Attenuation of Airway Obliteration by Ciprofloxacin in Experimental Posttransplant Bronchiolitis Obliterans

Remund, Kaspar; Rechsteiner, Thomas; Rentsch, Katharina; Vogt, Peter R.; Russi, Erich W.; Boehler, Annette

doi:10.1097/tp.0b013e31816632ed

Cited by 6 publications

(5 citation statements)

References 24 publications

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“…Remund K et al has shown that ciprofloxacin attenuates airway obliteration after tracheal transplantation and that this effect may be based on the modulation of the TH1/TH2-balance by this antibiotic drug. 20 As already described imbalance in the TH1/TH2 parameters play a role in the development of IBD. This may be another mechanism by which ciprofloxacin has shown antiinflammatory activity in the present study.…”

Section: Figure 1 (C): Histopathological Evaluationmentioning

confidence: 89%

Comparative evaluation of different doses of ciprofloxacin alone and in combination with sulfasalazine in experimentally induced inflammatory bowel disease in rats

Parashuram

Krishna

Lahkar

2016

Int J Basic Clin Pharmacol

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Inflammatory bowel disease (IBD) is an immunemediated chronic intestinal condition. Ulcerative colitis (UC) and Crohn's disease (CD) are the two major types of IBD. The incidence of IBD, especially UC, is rising in Japan, South Korea, Singapore, northern India and Latin America, areas previously thought to have low incidence. 1 Patients with CD and UC alternate between periods of active disease, which may require hospital admission, and periods of remission. The cause of IBD seems to include genetic, environmental, and immunologic components. Evidence suggests that IBD is triggered by an aberrant immune response to enteric flora, leading to intestinal inflammation. 2,3 Pathophysiological changes in IBD are well established, among which cytokines like tumor necrosis factor α (TNF-α), interleukin-1 (IL-1) and interleukin-8 (IL-8) are secreted from macrophages. 4 TNF-α upregulates the adhesion molecules (E selectin and ICAM-1) causing the adherence of neutrophils in endothelium and passage into ABSTRACT Background: Inflammatory bowel disease (IBD) is an idiopathic, chronic inflammatory condition, which affects the gastrointestinal tract and has no curative treatment. The present study aimed to investigate the effect of different doses of ciprofloxacin alone and in combination with sulfasalazine in AA (acetic acid)-induced inflammatory bowel disease (IBD) in rats. Methods: A total of 36 animals were included in the study. Animals were divided into five groups (n = 6): group I-control (normal saline), group II-AA+ normal saline, group III-sulfasalazine(360mg/kg) +AA, group IV Aciprofloxacin (50 mg/kg), group IV B-ciprofloxacin 100 mg/kg + AA, group V-ciprofloxacin 100 mg/kg + sulfasalazine (360 mg/kg) +AA. Group IV was divided into two subgroups, namely IVA and IVB, on the basis of different doses of ciprofloxacin used. After completion of two weeks of treatment, rats were sacrificed under ether anaesthesia for assessment of intestinal inflammation using parameters namely colon weight change, macroscopic and histopathological evaluation. Results: There was a decrease in colonic weight, macroscopic scores and microscopic scores in groups treated with ciprofloxacin at a dose of 100 mg/kg i.e. high dose given alone and in combination with sulfasalazine. Combination treatment was more effective when compared to single drug treatment. Conclusions: The present study indicates the efficacy of ciprofloxacin in acetic acid-induced IBD. The effects are more pronounced at higher dose i.e., 100 mg/kg. Combination of ciprofloxacin and sulfasalazine has shown greater efficacy than single drug treatment.

