Attenuation of oxidative stress and hypertension in an animal model of HELLP syndrome

Morris, Rachael; Spencer, Shauna‐Kay; Barnes, A C; Bowles, Teylor; Kyle, Patrick B.; Wallace, Kedra

doi:10.1016/j.ejphar.2018.07.013

Cited by 7 publications

(8 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The alteration of ROS is associated with HELLP syndrome pathogenesis (Matsubara et al 2015, Morris et al 2018. The upregulation of ROS can cause tissue damage and the dysfunction of ET activation plays a critical role in hypertension in a rat model of HELLP syndrome (Matsubara et al 2015, Morris et al 2016.…”

Section: Discussionmentioning

confidence: 99%

“…The upregulation of ROS can cause tissue damage and the dysfunction of ET activation plays a critical role in hypertension in a rat model of HELLP syndrome (Matsubara et al 2015, Morris et al 2016. The blockade of the ET receptor can result in a decrease of ROS level and alleviation of the progression of HELLP syndrome in a rat model (Morris et al 2018). The level of SOD increased during normal pregnancy while the SOD activity is decreased in the patients with HELLP syndrome (Jauniaux et al 2000, Wang & Walsh 2001.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Inhibiting PDGF-D alleviates the symptoms of HELLP by suppressing NF-κB activation

Wang¹,

et al. 2021

Journal of Molecular Endocrinology

View full text Add to dashboard Cite

Hemolysis, elevated liver enzymes, and low platelet count (HELLP) syndrome is a life-threatening pregnancy complication. Though there are several medications widely used to treat HELLP syndrome, delivery is the only efficient treatment. The goal of the present study was to investigate the effects of platelet-derived growth factor-D (PDGF-D), a newly identified PDGF, in a rat model of HELLP syndrome which was accomplished by sFlt-1 and sEng injection. The expression levels of PDGF-D in pregnant women diagnosed with HELLP syndrome was determined. A HELLP rat model was established and the PDGF-D expression level in the plasma and the placenta tissue was evaluated. To evaluate the effects of PDGF-D in HELLP syndrome model, siPDGF-D was injected into the rats and the HELLP syndrome-related parameters were measured. The levels of inflammatory cytokines and PDGF-D were determined by enzyme-linked immunosorbent assay. The oxidative stress activities in the plasma were also determined. Furthermore, the expression of PDGF-D/PDGFR-β/nuclear factor κB (NF-κB) p65 in placenta tissues was evaluated by western blotting. Compared to the normal pregnant (NP) group, the levels of PDGF-D were augmented regardless of species. Knockdown of PDGF-D can result in the alleviation of HELLP syndrome development and progression in the HELLP rat model. Importantly, as a result of PDGF-D knockdown, the serum levels of inflammatory cytokines and oxidative stress activities were modulated, and the phosphorylation of PDGFR-β and NF-κB p65 in placenta tissue was inhibited. Taking together, our findings indicate that targeting PDGF-D could be used as a novel strategy to treat patients with HELLP syndrome.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Inhibiting PDGF-D alleviates the symptoms of HELLP by suppressing NF-κB activation

Wang¹,

et al. 2021

Journal of Molecular Endocrinology

View full text Add to dashboard Cite

show abstract

“…There is paucity in the literature regarding the mechanisms responsible for the cerebral complications that occur in HELLP syndrome. While we have previously reported that infusion of sFlt-1 and sEng into normal pregnant rats induces HELLP syndrome and increases systemic inflammation, endothelial activation, oxidative stress, BBB permeability, and neuroinflammation, we did not investigate the possible mechanisms of these neural changes [15,16,23]. In the current study, we have new data indicating that (1) myogenic tone is decreased in the middle cerebral arteries of HELLP rats, and (2) HELLP rats have cerebral edema.…”

Section: Discussionmentioning

confidence: 99%

“…On day twelve of gestation (GD12), 4.7 µg/kg/day of recombinant mouse sFlt-1 Fc Chimera (soluble fms-like tyrosine kinase-1) and 7 µg/kg/day of recombinant rat sEng Fc Chimera (soluble endoglin; R&D systems, Minneapolis, MN, USA) were infused into the abdominal cavity of normal pregnant rats via mini-osmotic pumps (Model 2002; Alzet Scientific, Cupertino, CA, USA) to create HELLP syndrome for a period of 8 days [12,15,16]. Rats not infused with either sFlt-1 or sEng served as normal pregnant (NP) controls.…”

Section: Methodsmentioning

confidence: 99%

Peripheral Anti-Angiogenic Imbalance during Pregnancy Impairs Myogenic Tone and Increases Cerebral Edema in a Rodent Model of HELLP Syndrome

et al. 2018

Self Cite

View full text Add to dashboard Cite

Using an animal model of hemolysis elevated liver enzymes low platelets (HELLP) that has systemic inflammation and neuroinflammation we wanted to determine if blood brain barrier (BBB) permeability, cerebral edema, vascular tone, and occludin expression were altered in pregnant rats. Anti-angiogenic proteins sFlt-1 and sEng (4.7 and 7 µg/kg/day, respectively) were chronically infused into normal pregnant (NP) rats beginning on gestational day 12 via a mini-osmotic pump. On gestational day 19, blood pressure was measured via a carotid catheter and brains were collected. BBB permeability was assessed in select brain regions from rats infused with 0.5 mg/mL Texas Red Dextran and phenylephrine. Occludin, sFlt-1, and sEng were analyzed via western blot or ELISA. Infusion of sFlt-1 and sEng into NP rats increased hemolysis and liver enzymes, and decreased platelets and led to hypertension. HELLP rats had significant impairment in the myogenic response and increased BBB permeability in the posterior cortex and brainstem. Brain water content in the posterior cortex was increased and sEng protein expression in the brainstem was significantly increased in HELLP rats. The results from this study suggest that a peripheral anti-angiogenic imbalance during pregnancy is associated with decreased myogenic tone, vasogenic edema, and an increase in BBB permeability, but not anti-angiogenic imbalance in the brain.

show abstract

“…63 This is mainly related to the abnormal production of reactive oxygen species (ROS), superoxides, and eNOS, which upset the balance between the oxidative and antioxidant systems. 64 Oxidative stress is crucial in CVD progression, 65 involving AS, 66 HF, 67 hypertension, 68 myocardial infarction, 69 and other diseases. Hawthorn is rich in anthocyanins, flavonoids, and phenolics, among which anthocyanins are considered active antioxidants.…”

Section: Vascular Protective Effects Of He In Cvdmentioning

confidence: 99%

Advances in the study of the vascular protective effects and molecular mechanisms of hawthorn (Crataegus anamesaSarg.) extracts in cardiovascular diseases

Zhang

Pan

et al. 2023

Food Funct.

View full text Add to dashboard Cite

show abstract

Attenuation of oxidative stress and hypertension in an animal model of HELLP syndrome

Cited by 7 publications

References 31 publications

Inhibiting PDGF-D alleviates the symptoms of HELLP by suppressing NF-κB activation

Inhibiting PDGF-D alleviates the symptoms of HELLP by suppressing NF-κB activation

Peripheral Anti-Angiogenic Imbalance during Pregnancy Impairs Myogenic Tone and Increases Cerebral Edema in a Rodent Model of HELLP Syndrome

Advances in the study of the vascular protective effects and molecular mechanisms of hawthorn (Crataegus anamesaSarg.) extracts in cardiovascular diseases

Contact Info

Product

Resources

About