Work-life conflict is an important problem, especially for women, because it can affect their well-being and career choices. This research important because work-life conflict is a complicated issue driven by a variety of factors. These issues are critical for developing supportive work environments that foster a good work-life balance, especially for women. This study investigates of Islamic variables on reduce work life conflict among Minangkabau women in diverse organizational and job settings. The research methodology used explanatory through seven hypothesis testing either direct and mediation effect. Data collected from 200 respondents through purposive sampling using a survey approach. The research instrument incorporated variables such as Islamic work ethic, gratitude, religiosity. Confirmatory Factor Analysis (CFA) and Structural Equation Modelling (SEM) were employed for data analysis. Results findings indicate a positive influence of Islamic work ethic and gratitude, positive influence of religiosity and gratitude and also negative influence of religiosity and work life conflict. However, hypotheses concerning Islamic work ethic and gratitude on work life conflict were not supported, thus it can be concluded that Islamic work ethic and gratitude in this case, it cannot lower the work life conflict on the Minangkabau working female in West Sumatera Indonesia. In the same way with the mediation relationship, the two hypothesis of mediation proved that gratitude is not as a mediator the relationship between Islamic work ethic and religiosity to work life conflict. Further research proposed that research on work-life conflict among women can explore various dimensions to provide a comprehensive understanding of this complex issue.