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Section: Figure 1 (C): Histopathological Evaluationmentioning

confidence: 89%

Comparative evaluation of different doses of ciprofloxacin alone and in combination with sulfasalazine in experimentally induced inflammatory bowel disease in rats

Parashuram

Krishna

Lahkar

2016

Int J Basic Clin Pharmacol

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“…Heterotopic tracheal transplantation was performed as previously described [14,15]. In short, donor tracheas were explanted, cut into halves, and implanted subcutaneously into the neck of recipient animals.…”

Section: Heterotopic Tracheal Transplantation and Experimental Groupsmentioning

confidence: 99%

“…Histologic slides were analyzed in a blinded fashion by computerized morphometry using Adobe Photoshop CS3 software (Adobe, San Jose, USA). The relative extent of tracheal obliteration, epithelial coverage and exposure of the basal membrane was calculated as described before [15].…”

Section: Histologymentioning

confidence: 99%

“…Total RNA was isolated from tracheal allografts as previously described [15]. RNA quality was assessed with a BioAnalyzer instrument and RNA-NanoChips (Agilent).…”

Section: Clarithromycin Affects Mediator Expression Early and Late Inmentioning

confidence: 99%

“…(A) The relative luminal obliteration of tracheal allografts early (5 days) and late (21 days) after transplantation was measured in a blinded fashion as previously described[15]. The white bars (0 mg/kg clarithromycin), the grey bars(10 mg/kg clarithromycin), and the black bars (30 mg/kg clarithromycin) show the percentage of luminal tracheal obliteration.…”

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confidence: 99%

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The Macrolide Clarithromycin Inhibits Experimental Post-Transplant Bronchiolitis Obliterans

Remund

Rechsteiner

Guo

et al. 2009

Experimental Lung Research

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Chronic allograft dysfunction in form of bronchiolitis obliterans is the most important hurdle to improved longterm survival after clinical lung transplantation to date. Recently, it was observed that the progression of bronchiolitis obliterans in lung transplant recipients might be inhibited by macrolide antibiotics. The authors therefore tested whether macrolide therapy can attenuate fibrous obliteration of airways in an animal model of bronchiolitis obliterans. Rats with heterotopic tracheal allografts were treated intraperitoneally with clarithromycin and compared to untreated transplanted animals with respect to allograft histology and expression of selected cytokines. At day 21 after transplantation, the tracheal allografts of treated animals were free of fibrous material or partially occluded dependent of clarithromycin dosage. Untreated animals had completely obliterated allografts. In treated animals, tumor necrosis factor alpha (TNF-alpha) was down-regulated early (5 days) and late (21 days) post transplant, whereas interferon gamma (IFN-gamma) expression was decreased only early after transplantation. Transforming growth factor beta (TGF-beta) expression was not affected. Therapy with low-dose macrolides in post-transplant obliterative bronchiolitis is based on their immunomodulatory rather than antimicrobial properties. In the setting of lung transplantation, macrolides primarily act as modulators of the early inflammatory response to stressed, damaged, or infected cells.

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A Review of Immunomodulatory Effects of Fluoroquinolones

Assar

Nosratabadi

Azad

et al. 2020

Immunological Investigations

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Past researches indicate that some types of antibiotics, apart from their antimicrobial effects, have some other important effects which indirectly are exerted by modulating and regulating the immune system's mediators. Among the compounds with antimicrobial effects, fluoroquinolones (FQs) are known as synthetic antibiotics, which exhibit the property of decomposing of DNA and prevent bacterial growth by inactivating the enzymes involved in DNA twisting, including topoisomerase II (DNA gyrase) and IV. Interestingly, immune responses are indirectly modulated by FQs through suppressing pro-inflammatory cytokines, such as interleukin 1 (IL-1), IL-6, tumor necrosis factor-alpha (TNF-α), and super-inducing IL-2, which tend to increase both the growth and activity of T and B lymphocytes. In addition, they affect the development of immune responses by influencing of expression of other cytokines and mediators. This study aims to review past research on the immunomodulatory effects of FQs on the expression of cytokines, especially IL-2 and to discuss controversial investigations.

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Attenuation of Airway Obliteration by Ciprofloxacin in Experimental Posttransplant Bronchiolitis Obliterans

Cited by 6 publications

References 24 publications

Comparative evaluation of different doses of ciprofloxacin alone and in combination with sulfasalazine in experimentally induced inflammatory bowel disease in rats

Comparative evaluation of different doses of ciprofloxacin alone and in combination with sulfasalazine in experimentally induced inflammatory bowel disease in rats

The Macrolide Clarithromycin Inhibits Experimental Post-Transplant Bronchiolitis Obliterans

A Review of Immunomodulatory Effects of Fluoroquinolones

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