Attachment of Rod-Like (BAR) Proteins and Membrane Shape

Kabaso, Doron; Gongadze, Ekaterina; Elter, Patrick; Rienen, Ursula van; Gimsa, Jan; Kralj‐Iglič, Veronika; Iglič, Aleš

doi:10.2174/138955711795305353

Cited by 40 publications

(53 citation statements)

References 62 publications

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“…20 Third, changes in local membrane curvature followed by wrapping of NPs and their agglomerates may lead to lipid depletion from the bilayer, and thus altered membrane integrity and/ or formation of pores or holes. 16,22,[53][54][55][56][61][62][63][64] In the case of GUVs, we propose that the most likely mechanism causing GUVs to burst is adhesion of CAadsorbed CoFe 2 O 4 NPs and smaller NP agglomerates onto membranes, resulting in alteration of the global membrane curvature and, finally, bursting. The POPC lipids used in our study have an externally situated lipid polar head (the trimethylammonium group) that is positively charged and faces the aqueous glucose solution.…”

Section: Mlvs After Incubation In Np Suspensionsmentioning

confidence: 99%

Effects of magnetic cobalt ferrite nanoparticles on biological and artificial lipid membranes

Drašler¹,

Drobne²,

Novak³

et al. 2014

IJN

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Background The purpose of this work is to provide experimental evidence on the interactions of suspended nanoparticles with artificial or biological membranes and to assess the possibility of suspended nanoparticles interacting with the lipid component of biological membranes. Methods 1-Palmitoyl-2-oleoyl- sn -glycero-3-phosphocholine (POPC) lipid vesicles and human red blood cells were incubated in suspensions of magnetic bare cobalt ferrite (CoFe 2 O 4 ) or citric acid (CA)-adsorbed CoFe 2 O 4 nanoparticles dispersed in phosphate-buffered saline and glucose solution. The stability of POPC giant unilamellar vesicles after incubation in the tested nanoparticle suspensions was assessed by phase-contrast light microscopy and analyzed with computer-aided imaging. Structural changes in the POPC multilamellar vesicles were assessed by small angle X-ray scattering, and the shape transformation of red blood cells after incubation in tested suspensions of nanoparticles was observed using scanning electron microscopy and sedimentation, agglutination, and hemolysis assays. Results Artificial lipid membranes were disturbed more by CA-adsorbed CoFe 2 O 4 nanoparticle suspensions than by bare CoFe 2 O 4 nanoparticle suspensions. CA-adsorbed CoFe 2 O 4 -CA nanoparticles caused more significant shape transformation in red blood cells than bare CoFe 2 O 4 nanoparticles. Conclusion Consistent with their smaller sized agglomerates, CA-adsorbed CoFe 2 O 4 nanoparticles demonstrate more pronounced effects on artificial and biological membranes. Larger agglomerates of nanoparticles were confirmed to be reactive against lipid membranes and thus not acceptable for use with red blood cells. This finding is significant with respect to the efficient and safe application of nanoparticles as medicinal agents.

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Section: Mlvs After Incubation In Np Suspensionsmentioning

confidence: 99%

Effects of magnetic cobalt ferrite nanoparticles on biological and artificial lipid membranes

Drašler¹,

Drobne²,

Novak³

et al. 2014

IJN

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“…Recently, the anisotropic nature of curvature has received increasing attention theoretically. The classical Canham-Helfrich curvature free energy [32,33] has been extended to anisotropic curvatures [34][35][36]. To simplify the interactions, the protein and membrane underneath it have been often modeled together as an undeformable object with a fixed curved shape such as a point-like object with an anisotropic curvature [37,38] and a bent elliptical surface [39].…”

Section: Introductionmentioning

confidence: 99%

Shape deformation of lipid membranes by banana-shaped protein rods: Comparison with isotropic inclusions and membrane rupture

