Attenuation of Renal Ischemia-Reperfusion Injury in Rats by Allopurinol and Prostaglandin E&lt;sub&gt;1&lt;/sub&gt;

Gupta, Prachi; Matsushita, Mutsuyoshi; Oda, Kenichi; Nishikimi, Naomichi; Sakurai, Toshimitsu; Nimura, Yuji

doi:10.1159/000008564

Cited by 14 publications

(11 citation statements)

References 23 publications

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“…Experimental models of ischemia and reperfusion are long described and widely found in the literature 16,[19][20][21][22][23] .…”

Section: Discussionmentioning

confidence: 99%

“…[13][14][15] Some authors have also studied shorter clamping times with similar reperfusion periods with renal pedicle or single limb artery clamping, suggesting questionable benefit of its use. [17][18][19][20][21] We believe that these conflicting results are related to short clamping periods, some of what could even controversially work as preconditioning protective factor. Another major difference is the potential to create significant IRI, which is long known to be When comparing electrolytes, no statistically significant difference between groups on the levels of Sodium (p>0.05) and Calcium (p>0.05) were found.…”

Section: Discussionmentioning

confidence: 99%

“…Recent studies have suggested a potential protective role due to blocking of leukocyte chemoattractant factors, as well as weakening leukocyte adhesion to microvascular endothelium, with lesser tissue expression of some inflammatory markers involved in this process, in particular the Intercellular Adhesion Molecule 1 (ICAM-1), possibly leading to a cytoprotective activity also by lowering peripheral vascular resistance [20][21][22][23][24] .…”

Section: Introductionmentioning

confidence: 99%

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Alprostadil attenuates inflammatory aspects and leucocytes adhesion on renal ischemia and reperfusion injury in rats

et al. 2014

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. Tutor. Responsible for conception and critical revision of the study. ABSTRACT PURPOSE:To evaluate the effects of alprostadil in an experimental model of ischemia and reperfusion injury (IRI) in rat renal tissue. METHODS:Adult male Wistar rats were randomized into three groups Vehicle-treated group(Veh), Alprostadil-treated(Al), and sham(Sh) group. Veh and Al groups had suprarenal aorta occluded for 30 minutes and reperfused for 60 minutes. Saline or 20 µg/ kg of Alprostadil was intravenously infused immediately before declamping. Sh group animals underwent similar procedure without aortic occlusion. Left nephrectomy and blood sampling were performed after 60 minutes of reperfusion. Renal ICAM-1 expression and histological analysis were performed to estimate inflammatory response and tissue disarrangement. Serum biochemical markers for IRI were also measured. Kruskal-Wallis test was used to assess differences between the groups. RESULTS:There was lower expression of ICAM-1 in groups Veh and Sh. On histologically evaluation, inflammation and necrosis in the Veh group was significantly higher (grades III/IV) than Al group (Veh>Al=Sh; p = 0.025), as well as CPK levels (Veh>Al=Sh; p = 0.03). CONCLUSION:Alprostadil attenuates the immunohistochemical and histological repercussions in the renal tissue of rats submitted to a post-ischemic reperfusion with supra-renal aortic clamping.

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“…Experimental models of ischemia and reperfusion are long described and widely found in the literature 16,[19][20][21][22][23] .…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Alprostadil attenuates inflammatory aspects and leucocytes adhesion on renal ischemia and reperfusion injury in rats

et al. 2014

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“…Prostaglandins have been shown to be involved in the pathogenesis of post ischemic renal injury and a close interaction between eicosanoids and NO and their generation has been recently suggested [15]. Studies exploring the renal protective effect of PGE 1 in ischemia-reperfusion injury are few [16]. Although both NO and PGE 1 have been used as single agents to decrease renal reperfusion injury, there is no published work describing the simultaneous use of these agents in post ischemic injury.…”

