Attenuated Total Reflection  Fourier Transform Infrared (Atr-Ftir): A Method for the Biochemical Study of Walnut Leaves

Topala, Carmen Mihaela; Vîjan, Loredana Elena; Giura, Simona Ileana; Botu, M.

doi:10.47068/ctns.2020.v9i17.033

Cited by 3 publications

(4 citation statements)

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“…Background reference spectra were recorded using air after every sample to minimize the interference due to carbon dioxide and water vapor in the atmosphere. Between measurements, the ATR crystal was carefully cleaned using pure acetone (Sigma-Aldrich Co., Saint Louis, MO, USA), then, it was dried with soft tissue [43][44][45].…”

Section: Uv-vis and Atr-ftir Analysismentioning

confidence: 99%

“…Spectra were preprocessed using the second-derivative transformation, the Savitzky-Golay derivation. The use of spectra derivatives with the Savitzky-Golay algorithm as a chemometric pre-processing technique has been widely reported in most classifications that are based on FTIR spectroscopy [43][44][45]. Multivariate analysis (e.g., principal component analysis, PCA; hierarchical cluster analysis, HCA; linear discriminate analysis, LDA) was previously often used to evaluate and/or classify honey depending on its chemical composition, physicochemical, or biological properties [28,46].…”

Section: Chemometric Analysismentioning

confidence: 99%

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Botanical Origin Influence on Some Honey Physicochemical Characteristics and Antioxidant Properties

Vîjan,

Mazilu,

Enache

et al. 2023

Foods

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Five types of honey (multifloral, sunflower, linden, rapeseed, and acacia), from Southern Romania, were classified using chemometrics methods coupled with IR spectroscopy. The botanical origin’s effect on the physicochemical characteristics of honey was studied to highlight the most valuable plant source of honey. Except for antioxidant activity, the moisture, ash, electrical conductivity (EC), pH, free acidity (FA), total sugar content (TSC), hydroxymethylfurfural (HMF), total phenolic (TPC), tannin (TTC), and flavonoid content (TFC) were significantly influenced by the botanical origin of the honey. The results showed that sunflower honey had the highest moisture (15.53%), free acidity (16.67 mEq kg−1), electrical conductivity (483.92 µS cm−1), phenolics (167.59 mg GAE 100 g−1), and flavonoids (19.00 mg CE 100 g−1), whereas multifloral honey presented the highest total sugar content (69.64 g Glu 100 g−1). The highest HMF content was found in linden honey (33.94 mg kg−1). The HMF contents of all tested honey were within the standard recommended limit, and they confirmed that the tested honey was free of any heat treatment. All five types of tested honey presented a safe moisture content for storage and consumption (12.21–18.74%). The honey′s free acidity was in the range of 4.00 to 25.00 mEq kg−1; this indicated the freshness of the samples and the absence of any fermentation processes in the tested honey. Honey with a total sugar content over 60% (except for linden honey, with 58.05 g glucose 100 g−1) showed the characteristic of nectar-derived honey. The elevated antioxidant activity of honey was correlated with its high moisture, flavonoids, and HMF, whereas the tannins and HMF were positively correlated with ash and electrical conductivity. The higher content of phenolics, flavonoids, and tannins was correlated with higher free acidity. The chemometric method, coupled with ATR-FTIR spectra, revealed a clear separation between linden honey from acacia, multifloral, and sunflower honey.

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Section: Uv-vis and Atr-ftir Analysismentioning

confidence: 99%

Section: Chemometric Analysismentioning

confidence: 99%

Botanical Origin Influence on Some Honey Physicochemical Characteristics and Antioxidant Properties

Vîjan,

Mazilu,

Enache

et al. 2023

Foods

Self Cite

View full text Add to dashboard Cite

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Section: Methodsmentioning

confidence: 99%

Application of Spectroscopy in Analysis of Some Floral Honey Samples Collected From Four South Romanian Counties in 2021

Giosanu¹,

Topala²,

Vîjan³

et al. 2022

CTNS

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Honey is a concentrated aqueous solution of sugars, especially glucose and fructose, and minor amounts of dextrin, enzymes, volatile oils, organic acids, ethers, and minerals. Honey samples vary in quality according to various factors, such as climate diversity, geographical characteristics, floral supply period, and packaging and storage conditions, which can compromise the quality of the hive's final product. The assessment of honey quality is a major concern that has gained interest internationally because honey has always been subjected to various fraudulent practices which become more sophisticated in the present day. Consumers, beekeepers, and regulatory bodies are interested to have reliable analytical tools and information to allow the detection of adulterated honey. The UV-VIS spectroscopy and FTIR spectroscopy techniques are two analytical methods used to analyze the honey quality nowadays, with hydroxymethylfurfural being used as a standard for testing honey's freshness. The present study was conducted to assess the quality of some floral honey samples (rapeseed, acacia, and polyfloral honey) produced in Argeș, Călărași, Giurgiu, and Teleorman counties from the south of Romania. Differences between honey botanical and geographical origins were analyzed and highlighted with One-way ANOVA (p˂0.05), followed by Duncan`s Multiple Range Test. Our results indicated that honey is a valuable source of bioactive compounds.

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“…In addition to water (3-5%), walnuts contain proteins (12-25%), polyunsaturated fatty acids (omega 3 and omega 6), biogenic amines (melatonin and serotonin), vitamins (B, C, E, and P complex), minerals (magnesium, potassium, calcium, iron, zinc), polyphenols, and fiber [1,3,6,7]. Topala et al (2020) [8] cites authors that justified the classification of the walnut as a strategic nut crop for human nutrition as well as the inclusion in the dedicated FAO list of priority plants.…”

Section: Introductionmentioning

confidence: 99%

Effect of Temperature and Storage Time on Some Biochemical Compounds from the Kernel of Some Walnut Cultivars Grown in Romania

et al. 2023

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Walnut kernels are appreciated not only for their mineral content, vitamins, proteins, and valuable lipids, but also for the presence of polyphenolic compounds and biogenic amines. The temperature and storage time effects on some biochemical compounds from kernels collected from eight walnut genotypes grown at the Fruit Growing Research and Extension Station (SCDP) Valcea, Romania, were studied. In general, the phenolic and carotenoid compounds followed opposite trends during short-term storage and in some cases in medium-term storage. In both cases, there was a reduction in concentration during long-term storage, which was more pronounced for carotenoids. The most efficient method for preserving the walnut kernel’s phenolic content was freezing. Keeping the walnut kernel at room temperature assured the smallest carotenoid content decrease. Depending on the walnut cultivar, the preservation of the walnut kernel can be extended to five months by storage at −20 to −18 °C without loss of phenolic compounds, while a period shorter than five months (but certainly longer than three months) could be recommended for storage at 3–4 °C. Keeping it at room temperature can be an option for a short period (about three months). None of the preservation methods was adequate if the losses recorded for carotenoids were taken into account.

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Attenuated Total Reflection Fourier Transform Infrared (Atr-Ftir): A Method for the Biochemical Study of Walnut Leaves

Cited by 3 publications

References 0 publications

Botanical Origin Influence on Some Honey Physicochemical Characteristics and Antioxidant Properties

Botanical Origin Influence on Some Honey Physicochemical Characteristics and Antioxidant Properties

Application of Spectroscopy in Analysis of Some Floral Honey Samples Collected From Four South Romanian Counties in 2021

Effect of Temperature and Storage Time on Some Biochemical Compounds from the Kernel of Some Walnut Cultivars Grown in Romania

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