Noguchi

2016

Phys. Rev. E

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The assembly of curved protein rods on fluid membranes is studied using implicit-solvent meshless membrane simulations. As the rod curvature increases, the rods on a membrane tube assemble along the azimuthal direction first and subsequently along the longitudinal direction. Here, we show that both transition curvatures decrease with increasing rod stiffness. For comparison, curvature-inducing isotropic inclusions are also simulated. When the isotropic inclusions have the same bending rigidity as the other membrane regions, the inclusions are uniformly distributed on the membrane tubes and vesicles even for large spontaneous curvature of the inclusions. However, the isotropic inclusions with much larger bending rigidity induce shape deformation and are concentrated on the region of a preferred curvature. For high rod density, high rod stiffness, and/or low line tension of the membrane edge, the rod assembly induces vesicle rupture, resulting in the formation of a high-genus vesicle. A gradual change in the curvature suppresses this rupture. Hence, large stress, compared to the edge tension, induced by the rod assembly is the key factor determining rupture. For rod curvature with the opposite sign to the vesicle curvature, membrane rupture induces inversion of the membrane, leading to division into multiple vesicles as well as formation of a high-genus vesicle.

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“…However, another explanation consistent with the above findings is that the majority of nanoparticles are created after blood sampling, and accordingly the results reflect the properties and composition of blood cells and plasma. For example, membrane and plasma constituents may affect membrane curvature, [47][48][49] which is the basic mechanism underlying the budding and vesiculation of membranes. Controlled manipulation of the size of the cell fragments (nanoparticles) could be advantageous, as avoiding production of fragments of the size of immune complexes (mean diameter approximately 150 nm) may avoid problems with artefacts when measuring the nanoparticle concentration by flow cytometry.…”

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confidence: 99%

Nanoparticles isolated from blood: a reflection of vesiculability of blood cells during the isolation process

Šuštar¹,

Bedina-Zavec²,

Stukelj³

et al. 2011

IJN

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Background: Shedding of nanoparticles from the cell membrane is a common process in all cells. These nanoparticles are present in body fluids and can be harvested by isolation. To collect circulating nanoparticles from blood, a standard procedure consisting of repeated centrifugation and washing is applied to the blood samples. Nanoparticles can also be shed from blood cells during the isolation process, so it is unclear whether nanoparticles found in the isolated material are present in blood at sampling or if are they created from the blood cells during the isolation process. We addressed this question by determination of the morphology and identity of nanoparticles harvested from blood. Methods: The isolates were visualized by scanning electron microscopy, analyzed by flow cytometry, and nanoparticle shapes were determined theoretically. Results: The average size of nanoparticles was about 300 nm, and numerous residual blood cells were found in the isolates. The shapes of nanoparticles corresponded to the theoretical shapes obtained by minimization of the membrane free energy, indicating that these nanoparticles can be identified as vesicles. The concentration and size of nanoparticles in blood isolates was sensitive to the temperature during isolation. We demonstrated that at lower temperatures, the nanoparticle concentration was higher, while the nanoparticles were on average smaller. Conclusion:These results indicate that a large pool of nanoparticles is produced after blood sampling. The shapes of deformed blood cells found in the isolates indicate how fragmentation of blood cells may take place. The results show that the contents of isolates reflect the properties of blood cells and their interaction with the surrounding solution (rather than representing only nanoparticles present in blood at sampling) which differ in different diseases and may therefore present a relevant clinical parameter.

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Attachment of Rod-Like (BAR) Proteins and Membrane Shape

Cited by 40 publications

References 62 publications

Effects of magnetic cobalt ferrite nanoparticles on biological and artificial lipid membranes

Effects of magnetic cobalt ferrite nanoparticles on biological and artificial lipid membranes

Shape deformation of lipid membranes by banana-shaped protein rods: Comparison with isotropic inclusions and membrane rupture

Nanoparticles isolated from blood: a reflection of vesiculability of blood cells during the isolation process

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