Attenuation of Acute Rat Renal Allograft Rejection by Apolipoprotein E-Mimetic Peptide

Zakrzewicz, Anna; Kutlimuratov, Khusin; Atanasova, Srebrena; Zakrzewicz, Dariusz; Reichert, Martin; Schmitz, Jessica; Wilhelm, Jochen; Lochnit, Günter; Padberg, Winfried

doi:10.1097/tp.0000000000000569

Transplantation

2015

DOI: 10.1097/tp.0000000000000569

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Attenuation of Acute Rat Renal Allograft Rejection by Apolipoprotein E-Mimetic Peptide

Anna Zakrzewicz

Khusin Kutlimuratov

Srebrena Atanasova

et al.

Abstract: Endogenous ApoE overexpression and exogenous application of ApoE(133-149) seem to protect renal allografts from fatal acute rejection. This effect was associated with a reduced influx of cytotoxic T cells.

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Quantification of mRNA Expression by RT-qPCR

Hutchinson¹

2015

Transplantation

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The real-time reverse transcription polymerase chain reaction (RT-qPCR) addresses the evident requirement for quantitative data analysis in molecular medicine, biotechnology, microbiology and diagnostics and has become the method of choice for the quantification of mRNA. Although it is often described as a ''gold'' standard, it is far from being a standard assay. The significant problems caused by variability of RNA templates, assay designs and protocols, as well as inappropriate data normalization and inconsistent data analysis, are widely known but also widely disregarded. As a first step towards standardization, we describe a series of RT-qPCR protocols that illustrate the essential technical steps required to generate quantitative data that are reliable and reproducible. We would like to emphasize, however, that RT-qPCR data constitute only a snapshot of information regarding the quantity of a given transcript in a cell or tissue. Any assessment of the biological consequences of variable mRNA levels must include additional information regarding regulatory RNAs, protein levels and protein activity. The entire protocol described here, encompassing all stages from initial assay design to reliable qPCR data analysis, requires approximately 15 h.

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Quantification of mRNA Expression by RT-qPCR

Hutchinson¹

2015

Transplantation

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Attenuation of Acute Rat Renal Allograft Rejection by Apolipoprotein E-Mimetic Peptide

Abstract: Endogenous ApoE overexpression and exogenous application of ApoE(133-149) seem to protect renal allografts from fatal acute rejection. This effect was associated with a reduced influx of cytotoxic T cells.

Cited by 1 publication

References 56 publications

Quantification of mRNA Expression by RT-qPCR

Quantification of mRNA Expression by RT-qPCR

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