Section: Introductionmentioning

confidence: 99%

Role of Combined L-Arginine and Prostaglandin E<sub>1</sub> in Renal Ischemia-Reperfusion Injury

et al. 2007

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Background:L-Arginine (L-arg) and Prostaglandin E₁ (PGE₁) have been used effectively as single agents to ameliorate renal ischemia-reperfusion injury. We hypothesized that combined treatment with L-arg and PGE₁would be more effective. Materials and Methods: The left renal artery of male Sprague-Dawley rats was clamped for 45 min and the right kidney was removed. Fifty six rats were randomly allocated into 5 groups each consisted of 12 rats except sham group (n = 8). (1) sham, underwent right nephrectomy only; (2) control, untreated ischemic rats; (3) L-arg group, L-arg-treated ischemic rats; (4) PGE₁ group, PGE₁-treated ischemic rats; (5) L-arg+PGE₁ group, ischemic rats treated with both L-arg and PGE₁. Renal function and histology were assessed on days 2 and 7 postoperatively. Results: All rats, except control ones, showed a significant improvement of renal function towards normal on postoperative day 7. Serum creatinine and creatinine clearance were significantly better in L-arg+PGE₁ group compared to all other groups on day 7. With the exception of sham-operated and L-arg+PGE₁-treated animals, all other groups showed significant increases in fractional excretion of sodium (FE_Na) in response to renal ischemia-reperfusion. The severest tubular damage was determined in the kidneys of control rats. Rats treated with L-arg+PGE₁ had the least severe tubular damage. Conclusion: The administration of either L-arg or PGE₁ attenuates both functional and structural consequences of renal warm ischemia. A near total protection might be achieved when both agents are administered concomitantly.

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“…Various substances that prevent the formation of free radicals have been studied, including xanthine oxidase inhibitors. Alopurinol, the best-known xanthine oxidase inhibitor, has been proved to be an effective agent 13,14 . This purine analog competes with purine for the active site on the enzyme.…”

Section: Introductionmentioning

confidence: 99%

The influence of methylene blue on the healing of intestinal anastomoses subjected to ischemia and reperfusion in rats

et al. 2010

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PURPOSE: To investigate the influence of methylene blue, on the healing of intestinal anastomoses subjected to ischemia and reperfusion in rats. METHODS: Forty-five rats divided into the following three groups were used: control (G1); ischemia without methylene blue (G2); and ischemia with methylene blue (G3). A laparotomy was performed and the cranial mesenteric artery isolated. Whereas the cranial artery was temporarily occluded for 45 minutes in groups G2 and G3, prior to enterotomy and intestinal anastomosis, in group G1 the enterotomy and intestinal anastomosis were performed without prior lesion. Afterwards, 2mL of 0.5% methylene blue were instilled in the peritoneal cavities of the animals in group G3, and 2mL of isotonic saline solution in the peritoneal cavities of the animals in group G2. After the reperfusion, an enterectomy and intestinal anastomosis were performed. After the animals had been sacrificed on the seventh day after the operation, the abdominal cavity was examined by resection of a segment of the intestine containing the anastomosis in order to measure its strength and for histopathological examination. RESULTS: Free fluid or abscesses in the peritoneal cavity were rare. When inflammation was analyzed, the group subjected to ischemia without methylene blue had a higher score for mononuclear cells (p=0.021) and granulation tissue (p=0.044). No significant difference was observed in the density of type I or type III collagens. CONCLUSION: The methylene blue did not show beneficial effect on the healing of intestinal anastomoses subjected to ischemia and reperfusion in rats.

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Attenuation of Renal Ischemia-Reperfusion Injury in Rats by Allopurinol and Prostaglandin E<sub>1</sub>

Cited by 14 publications

References 23 publications

Alprostadil attenuates inflammatory aspects and leucocytes adhesion on renal ischemia and reperfusion injury in rats

Alprostadil attenuates inflammatory aspects and leucocytes adhesion on renal ischemia and reperfusion injury in rats

Role of Combined L-Arginine and Prostaglandin E<sub>1</sub> in Renal Ischemia-Reperfusion Injury

The influence of methylene blue on the healing of intestinal anastomoses subjected to ischemia and reperfusion in rats

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