Attachment and Growth of Fibroblast Cells on Poly (2-Methoxyethyl Acrylate) Analog Polymers as Coating Materials

Abstract

The regulation of adhesion and the subsequent behavior of fibroblast cells on the surface of biomaterials is important for successful tissue regeneration and wound healing by implanted biomaterials. We have synthesized poly(ω-methoxyalkyl acrylate)s (PMCxAs; x indicates the number of methylene carbons between the ester and ethyl oxygen), with a carbon chain length of x = 2–6, to investigate the regulation of fibroblast cell behavior including adhesion, proliferation, migration, differentiation and collagen production. We found that PMC2A suppressed the cell spreading, protein adsorption, formation of focal adhesion, and differentiation of normal human dermal fibroblasts, while PMC4A surfaces enhanced them compared to other PMCxAs. Our findings suggest that fibroblast activities attached to the PMCxA substrates can be modified by changing the number of methylene carbons in the side chains of the polymers. These results indicate that PMCxAs could be useful coating materials for use in skin regeneration and wound dressing applications.

Improving hard metal implant and soft tissue integration by modulating the “inflammatory-fibrous complex” response

“…They can also secrete ECM and generate contraction that presses the soft tissue against the implant, forming another layer of metal soft tissue integration. Fibroblasts can also act as immunomodulators, turning metal soft tissue integration into the immune barrier against external stimuli [ [30] , [31] , [32] , [33] , [34] ]. Therefore, we speculate that macrophages and fibroblasts interact closely as one complex.…”

Modulation of Biological Responses of Tumor Cells Adhered to Poly(2-methoxyethyl acrylate) with Increasing Cell Viability under Serum-Free Conditions

“…Poly­(2-methoxyethyl acrylate) (PMEA), a water-insoluble polymer, is a biomaterial coating; , substrates coated with PMEA possess the properties of antithrombogenicity and cell adhesivity based on the modulation of protein adsorption and conformational changes in the presence of serum proteins. PMEA suppresses the adsorption and conformational changes of fibrinogen, which plays a key role in blood clot formation, while enhancing the conformational change in adsorbed fibronectin (FN), an adhesive protein containing the RGD sequence. − The profiles of adsorbed proteins on PMEA substrates inhibited the adhesion of platelets and enhanced the adhesion of cells, such as fibroblasts, hepatocytes, adipocytes, endothelial cells, and tumor cells. − Furthermore, we discovered that the number of adhered cells on PMEA substrates increased with the expression of integrin α5 and β3 . Because integrin expression in tumor cells is relatively higher than that in normal cells, PMEA substrates could be used to isolate tumor cells. , Currently, CTCs are mainly collected using specific antibody-conjugated substrates; however, PMEA has the potential to isolate tumor cells including CTCs from whole blood without using antibodies, thus allowing the label-free isolation of tumor cells.…”

Nanoscopic analyses of cell-adhesive protein adsorption on poly(2-methoxyethyl acrylate) surfaces

“…Our previous reports demonstrated that the amounts of fibrinogen and fibronectin adsorbed onto the PMEA substrates were consistent with their monolayer configurations, resulting in negligible changes in their heights. 14,31 In contrast, these substrates exhibited a decrease in the elastic modulus of the surface in a time-dependent manner. The histograms for the elastic moduli of the protein-adsorbed substrates were constructed from their moduli images (Fig.…”