Attachment of horseradish peroxidase (HRP) onto the poly(styrene/acrolein) latexes and onto their derivatives with amino groups on the surface; activity of immobilized enzyme

Unique Activity of Ureases Immobilized on Poly(Styrene-Co-Acrolein) Microspheres

“…For example, for the immobilization of human serum albumin (HSA), human gamma globulins (G) and horseradish peroxidase (HRP) we found that À HSA (max) was 1.14 mg/m 2 , À G (max) was 2.10 mg/m 2 and À HRP (max) was 1.24 mg/m 2 ( [17,23,26]). It should be noted, however, that immobilization of HRP, due to the absence of free -NH 2 groups in commercial preparation of this enzyme, was carried on using P(S/A) microspheres with aldehyde groups replaced with -NH 2 groups (in reaction with ethylene diamine) and HRP with sugar moieties oxidized to -CHO [23]. The above considerations indicate that for both types of ureases (Urs-JB and Urs-PP) the immobilization was similar to that for other proteins investigated.…”

“…Moreover, during prolonged storage proteins that are only adsorbed may slowly dissipate into the solution. Covalent immobilization of proteins ensures permanent binding, however, this process also leads to reduction of protein biological activity [20][21][22][23]. The best results were obtained with surfaces that prevented undesired protein adsorption but at the same time allowed covalent immobilization of the proteins under mild conditions in a simple manner.…”

Chemical degradation of poly(2-aminoethyl methacrylate)

“…Primary amine-based copolymers have been exploited for numerous applications, including drug carriers [1][2][3], functional colloidal particles for biomedical applications [4][5][6], the preparation of sterically-stabilized polyaniline particles [7] and cationic latexes [8], the synthesis of polypeptide vesicles and micelles [9], and the localized biomineralization of calcium carbonate and silica [10,11].…”

Characterization of surface NH+3Cl− groups on poly(styrene-co-Boc-aminostyrene) microspheres obtained by controlled acidic treatment

“…These particles can be diazotized or reacted with determined spacers (for example, glutaraldehyde), which can attach molecules of antigen, antibodies, or enzymes [1,2].…”