Attenuating Effect ofGinkgo bilobaLeaves Extract on Liver Fibrosis Induced by Thioacetamide in Mice

Abstract

The purpose of this study is to investigate the effect of Ginkgo biloba leaves extract on experimental liver fibrosis induced by thioacetamide (TAA) in male albino mice. The experimental mice were divided into four groups. The mice of the first group were served as control. The experimental animals of the second group were given 150 mg/kg body weight of TAA by intraperitoneal injection, twice weekly, for 9 weeks. The mice of the third group were exposed to TAA and supplemented with G. biloba leaves extract. The animals of the fourth group were supplemented with G. biloba leaves extract. The levels of plasma alanine aminotransferase, aspartate aminotransferase, gamma-glutamyl transferase, alkaline phosphatase, triglycerides, cholesterol, and low-density lipoprotein cholesterol were statistically increased while the levels of plasma total protein, albumin, glucose, and high-density lipoprotein cholesterol were significantly decreased. The levels of liver superoxide dismutase, glutathione, glycogen and total protein were notably declined, whereas the level of total lipid was increased in mice of the second group. Furthermore, microscopic examination of liver sections from mice treated with TAA showed an abnormal morphology characterized by nodular transformations in liver parenchyma which surrounded by fibrous septa. Administration of G. biloba leaves extract reduced extent and development of fibrous septa, liver cells change, and biochemical alterations in mice exposed to TAA. This study showed that G. biloba leaves extract has a potential activity against TAA-induced liver fibrosis and suggested that the chemical constituents of G. biloba are effective in modulation of oxidative stress induced by TAA.

Silymarin Protects Mouse Liver and Kidney from Thioacetamide Induced Toxicity by Scavenging Reactive Oxygen Species and Activating PI3K-Akt Pathway

“…In many cases TAA has been used to create a model of hepato-toxicity. These agents include reynosin (Lim et al, 2013), Ginkgo biloba leaves extract (Al-Attar, 2012) and ethanolic and aqueous extracts of Phyllanthus acidus (L.) Skeels ( P. Acidus ; Jain and Singhai, 2011), etc. An imidazolium salt, 1,3-diisopropylimidazolium tetrafluoroborate (DPIM) has also been shown to reduce TAA-induced mouse liver injury (Ding and Zhuo, 2013).…”

Protective Effect of <i>Gingo biloba</i> Extract on Carbendazim-Induced Hepatotoxicity in Albino Rats

“…Liu et al 2006 [39] demonstrated that the histopathological score of fibrosis and liver function were significantly improved in rats treated with ccl4 plus G. biloba extract, compared with those treated with ccl4 only. Zhang et al 2008 [40] reported that G. biloba extract EGb 761 alleviates hepatic fibrosis and sinusoidal microcirculation disturbance in patients with chronic hepatitis B. Al-Attar, 2012 [21] showed that G. biloba leaves extract has a potential activity against thioacetamide-induced liver fibrosis and suggested that the chemical constituents of G. biloba are effective in modulation of oxidative stress induced by thioacetamide.…”

“…Sener et al 2005 [20] assessed the antioxidant and antifibrotic effects of long-term G.biloba extract administration on liver fibrosis induced by bile duct ligation (BDL) and scission in Wistar male albino rats. Recently, Al-Attar, 2012 [21] reported that G. biloba leaves extract inhibits liver fibrosis induced by thioacetamide in mice. The present study was designed to evaluate the protective effect of G. biloba leaves extract on hepatotoxicity of carbendazim in albino rats.…”

Protective Effects of Omega Supplement on Induced Hepatic Mal-Function by Thioacetamide in Male Rats

“…To measure bilirubin, azo DVD reagents (sulfanilic acid sodium nitrite) were used; they react with bilirubin, producing azo, which is red in alkaline PH. After production, direct bilirubin has a pink color, but after addition of accelerating solution, total bilirubin turns green in alkaline PH [17].…”