Attenuation of Drought Stress in Brassica Seedlings with Exogenous Application of Ca2+ and H2O2

Abstract

Drought is one of the most common abiotic stresses, affecting the growth and productivity of crop plants globally, particularly in arid and semi-arid regions. Different strategies are used to mitigate the impact of drought among crop plants. Exogenous application of different substances are known to decrease the effects of various abiotic stresses, including drought stress. The aim of this study was to evaluate the effect of Ca2+ and H2O2 in developing drought stress tolerance in Brassica napus “Bulbul-98” seedlings. Brassica napus “Bulbul-98” seedlings were exposed to 5, 10 and 15 mM Ca2+ and 2, 5 and 10 μM H2O2 concentrations twice at an interval of two days for up to 20 days after germination. Drought stress decreased relative water content (RWC), chlorophyll content and increased proline, H2O2, soluble protein and electrolyte leakage in Brassica seedlings. Exogenous Ca2+ (5, 10,15 mM) and H2O2 (2, 5, 10 μM) supplementations, during drought stress induction, showed a significant increase in RWC by 5.4%, 18.06%, 26.2% and 6.87%, 13.9%, 18.3% respectively. Similarly, with the exogenous application of Ca2+ (5, 10, 15 mM) and H2O2 (2, 5, 10 μM), chlorophyll content was increased by 15.03%, 22.2%, and 28.4%, and 9.6%, 23.3%, and 27.5% respectively. It was confirmed that the seedlings under drought stress that were supplemented with Ca2+ and H2O2 recovered from water content reduction and chlorosis, and were able to grow normally.

Insights into 28-homobrassinolide (HBR)-mediated redox homeostasis, AsA–GSH cycle, and methylglyoxal detoxification in soybean under drought-induced oxidative stress

“…The H 2 O 2 increase in G. max during drought stress was consistent with a study on the response of Triticum aestivum to drought stress (Elkeilsh et al 2019). EL (%) was markedly increased in G. max under drought stress, which has been widely reported in crops (Assaha et al 2016;Khan et al 2017). In previous studies, changes in root activity were not reported with HBR application under drought stress.…”

“…Drought negatively influences plant growth and yield, especially in drought-prone areas of the world (Hasan et al 2020). The prolongation of drought may lead to critical physiological and developmental changes in plants (Khan et al 2017;Hasan et al 2019). Drought causes various metabolic and physiological abnormalities in plants such as loss of cell turgor, cell shrinkage, photosynthetic reduction, and imbalance in mineral content (Choudhury et al 2017;Dubey et al 2018;Yang et al 2019).…”

Hydrogen Sulfide-Mediated Activation of O-Acetylserine (Thiol) Lyase and l/d-Cysteine Desulfhydrase Enhance Dehydration Tolerance in Eruca sativa Mill

“…H 2 S acts as an important signaling molecule during plant response to biotic and abiotic stresses [21,22]. Moreover, H 2 S also acts as an antioxidant, and improves the performance of plants under stress conditions [23,24,25]. H 2 S plays a significant role in various physiological and biochemical processes, including seed germination, morphogenesis, photosynthesis, and flowering [26,27,28].…”

The Effect of Magnetized Water on the Growth and Physiological Conditions of Moringa Species under Drought Stress

“…production, considered as a major abiotic stress that has affected plant growth rate and development [3][4][5]. Due to changing climatic conditions, water shortages have become more apparent not just in arid regions, but humid regions as well [6].…”