Attenuating effect of Indole-3-Carbinol on gold nanoparticle induced hepatotoxicity in rats

Alkhalaf, Maha I.

doi:10.1016/j.arabjc.2020.09.035

Cited by 8 publications

(6 citation statements)

References 49 publications

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“…Many cyclic peptides used in clinic, and the most of them originate from the natural cyclic peptides [1]. As several features make cyclic peptides attractive lead compounds for drug development as well as nice tools for biochemical research, scientists made diverse efforts to develop biologically active cyclic peptide compounds [2][3][4][5][6]. Thus, synthetic peptides, as initial biological leads, allow rapid identification of the molecular structural requirements of active drug modulators.…”

Section: Introductionmentioning

confidence: 99%

Synthesis, spectroscopic, DFT and docking studies, molecular structure of new Schiff base metal complexes

Zayed

2021

Egypt. J. Chem.

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A Schiff base ligand (H2L) and its complexes have been synthesized and characterized by analytical tools, spectral (IR and 1 H NMR), molar conductivity and magnetic moment measurements. The thermal analysis (TG) technique is studied within the temperature range from room temperature to 1000 0 C. The IR spectra pointed out that the ligand (H2L) coordinated to the metal ions through two nitrogen and four oxygen atoms in a neutral mode. The structural formula of the synthesized Schiff base ligand was optimized using Gaussian09 program where the energy gaps and other important theoretical parameters were calculated applying the DFT/B3LYP method. In vitro biological activities of the Schiff base ligand (H2L) and its metal complexes were screened against Gram(+) bacteria (Bacillis subtilis and Staphylococcus aureus) and Gram(-) bacteria (Escherichia coli and Pseudomonas aeruginosa) using agar diffusion method. The results illustrated that the synthesized complexes are biologically active than the Schiff base ligand. Molecular docking study was carried out between the Schiff base ligand and crystal structure of Escherichia Coli, crystal structure of Staphylococcus Aureus, crystal structure of Bacillus subtilis and crystal structure of P. aeruginosa.

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Section: Introductionmentioning

confidence: 99%

Synthesis, spectroscopic, DFT and docking studies, molecular structure of new Schiff base metal complexes

Zayed

2021

Egypt. J. Chem.

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“…A recent study found that molecular docking of the I3C crystal structure had anticancer activity against hepatocellular carcinoma (Alkhalaf, 2020). Similarly, our docking score demonstrates that the I3C has a high affinity for 3DYC, which plays an important role in apoptosis in AGS cells by releasing cytochrome‐c from the mitochondria.…”

Section: Discussionmentioning

confidence: 99%

Indole‐3‐carbinol induces apoptosis in AGS cancer cells via mitochondrial pathway

Singh

Kiddane

et al. 2023

Chem Biol Drug Des

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Indole‐3‐carbinol is produced from the cruciferous vegetables and broadly investigated for their various biological effects in in‐vitro and in‐vivo aspects. However, the anticancer activity of I3C and its molecular mechanisms have not been investigated in human adeno gastro carcinoma (AGS) cells. In our study of AGS cells, nuclear condensation was observed by 4′,6‐diamidino‐2‐phenylindole (DAPI) staining, cell death was confirmed by a cell viability assay, and fragmented DNA was observed at the IC50 dose by a DNA fragmentation assay. Apoptosis was evaluated by the qPCR technique. Treatment of the AGS cells with I3C at different concentrations has drastically decreased cell proliferation and differentiation. By releasing cytochrome‐c from mitochondria in the intrinsic pathway, I3C prevents the multiplication of AGS cells and initiates apoptosis. The WST‐1 assay result showed that I3C treatment against AGS cells had considerably reduced the viability of the cells. Furthermore, RT‐qPCR showed the fold change among the expressed proteins compared with reference gene β‐actin. Molecular docking revealed that I3C showed a strong binding affinity for the apoptotic protein 3DCY. The results show the caspase group of proteins contribute to the core of apoptotic machinery. I3C and its metabolites target a variety of components of cell‐cycle control via distinct signaling pathways in light of the rapid development of tumors and oncogenesis. The translational significance of I3C and its metabolites in cancer is highlighted by their wide range of antitumor activity and low toxicity. Furthermore, the novel prodrug I3C, which has overlapping underlying mechanisms, could encourage new strategies to decrease oncogenesis.

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“…Additionally, I3C enhanced the antioxidant activity in anoxic pups through SOD, CAT, and GSH. Previous reports suggest that I3C activates the nuclear erythroid factor 2 (NRF2), and increasing the antioxidant enzyme levels (Alkhalaf, 2020) could be the reason for the antioxidant activity of I3C. Anoxia opens MPTP, allowing cytochrome C release from injured mitochondria (Samaiya et al, 2017), causing subsequent activation of caspases (9 and 3), resulting in cell death (McIlwain et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Indole‐3‐carbinol ameliorated the neurodevelopmental deficits in neonatal anoxic injury in rats

Kakarla

Krishnamurthy

2022

Intl J of Devlp Neuroscience

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Neonatal anoxia is linked to long‐lasting neurodevelopmental deficits. Due to the lack of pharmacological intervention to treat neonatal anoxia, there is interest in finding new molecules for its treatment. Indole‐3‐carbinol (I3C) has shown neuroprotective effects in some disease conditions. However, the neuroprotective role of I3C in neonatal anoxia has not been explored. Consequently, we have investigated the effect of I3C on neonatal anoxia‐induced brain injury and neurodevelopmental deficits. Rat pups after 30 h of birth were subjected to two episodes of anoxia (10 min in each) at a time interval of 24 h by flowing 100% nitrogen. I3C was administered within 30 min of the second episode of anoxia on a postnatal day (PND) 3 and continued for PND 9. Neurodevelopmental deficits, cortical mitochondrial membrane potential (MMP), opening of mitochondrial permeability transition pore (MPTP), electron transport chain (ETC) enzyme activities, oxidative stress, hypoxia‐inducible factor‐1α (HIF‐1α) levels, histopathological changes, and apoptosis were measured. I3C treatment dose‐dependently ameliorated the neurodevelopmental deficits and somatic growth in anoxic pups. I3C improved mitochondrial function by enhancing the MMP, mitochondrial ETC enzymes, and antioxidants. It blocked the MPTP opening and release of cytochrome C in anoxic pups. Further, I3C reduced the elevated cortical HIF‐1α in neonatal anoxic pups. Furthermore, I3C ameliorated histopathological abnormalities and mitochondrial‐mediated apoptotic indicators Cyt C, caspase‐9, and caspase‐3. Our study concludes that I3C improved neuronal development in anoxic pups by enhancing mitochondrial function, reducing HIF‐1α, and mitigating apoptosis.

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Attenuating effect of Indole-3-Carbinol on gold nanoparticle induced hepatotoxicity in rats

Cited by 8 publications

References 49 publications

Synthesis, spectroscopic, DFT and docking studies, molecular structure of new Schiff base metal complexes

Synthesis, spectroscopic, DFT and docking studies, molecular structure of new Schiff base metal complexes

Indole‐3‐carbinol induces apoptosis in AGS cancer cells via mitochondrial pathway

Indole‐3‐carbinol ameliorated the neurodevelopmental deficits in neonatal anoxic injury in rats

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