Attenuation of lung inflammation and fibrosis in CD69-deficient mice after intratracheal bleomycin

Abstract

BackgroundCluster of differentiation 69 (CD69), an early activation marker antigen on T and B cells, is also expressed on activated macrophages and neutrophils, suggesting that CD69 may play a role in inflammatory diseases. To determine the effect of CD69 deficiency on bleomycin(BLM)-induced lung injury, we evaluated the inflammatory response following intratracheal BLM administration and the subsequent fibrotic changes in wild type (WT) and CD69-deficient (CD69-/-) mice.MethodsThe mice received a single dose of 3 mg/kg body weight of BLM and were sacrificed at 7 or 14 days post-instillation (dpi). Lung inflammation in the acute phase (7 dpi) was investigated by differential cell counts and cytokine array analyses of bronchoalveolar lavage fluid. In addition, lung fibrotic changes were evaluated at 14 dpi by histopathology and collagen assays. We also used reverse transcription polymerase chain reaction to measure the mRNA expression level of transforming growth factor β1 (TGF-β1) in the lungs of BLM-treated mice.ResultsCD69-/- mice exhibited less lung damage than WT mice, as shown by reductions in the following indices: (1) loss of body weight, (2) wet/dry ratio of lung, (3) cytokine levels in BALF, (4) histological evidence of lung injury, (5) lung collagen deposition, and (6) TGF-β1 mRNA expression in the lung.ConclusionThe present study clearly demonstrates that CD69 plays an important role in the progression of lung injury induced by BLM.

Role of peripheral dopaminergic system in the pathogenesis of experimental colitis in rats

“…, an early activation marker antigen on T and B cells, is also expressed on activated macrophages and neutrophils. It was found that CD69-deficient mice developed less bleomycin-induced lung injury and inflammation vs. wild type mice [36]. In our study, the number of CD69 positive granulocytes (Fig.…”

Prenatal inflammation exacerbates hyperoxia-induced functional and structural changes in adult mice

“…2A) and the quantification of Picrosirius red staining (Fig. 2B) as well as protein levels indicate expression of collagen I and III in the alveolar walls is much like those observed in infants with BPD and in animal models of pulmonary fibrosis (31,41,59). Consequently, we speculated that the increases in collagen observed in our model are more likely a deficiency in matrix remodeling rather than a dysregulation in deposition.…”

CD40 amplifies Fas-mediated apoptosis: a mechanism contributing to emphysema

“…Bronchoalveolar lavage (BAL) of mice was performed as describe elsewhere (40). In brief, the trachea was exposed and lavaged three times with 1 ml of PBS.…”