tendon‐synovial complex: Quantitation In vivo and In vitro by elisa and relative mRNA levels by polymerase chain reaction and northern blot

Contact Info

Product

Resources

About