Attenuating potential of aluminum sulphate (alum) on testosterone propionate-induced benign prostatic hyperplasia in male Wistar rats

Abstract

The effect of aluminum sulphate (alum) on Testosterone propionate (TP)-induced Benign Prostatic Hyperplasia (BPH) in male Wistar rats was studied. Eighty mature male Wistar rats, with an average weight of 210g, were randomly distributed into eight groups comprising ten rats. Group 1 received only food and water, while Groups 2, and 4 to 8 were given 3 mg/kg b.w of TP subcutaneously and Group 3 received only 25 % alum solution for 28 days. Thereafter, Groups 4 to 8 were treated with 10%, 20%, 25%, 30%, and 40% alum solution respectively while group 2 remained untreated for another 28 days. The animals were fed with standard rat chow and clean water ad libitum. Sperm morphology and characteristics were observed and measured. A variety of haematological and biochemical markers were assessed. Histopathology of the testes was examined. The volume (0.10 ± 0.00 ml) viability (68.33 ± 4.41 %), activity (41.25 ± 1.25 %), and sperm count (36.67 ± 3.33 ×106) were significantly decreased (p<0.05) in group 2 when compared with the respective values obtained in group 1. Treatment with different concentrations of Alum solution significantly (p<0.05) reversed abnormal sperm features observed in group 2. Superoxide dismutase (0.90 ± 0.01 U/L) was significantly increased (p<0.05) in group 2, but the values were restored to normal after treatment with varying concentrations of Alum solutions. Photomicrographs of the testis of group 2 rats revealed a distorted testis, however, there was a significant recovery after treatment which suggested that treatment with Alum reduces TP-induced BPH.

The impact of Annona muricata and metformin on semen quality and hormonal profile in Arsenic trioxide-induced testicular dysfunction in male Wistar rat

“…Furthermore, ELAM significantly increased the FSH levels in the ameliorative and protective phase, which has its physiology linked to combating ROS formation attached to the flavonoids and saponins. Onyegeme-Okerenta et al(126) and Olowofolahan et al(127) reported a significant decline in FSH level following Annona muricata in reproductive dysfunction, which disagree with the study outcome. The study corroborates the findings of Abd El-Monem and Elwakeel(128) and El-Sawi et al(129), revealing a significantly higher level of FSH following Annona muricata extract against testicular toxicity.…”

Annona muricata Leaf Extract Modulates Chloramphenicol-Induced Lymphoma in Female Wistar Rats

“…The dried extract was weighed and stored in a clean reagent bottle, then preserved in a refrigerator at 4 O C before use. 22 Experimental design Forty-eight adult female Wistar rats with an average weight of 186 g were bought from animal breeders and kept in the Department of Biochemistry, University of Port Harcourt Animal house. The rats were allowed to get acclimatized to the environment for one week and had unrestricted access to normal rat chow and water ad libitum.…”

“…Numerous health benefits 20 have been attributed to A. muricata, most of which has been linked to its antioxidant potential. 21,22 This study was carried out to ascertain the effect of prolonged dose of chloramphenicol on the erythropoietic processes and its cytotoxic potential in inducing leukaemia and the modulatory prospective of aqueous leaf extract of A. muricata on CIL in female Wistar rats.…”